A0JYX8 (A0JYX8_ARTS2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344
EC=6.3.5.2 HAMAP MF_00344

Alternative name(s):
GMP synthetase HAMAP MF_00344
Glutamine amidotransferase HAMAP MF_00344

Gene names

Name:	guaA HAMAP MF_00344
Ordered Locus Names:	Arth_2869

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP] EMBL ABK04248.1

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 GMP-binding domain. HAMAP MF_00344 Contains 1 glutamine amidotransferase type-1 domain. HAMAP MF_00344

Ontologies

Keywords
Biological process	GMP biosynthesis HAMAP MF_00344 Purine biosynthesis
Domain	Glutamine amidotransferase HAMAP MF_00344
Ligand	ATP-binding HAMAP MF_00344 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00344 EMBL ABK04248.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	13 – 204	192	Glutamine amidotransferase type-1 By similarity HAMAP MF_00344
	Domain	236 – 404	169	GMP-binding By similarity HAMAP MF_00344
	Nucleotide binding	232 – 238	7	ATP By similarity HAMAP MF_00344
Sites
	Active site	90	1	Nucleophile By similarity HAMAP MF_00344
	Active site	178	1	By similarity HAMAP MF_00344
	Active site	180	1	By similarity HAMAP MF_00344

Sequences

	Sequence		Length	Mass (Da)
	A0JYX8 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 2BAC022C4DE6F4C2	FASTA	529	56,788
	10 20 30 40 50 60 MTTPTASQTS QKPVLVVDYG AQYAQLIARR VREANVYSEV VPHTYSTEQL LAKNPAAIIL 70 80 90 100 110 120 SGGPSSVYAE GAPSVGADLF EAGIPVFGIC YGFQAMANAL GGTVAQTGLR EYGATEATTV 130 140 150 160 170 180 GEARSILDGL PHHQNTWMSH GDSVQEAPEG FEVLATTAGA PVAAFANEEK CLYGVQWHPE 190 200 210 220 230 240 VKHSAYGQQV LENFLFKGAK LEPNWTTGNI LEEQVDRIRK QIGDARVICG LSGGVDSAVA 250 260 270 280 290 300 AALVQRAVGD QLTCVFVDHG LLREGEAEQV ERDFVAATGV KLYVANEQER FQAALAGVSD 310 320 330 340 350 360 PETKRKIIGR EFIRAFEEAE LAIIAEAAAH GEKIKFLVQG TLYPDVVESG GGEGAANIKS 370 380 390 400 410 420 HHNVGGLPED LQFELVEPLR ALFKDEVRAV GAQLGLPQEI VGRQPFPGPG LGIRIVGEVT 430 440 450 460 470 480 KERLDLLRKA DAIARAELTA AGLDNEVWQM PVVLLADVRS VGVQGDGRTY GHPIVLRPVS 490 500 510 520 SEDAMTADWS RLPYELLAKI SNRITNEVEG VNRVVLDVTS KPPGTIEWE

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FB24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000454 Genomic DNA. Translation: ABK04248.1.
RefSeq	YP_832348.1. NC_008541.1.
3D structure databases
ProteinModelPortal	A0JYX8.
ModBase	Search...
Protein-protein interaction databases
STRING	A0JYX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4444702.
GenomeReviews	Gene locus Arth_2869 in contig CP000454_GR.
KEGG	art:Arth_2869.
PATRIC	21001145. VBIArtSp72239_3255.
TIGR	Arth_2869.
Phylogenomic databases
eggNOG	COG0519.
HOGENOM	HBG391775.
OMA	DGRTYEY.
ProtClustDB	PRK00074.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A0JYX8_ARTS2

Accession

Primary (citable) accession number: A0JYX8

Entry history

Integrated into UniProtKB/TrEMBL:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)