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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Arthrobacter sp. (strain FB24)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei609 – 6091ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciASP290399:GHIF-2293-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Arth_2247
OrganismiArthrobacter sp. (strain FB24)
Taxonomic identifieri290399 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
ProteomesiUP000000754: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 843843Leucine--tRNA ligasePRO_0000334729Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290399.Arth_2247.

Structurei

3D structure databases

ProteinModelPortaliA0JX57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7111"HIGH" regionAdd
BLAST
Motifi606 – 6105"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JX57-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVQPETETG TAAVATEGPE EGTYSFAAME AKWPQVWEDL KVFTPVDDGS
60 70 80 90 100
RERRYVLDMF PYPSGDLHMG HAEAFAMGDV VARYLRQKGY DVLHPIGWDS
110 120 130 140 150
FGLPAENAAI KRNAHPSEWT YANIDTQAAS FKRYAISADW SRRLHTSDPE
160 170 180 190 200
YYRWTQWLFK RFYERGLAYR KDSPVNWCPK DQTVLANEQV VNGACERCGT
210 220 230 240 250
AVTKKSLNQW YFKITDYADR LLDDMDELRG HWPERVLAMQ KNWIGRSEGA
260 270 280 290 300
HVNFVIEAAG DKPAKDVTVF TTRPDTLYGA TFFVVAADAP MAVELVTDEH
310 320 330 340 350
AAALDAYREQ VKALSEIERQ STEREKTGVF TGRYAVNPLN GEKLPVWAAD
360 370 380 390 400
YVLADYGTGA IMAVPAHDQR DLDFARTFDL PVRAVLDTGE DDPAVTGKAT
410 420 430 440 450
AGEGTLINSG VLDGLPKAEA IPTAIDMLEK QGTGEKFVNF RLRDWLLSRQ
460 470 480 490 500
RFWGTPIPII HCPACGEVPV PDDQLPVTLP ADLRGEDLSP KGTSPLAAAE
510 520 530 540 550
AWVNVECPNC HGPAKRDTDT MDTFVDSSWY FLRFVSPQYT EGPFDPKKIN
560 570 580 590 600
DWMPVGQYVG GVEHAILHLL YARFFTKVIH DLGMIDADEP FSALLNQGQV
610 620 630 640 650
LNGGKAMSKS LGNGVDLGEQ LDKYGVDAVR LTMIFASPPE DDVDWADVSP
660 670 680 690 700
SGSAKFLARA WRLAQDVSSA PGADAAQGDR ALRSVTHRTI ADAAALLDSN
710 720 730 740 750
KFNVVVAKLM ELVNATRKTI DAASGAGGAD PAVREAAEAV AVILSLFAPY
760 770 780 790 800
TAEDMWNVLG HPASVANAGW PSHDEALLVQ DTVTAVVQVQ GKVRDRLEVS
810 820 830 840
PEIGEDELRE LALASENVQR ALDGRGIRTV IVRAPKLVNI VPA
Length:843
Mass (Da):92,600
Last modified:December 12, 2006 - v1
Checksum:i9C5CE6DF8B5BFEB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK03627.1.
RefSeqiWP_011692091.1. NC_008541.1.
YP_831727.1. NC_008541.1.

Genome annotation databases

EnsemblBacteriaiABK03627; ABK03627; Arth_2247.
GeneIDi4445169.
KEGGiart:Arth_2247.
PATRICi20999837. VBIArtSp72239_2616.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK03627.1.
RefSeqiWP_011692091.1. NC_008541.1.
YP_831727.1. NC_008541.1.

3D structure databases

ProteinModelPortaliA0JX57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290399.Arth_2247.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK03627; ABK03627; Arth_2247.
GeneIDi4445169.
KEGGiart:Arth_2247.
PATRICi20999837. VBIArtSp72239_2616.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciASP290399:GHIF-2293-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FB24.

Entry informationi

Entry nameiSYL_ARTS2
AccessioniPrimary (citable) accession number: A0JX57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 12, 2006
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.