Reviewed, UniProtKB/Swiss-Prot A0JX31 (PYRD_ARTS2)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	Arth_2221

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_0000336453
Sites
	Active site	189	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JX31-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: ABD678D314C22CB4	FASTA	357	38,147
	10 20 30 40 50 60 MRVYPTFFRL AFSWMDAERA HRIGFRAIRL AHSSGAGRVL AKFTAPAPSL QTTAFGITFP 70 80 90 100 110 120 SPFGLAAGFD KEGHGIEALT ELGFGHVEVG TITGQAQPGN EKPRLFRLVE DKAVINRMGF 130 140 150 160 170 180 NNDGATAVAP RLKSARAALQ RRHPGVRPVI GVNIGKSKVV ELEDAASDYL VSARSLAPAA 190 200 210 220 230 240 DYLVVNVSSP NTPGLRLLQN VETLRPLLKA VGEEADKAAG RHVPLLVKIA PDLTDEDIDD 250 260 270 280 290 300 VAKLALDLGL DGIIATNTTI GREGLTSDPE KIRDCGPGGL SGAPLKARSL EVLRRLKEAT 310 320 330 340 350 GGSLTLVSVG GVENARDVQE RLDAGATLVQ GYTAFLYEGP FWAARINRQL AKHPARR

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000454 Genomic DNA. Translation: ABK03601.1.
RefSeq	YP_831701.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4445282.
GenomeReviews	Gene locus Arth_2221 in contig CP000454_GR.
KEGG	art:Arth_2221.
TIGR	Arth_2221.
Phylogenomic databases
OMA	A0JX31. AALNRMG.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ARTS2

Accession

Primary (citable) accession number: A0JX31

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)