A0K1I7 (TRMB_ARTS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	Arth_3782

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000288119
Regions
	Region	298 – 301	4	Substrate binding Potential
Sites
	Binding site	103	1	S-adenosyl-L-methionine By similarity
	Binding site	128	1	S-adenosyl-L-methionine By similarity
	Binding site	155	1	S-adenosyl-L-methionine By similarity
	Binding site	178	1	S-adenosyl-L-methionine By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	214	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	A0K1I7 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 66612CE2339DD1A6	FASTA	319	34,907
	10 20 30 40 50 60 MSESPETPEP SPAQSPEAAP EQPQAARPVT PGSQASFGTY GGRPVSFVRR GTRLQGRRQQ 70 80 90 100 110 120 AWEEHSDRWA VDVPRHIANT SVHPDYVFDA EAEFGRSAPL IVEIGSGLGD AVCHAAEENP 130 140 150 160 170 180 DWDFLAVEVY TPGLANTVIK INSRKLSNVR VVEANAPEVL ATMLPAGSVS EVWVFFPDPW 190 200 210 220 230 240 HKSRHHKRRL IQPEFAALVA AALKPGGLFR VATDWSNYAV HVRDVMAGSA DFVNLHDGER 250 260 270 280 290 300 RGPESPLTQV WQSGVESLVG GAPVKEGRAP VSTEHTGPNE GVDETGGWAP RFEGRIRTSF 310 ENKAHEAGRL IFDLCYRRL

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FB24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000454 Genomic DNA. Translation: ABK05157.1.
RefSeq	YP_833257.1. NC_008541.1.
3D structure databases
ProteinModelPortal	A0K1I7.
ModBase	Search...
Protein-protein interaction databases
STRING	A0K1I7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4447832.
GenomeReviews	Gene locus Arth_3782 in contig CP000454_GR.
KEGG	art:Arth_3782.
PATRIC	21003065. VBIArtSp72239_4210.
TIGR	Arth_3782.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HBG322782.
OMA	VEANAPE.
PhylomeDB	A0K1I7.
ProtClustDB	CLSK960282.
Enzyme and pathway databases
BioCyc	ASP1667:ARTH_3782-MONOMER.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
KO	K03439.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_ARTS2

Accession

Primary (citable) accession number: A0K1I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program