Reviewed, UniProtKB/Swiss-Prot A0JXT9 (TRMD_ARTS2)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Arth_2480

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000006448
Regions
	Region	140 – 145	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	116	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JXT9-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: D6F5E5FFD81B8E5B	FASTA	274	30,085
	10 20 30 40 50 60 MRIDVVSIFP EYLAPLELSL IGKARQDGIL DLNVHDLREF TTDKHRSVDD TPYGGGAGMV 70 80 90 100 110 120 MKAEPWAQAL STVAAARAGD AARKPVLIVP SPAGERFTQA LAHELAGEEQ LVFACGRYEG 130 140 150 160 170 180 IDERVIEWAE EHFTVRPMSL GDYVLNGGEV AVLAMTEAIV RLLPGVVGNP ESLVEESHSD 190 200 210 220 230 240 GLLEYPVYTK PSSWRDREVP PVLLSGNHGK IAQWRRHQQY RRTAQRRPDL LAEFDAGNLP 250 260 270 RADRTALGDL GYDVVDGHLR HRPEEPGNGA PADG

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000454 Genomic DNA. Translation: ABK03859.1.
RefSeq	YP_831959.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0JXT9.
Genome annotation databases
GeneID	4445069.
GenomeReviews	Gene locus Arth_2480 in contig CP000454_GR.
KEGG	art:Arth_2480.
TIGR	Arth_2480.
Phylogenomic databases
OMA	HRSVDDT.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ARTS2

Accession

Primary (citable) accession number: A0JXT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)