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Protein

UvrABC system protein B

Gene

uvrB

Organism
Arthrobacter sp. (strain FB24)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 55ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease, Helicase, Hydrolase
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:Arth_2070
OrganismiArthrobacter sp. (strain FB24)
Taxonomic identifieri290399 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeArthrobacter
Proteomesi
  • UP000000754 Componenti: Chromosome

Subcellular locationi

A0JWN0:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000778651 – 693UvrABC system protein BAdd BLAST693

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi290399.Arth_2070.

Structurei

3D structure databases

ProteinModelPortaliA0JWN0.
SMRiA0JWN0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 188Helicase ATP-bindingUniRule annotationAdd BLAST154
Domaini438 – 604Helicase C-terminalUniRule annotationAdd BLAST167
Domaini648 – 683UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi101 – 124Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
KOiK03702.
OMAiRYMHSEI.
OrthoDBiPOG091H02LK.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
Gene3Di4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiView protein in InterPro
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR036876. UVR_dom_sf.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.

Sequencei

Sequence statusi: Complete.

A0JWN0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLAQEINRV VAPFEVISEF KPAGDQPAAI AELTERIKNG EKDVVLLGAT
60 70 80 90 100
GTGKSATTAW LIEQVQRPTL VMVQNKTLAA QLANEFRELL PNNAVEYFVS
110 120 130 140 150
YYDYYQPEAY VAQTDTFIEK DSSVNEEVER LRHSATNALL TRRDVIVVAT
160 170 180 190 200
VSCIYGLGTP EEYIAGMVTL RKGAEMNRDD LLRKFVSMQY ARNDMDFHRG
210 220 230 240 250
TFRVRGDTVE IIPMYEELAI RIEFFGDEIE NIHTLHPLTG EVIRDEEEMY
260 270 280 290 300
VFPASHYVAG PERMSRAIKR IEDELAERLQ VLESQNKLVE AQRLRMRTTY
310 320 330 340 350
DLEMMQQMGF CNGIENYSSH IDGRARGTAP HCLLDYFPDD FLLVIDESHV
360 370 380 390 400
TVPQIGAMYE GDMSRKRNLV DFGFRLPSAM DNRPLKWDEF QERVGQTVYL
410 420 430 440 450
SATPGKYELG KSDGFVQQII RPTGLIDPEV VVKPTKGQID DLLGEIKTRT
460 470 480 490 500
AKNERVLVTT LTKRMAEDLT DYLLGHGVKV EYLHSDVDTL RRVELLRELR
510 520 530 540 550
MGVFDVLVGI NLLREGLDLP EVSLVSILDA DKEGFLRSST SLIQTIGRAA
560 570 580 590 600
RNVSGQVHMY ADRITDSMAH AIDETNRRRA IQVAYNTEHG IDPQPLRKKI
610 620 630 640 650
ADITDQLAKE DADTRELLAA SGKTRGKGKG ASTVRADGLA AAPAEDLVGL
660 670 680 690
IEQLTEQMHA AAGELQFELA ARLRDEVGEL KKELRQMQAA GHA
Length:693
Mass (Da):77,898
Last modified:December 12, 2006 - v1
Checksum:iC0CE374BE886F540
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK03450.1.
RefSeqiWP_011691916.1. NC_008541.1.

Genome annotation databases

EnsemblBacteriaiABK03450; ABK03450; Arth_2070.
KEGGiart:Arth_2070.

Similar proteinsi

Entry informationi

Entry nameiUVRB_ARTS2
AccessioniPrimary (citable) accession number: A0JWN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 12, 2006
Last modified: October 25, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families