A0JXC7 (SYT_ARTS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Arth_2317

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 669	669	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000020338
Regions
	Region	260 – 566	307	Catalytic HAMAP MF_00184
Sites
	Metal binding	365	1	Zinc; catalytic By similarity
	Metal binding	416	1	Zinc; catalytic By similarity
	Metal binding	543	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JXC7 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 21BE16FDEBC45439	FASTA	669	74,930
	10 20 30 40 50 60 MSDAQQITLI VDGEETKVTT GTTGAELFFE RRDVVVARVN GELKDLDQPL PEGADIEGVT 70 80 90 100 110 120 IDSPDGLNVL RHSTAHVMAQ AVQQLRPDAK LGIGPYITDG FYFDFDVAEP FTPEDLKALE 130 140 150 160 170 180 KMMLKIINQN QKFVRRVVSE DEAREAMKNE PYKLELLGKK NNAADAGEGV NVEVGAGDIT 190 200 210 220 230 240 IYDNVDRKSG DSVWCDLCRG PHLPNTKLIS NAFALTRSSA AYWLGNQNNQ QLQRIYGTAW 250 260 270 280 290 300 PTKDALKAYQ ERIAEAERRD HRKLGAELDL FSFPDELGSG LPVFHPKGGI IRKAMEDYSR 310 320 330 340 350 360 QRHVDAGYDF VYTPHITKGH LYEVSGHLDW YKEGMFPAMH IDAELNEDGT VRKPGQDYYL 370 380 390 400 410 420 KPMNCPMHNL IFRSRGRSYR ELPLRLFEFG SVYRYEKSGV VHGLTRVRGM TQDDAHIYCT 430 440 450 460 470 480 REQMKDELTT TLNFVLGLLK DYGLDDFYLE LSTKNEDKFV GDDATWDEAT RTLAEVAEAS 490 500 510 520 530 540 GLELIPDPGG AAFYGPKISV QAKDALGRTW QMSTIQLDFN LPERFELEFQ AADGTRQRPV 550 560 570 580 590 600 MIHRALFGSV ERFMGVLTEH YAGAFPAWLA PVQVVGIPVA EAFNDYMFDV VDQLKAVGIR 610 620 630 640 650 660 AEVDISSDRF PKKIRTASKD KIPFVLIAGG EDAEAGAVSF RFRDGSQDNG VPVAEAVQRI VEAVRNRTS

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FB24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000454 Genomic DNA. Translation: ABK03697.1.
RefSeq	YP_831797.1. NC_008541.1.
3D structure databases
ProteinModelPortal	A0JXC7.
ModBase	Search...
Protein-protein interaction databases
STRING	A0JXC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4445360.
GenomeReviews	Gene locus Arth_2317 in contig CP000454_GR.
KEGG	art:Arth_2317.
PATRIC	20999977. VBIArtSp72239_2686.
TIGR	Arth_2317.
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HBG352811.
OMA	GRKWQLG.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	ASP1667:ARTH_2317-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ARTS2

Accession

Primary (citable) accession number: A0JXC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program