A0JXA1 (SYD_ARTS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Arth_2291

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000006633

Sequences

	Sequence		Length	Mass (Da)
	A0JXA1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: C92073621960756E	FASTA	596	65,331
	10 20 30 40 50 60 MLRTHDLGSL RSEHIGQTVT LAGWVGRRRD HGGVAFVDLR DASGVSQVVV REEEVFHGLR 70 80 90 100 110 120 NEYVLQVIGT VSQRPEGNEN PALATGQIEV IAEKVTILNT SDPLPFQIDE HVEVGEEARL 130 140 150 160 170 180 KHRYLDLRRP GPARNMRLRS EANRVARELL HRDGYVEIET PTLTRSTPEG ARDFVVPARL 190 200 210 220 230 240 APGSWYALPQ SPQLFKQLLQ VGGFEKYYQI ARCYRDEDFR ADRQPEFTQL DIEASFVEQD 250 260 270 280 290 300 DIISLGESIV KALWQLIDVE IPTPIQRITY ADAMARYGSD KPDLRFGLEL TELTEFFKDT 310 320 330 340 350 360 NFGVFKAPYV GAVVMPGGAS QARRALDAWQ EWAKQRGAKG LAYVLFKEDG ELAGPVAKNL 370 380 390 400 410 420 TDTERAGLAD AVGAKPGDCI FFAAGEKTPS RALLGAARVE IGHRTGLINP ADWAFCWVVD 430 440 450 460 470 480 APMFEPAAAA VASGDVAVGA GQWTAVHHAF TSPKPEFMDS FDKDPESALS YAYDIVCNGN 490 500 510 520 530 540 EIGGGSIRIH ERDVQERVFE LMGLDKADAE TKFGFLLEGF KFGAPPHGGI AFGWDRVVAL 550 560 570 580 590 LAGVESIRDV IAFPKSGGGY DPLTQAPAPI TAQQRKEAGV DFKPEAKKAD PGATKA

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FB24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000454 Genomic DNA. Translation: ABK03671.1.
RefSeq	YP_831771.1. NC_008541.1.
3D structure databases
ProteinModelPortal	A0JXA1.
ModBase	Search...
Protein-protein interaction databases
STRING	A0JXA1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4445334.
GenomeReviews	Gene locus Arth_2291 in contig CP000454_GR.
KEGG	art:Arth_2291.
PATRIC	20999925. VBIArtSp72239_2660.
TIGR	Arth_2291.
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HBG396032.
OMA	AFPKTQQ.
PhylomeDB	A0JXA1.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	ASP1667:ARTH_2291-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ARTS2

Accession

Primary (citable) accession number: A0JXA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program