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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Arthrobacter sp. (strain FB24)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151IronUniRule annotation
Metal bindingi119 – 1191IronUniRule annotation
Binding sitei173 – 1731SubstrateUniRule annotation
Binding sitei186 – 1861Substrate; via amide nitrogenUniRule annotation
Binding sitei190 – 1901SubstrateUniRule annotation
Binding sitei291 – 2911SubstrateUniRule annotation
Metal bindingi319 – 3191IronUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciASP290399:GHIF-2959-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:Arth_2892
OrganismiArthrobacter sp. (strain FB24)
Taxonomic identifieri290399 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeArthrobacter
Proteomesi
  • UP000000754 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000303258Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290399.Arth_2892.

Structurei

3D structure databases

ProteinModelPortaliA0JZ01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 1435Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
KOiK01409.
OMAiRDHVKRM.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.

Sequencei

Sequence statusi: Complete.

A0JZ01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRTQPLVLG IESSCDETGV GIVRGTALLS NTVSSSMEEH VRFGGVIPEI
60 70 80 90 100
ASRAHLDAFV PTLQEALADA GVQLDDVDAI AVTSGPGLAG ALMVGVCAAK
110 120 130 140 150
ALAVATGKPL YAINHLVAHV GVGLLQEENT LPEHLGALLV SGGHTEILRI
160 170 180 190 200
RSITDDVELL GSTIDDAAGE AYDKVARLLG LGYPGGPAID KLARTGNAKA
210 220 230 240 250
IRFPRGLTQP KYMGTADEPG PHRYDWSFSG LKTAVARCVE QFEARGDEVP
260 270 280 290 300
VADIAAAFQE AVVDVITSKA VLACTENGIT ELLLGGGVAA NSRLRQLTEQ
310 320 330 340 350
RCRAAGIRLT VPPLELCTDN GAMVAALGAQ LVMAGIEPSG ISFAPDSSMP

VTTVSA
Length:356
Mass (Da):36,793
Last modified:December 12, 2006 - v1
Checksum:i538FBCF34794DCAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK04271.1.
RefSeqiWP_011692730.1. NC_008541.1.

Genome annotation databases

EnsemblBacteriaiABK04271; ABK04271; Arth_2892.
KEGGiart:Arth_2892.
PATRICi21001195. VBIArtSp72239_3280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK04271.1.
RefSeqiWP_011692730.1. NC_008541.1.

3D structure databases

ProteinModelPortaliA0JZ01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290399.Arth_2892.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK04271; ABK04271; Arth_2892.
KEGGiart:Arth_2892.
PATRICi21001195. VBIArtSp72239_3280.

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
KOiK01409.
OMAiRDHVKRM.
OrthoDBiEOG6K402S.

Enzyme and pathway databases

BioCyciASP290399:GHIF-2959-MONOMER.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FB24.

Entry informationi

Entry nameiTSAD_ARTS2
AccessioniPrimary (citable) accession number: A0JZ01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 12, 2006
Last modified: December 9, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.