A0JXZ9 (ILVD_ARTS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Arth_2540

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000321591
Sites
	Metal binding	135	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	212	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A0JXZ9 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 0711709D24A74E3C	FASTA	573	60,031
	10 20 30 40 50 60 MSEDTQTATE NKPDIKPRSR VVTDGIHAAP ARGMFRAVGM GDDDFAKPQI GVASSWNEIT 70 80 90 100 110 120 PCNLSLNRLA QGAKEGVHAG GGFPMQFGTI SVSDGISMGH EGMHFSLVSR EVIADSVETV 130 140 150 160 170 180 MQAERIDGSV LLAGCDKSLP GMLMAAARLD LASVFLYAGS IMPGWVKLED GSEKEVTLID 190 200 210 220 230 240 AFEAVGACAA GKMSRGDLDR IERAICPGEG ACGGMYTANT MACIGEALGM SLPGSAAPPS 250 260 270 280 290 300 ADRRRDEFAR KSGEAVVNLL RLGITARDIM TKKAFENAIA VTMAFGGSTN AVLHLLAIAR 310 320 330 340 350 360 EAEVELTLDD FNRIGDKIPH LGDLKPFGRY VMTDVDKIGG VPVIMKALLD AGLLHGDCLT 370 380 390 400 410 420 VTGKTLAENL ASINPPDLDG KILRALDNPI HKTGGITILH GSMAPEGAVV KSAGFDADVF 430 440 450 460 470 480 EGTARVFERE QGALDALDNG KINKGDVVVI RYEGPKGGPG MREMLAITGA IKGAGLGKDV 490 500 510 520 530 540 LLLTDGRFSG GTTGLCIGHV APEAVDGGPI AFVKDGDRIR VDIAARSFDL LVDEAELESR 550 560 570 KVGWEPLPAK FTKGVLAKYA KLVHSASTGA YCG

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FB24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000454 Genomic DNA. Translation: ABK03919.1.
RefSeq	YP_832019.1. NC_008541.1.
3D structure databases
ProteinModelPortal	A0JXZ9.
ModBase	Search...
Protein-protein interaction databases
STRING	A0JXZ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4444948.
GenomeReviews	Gene locus Arth_2540 in contig CP000454_GR.
KEGG	art:Arth_2540.
PATRIC	21000455. VBIArtSp72239_2916.
TIGR	Arth_2540.
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	NMPGAMI.
PhylomeDB	A0JXZ9.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	ASP1667:ARTH_2540-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ARTS2

Accession

Primary (citable) accession number: A0JXZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program