A0JXH5 (A0JXH5_ARTS2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172

Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172

Gene names

Name:	metE HAMAP MF_00172
Ordered Locus Names:	Arth_2366

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP] EMBL ABK03745.1

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	786 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis HAMAP MF_00172
Domain	Repeat HAMAP MF_00172
Ligand	Metal-binding HAMAP MF_00172 Zinc HAMAP MF_00172
Molecular function	Methyltransferase HAMAP MF_00172 EMBL ABK03745.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	668	1	Zinc By similarity HAMAP MF_00172
	Metal binding	670	1	Zinc By similarity HAMAP MF_00172
	Metal binding	753	1	Zinc By similarity HAMAP MF_00172

Sequences

	Sequence		Length	Mass (Da)
	A0JXH5 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: D09706F11A281081	FASTA	786	84,851
	10 20 30 40 50 60 MTEQNTPAVT TPFPSASILG YPRIGRRREL KKAVEAYWAG KIDAAALDAA AKEIQLGTAT 70 80 90 100 110 120 RLQGLGLTEA AAVPGTFSFY DQVLDAAAHL GAVPARFGNL LNAEGQLDID GYFTLARGNK 130 140 150 160 170 180 EEQPLEMTKW FDTNYHYLVP EIGPETNFAL TSNRIVEEFE YALANGVETR PYIVGPVTFL 190 200 210 220 230 240 LLSKASDDAP AGFSPLSRLE DILPVYTALL EKLAAAGASW VQLDEPALVV DQDTSAEEIQ 250 260 270 280 290 300 AAVARSYEVL SAAGRRPQLF VSTPYGALNG QLGTLAATNI DALHVDVFKG AVPSAAALAA 310 320 330 340 350 360 LGNKTLVAGV VDGHNIWRND LAASAEKIAE LKKSVTKLAI STSTSTQHVP HDVEEETQLS 370 380 390 400 410 420 EQLRSWLAFA DQKAVEVATL AGLLTDAAGS ETGSVQSAID EATRIIASRA AAEGVQRADV 430 440 450 460 470 480 RARTAALTAA DFNRSEYPVR EAAQEEALHL PPLPTTTIGS FPQTSEIRSA RARNNKGDLT 490 500 510 520 530 540 NEQYEQLMKD EIKRVVELQE ELGYDVLVHG EPERNDMVQY FAENLEGFDV TVHGWVQSYG 550 560 570 580 590 600 SRCTRPSILW GDVTRSAPIT VKWAEYAQSL TSKPMKGMLT GPVTILAWSF VRDDQPLGET 610 620 630 640 650 660 ANQVGLALRD EIADLEAAGI KVIQVDEPAL RELLPLRKAD QAAYLDWSVN SFRLATAGAA 670 680 690 700 710 720 DSTQIHTHLC YSEFGAIIDA IDGLDADVTS IEAARSRMEV VHDLESHGFG RGVGPGVYDI 730 740 750 760 770 780 HSPRVPGEQE VTELLSTAVK HVPSRQLWVN PDCGLKTRGY AETEESLRNL VAATKTVRAS LLATAK

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FB24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000454 Genomic DNA. Translation: ABK03745.1.
RefSeq	YP_831845.1. NC_008541.1.
3D structure databases
ProteinModelPortal	A0JXH5.
ModBase	Search...
Protein-protein interaction databases
STRING	A0JXH5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4445131.
GenomeReviews	Gene locus Arth_2366 in contig CP000454_GR.
KEGG	art:Arth_2366.
PATRIC	21000077. VBIArtSp72239_2736.
TIGR	Arth_2366.
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	A0JXH5.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0JXH5_ARTS2

Accession

Primary (citable) accession number: A0JXH5

Entry history

Integrated into UniProtKB/TrEMBL:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)