Reviewed, UniProtKB/Swiss-Prot A0JXX3 (DDL_ARTS2)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	Arth_2514

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00047
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP MF_00047
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047
Subcellular location	Cytoplasm By similarity HAMAP MF_00047.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	D-alanine--D-alanine ligase HAMAP MF_00047		PRO_0000341054
Regions
	Domain	161 – 372	212	ATP-grasp
	Nucleotide binding	193 – 248	56	ATP By similarity
Sites
	Metal binding	326	1	Magnesium or manganese 1 By similarity
	Metal binding	339	1	Magnesium or manganese 1 By similarity
	Metal binding	339	1	Magnesium or manganese 2 By similarity
	Metal binding	341	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JXX3-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 8D253FD6176B54FB	FASTA	382	40,775
	10 20 30 40 50 60 MSEEQLNKHT APGHAKPRVA VLFGGRSSEH AVSCVTAAGV LGAIDYDKYD VIPIGIAKTG 70 80 90 100 110 120 QWVLVSGDTR QWSLSASSLP EVSPSARTVT LAEIGGEHQL IVASPNEVPQ ELGAVDVVFP 130 140 150 160 170 180 LLHGPFGEDG TIQGLLELSD TRYVGAGVLA SAVGMDKHYM KVVFEAAGLQ VGPYIAVTDR 190 200 210 220 230 240 QWLTDPEAIR KRVDRLGYPV FVKPARAGSS MGISKVDSLE GLDAAIAAAR EHDLKLVIEA 250 260 270 280 290 300 GIVGREIECA VLEGRGTEPP RTSMPGEIAV APGEHEFYDF NAKYVEDDAA SLSCPADLPD 310 320 330 340 350 360 EAIARVRELA AAAFDAVGAE GLSRVDFFYT PDGELIINEI NTMPGFTPKS MYPQMWAASG 370 380 LGYAELIDEL IYLALNRKTG LR

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000454 Genomic DNA. Translation: ABK03893.1.
RefSeq	YP_831993.1.
3D structure databases
SMR	A0JXX3. Positions 16-378.
ModBase	Search...
Protein-protein interaction databases
STRING	A0JXX3.
Genome annotation databases
GeneID	4444920.
GenomeReviews	Gene locus Arth_2514 in contig CP000454_GR.
KEGG	art:Arth_2514.
TIGR	Arth_2514.
Phylogenomic databases
eggNOG	COG1181.
HOGENOM	HBG427092.
OMA	FTDISMY.
PhylomeDB	A0JXX3.
Family and domain databases
HAMAP	MF_00047. Dala_Dala_lig. [Tree]
InterPro	IPR011761. ATP-grasp. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp-like. [Graphical view]
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_ARTS2

Accession

Primary (citable) accession number: A0JXX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	December 12, 2006
Last modified:	January 19, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)