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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Arthrobacter sp. (strain FB24)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115IronUniRule annotation1
Metal bindingi119IronUniRule annotation1
Binding sitei173SubstrateUniRule annotation1
Binding sitei186Substrate; via amide nitrogenUniRule annotation1
Binding sitei190SubstrateUniRule annotation1
Binding sitei291SubstrateUniRule annotation1
Metal bindingi319IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:Arth_2892
OrganismiArthrobacter sp. (strain FB24)
Taxonomic identifieri290399 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeArthrobacter
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003032581 – 356tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST356

Proteomic databases

PRIDEiA0JZ01

Interactioni

Protein-protein interaction databases

STRINGi290399.Arth_2892

Structurei

3D structure databases

ProteinModelPortaliA0JZ01
SMRiA0JZ01
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 143Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM Bacteria
COG0533 LUCA
HOGENOMiHOG000109568
KOiK01409
OMAiHLEGHIY
OrthoDBiPOG091H010B

Family and domain databases

HAMAPiMF_01445 TsaD, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR017861 KAE1/TsaD
IPR022450 TsaD
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF6 PTHR11735:SF6, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR00329 gcp_kae1, 1 hit
TIGR03723 T6A_TsaD_YgjD, 1 hit

Sequencei

Sequence statusi: Complete.

A0JZ01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRTQPLVLG IESSCDETGV GIVRGTALLS NTVSSSMEEH VRFGGVIPEI
60 70 80 90 100
ASRAHLDAFV PTLQEALADA GVQLDDVDAI AVTSGPGLAG ALMVGVCAAK
110 120 130 140 150
ALAVATGKPL YAINHLVAHV GVGLLQEENT LPEHLGALLV SGGHTEILRI
160 170 180 190 200
RSITDDVELL GSTIDDAAGE AYDKVARLLG LGYPGGPAID KLARTGNAKA
210 220 230 240 250
IRFPRGLTQP KYMGTADEPG PHRYDWSFSG LKTAVARCVE QFEARGDEVP
260 270 280 290 300
VADIAAAFQE AVVDVITSKA VLACTENGIT ELLLGGGVAA NSRLRQLTEQ
310 320 330 340 350
RCRAAGIRLT VPPLELCTDN GAMVAALGAQ LVMAGIEPSG ISFAPDSSMP

VTTVSA
Length:356
Mass (Da):36,793
Last modified:December 12, 2006 - v1
Checksum:i538FBCF34794DCAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA Translation: ABK04271.1
RefSeqiWP_011692730.1, NC_008541.1

Genome annotation databases

EnsemblBacteriaiABK04271; ABK04271; Arth_2892
KEGGiart:Arth_2892

Entry informationi

Entry nameiTSAD_ARTS2
AccessioniPrimary (citable) accession number: A0JZ01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 12, 2006
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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