Reviewed, UniProtKB/Swiss-Prot A0JZC7 (TYSY_ARTS2)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Arth_3018

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Thymidylate synthase HAMAP MF_00008		PRO_0000321466
Sites
	Active site	149	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JZC7-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 64DE7A588619AC66	FASTA	267	30,447
	10 20 30 40 50 60 MSIPTPYEDL LRDVLANGTH KSDRTGTGTL SVFGRQMRFD LSQSFPLITT KRVHFKSVAV 70 80 90 100 110 120 ELLWFLRGET NVKWMQDQGV TIWNEWADAD GELGPVYGVQ WRSWPTPDGG HIDQIAELVE 130 140 150 160 170 180 NLKSNPDSRR HIVSAWNVAE LQDMALPPCH AFFQFYVADG KLSCQLYQRS ADTFLGVPFN 190 200 210 220 230 240 IASYALLTCM LAQQVGLEPG EFVWTGGDVH IYDNHMDQVL KQLKREPYEY PQLKILRKPD 250 260 SIFDYTLDDF EVVGYQHHPT IKAPIAV

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000454 Genomic DNA. Translation: ABK04397.1.
RefSeq	YP_832497.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4444385.
GenomeReviews	Gene locus Arth_3018 in contig CP000454_GR.
KEGG	art:Arth_3018.
TIGR	Arth_3018.
Phylogenomic databases
OMA	A0JZC7. LGPVYGH.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_ARTS2

Accession

Primary (citable) accession number: A0JZC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)