A0JV26 (ASSY_ARTS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate synthase
EC=6.3.4.5

Alternative name(s):
Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	Arth_1502

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity. HAMAP MF_00005
Subcellular location	Cytoplasm By similarity HAMAP MF_00005.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW argininosuccinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Argininosuccinate synthase HAMAP MF_00005		PRO_1000000380
Regions
	Nucleotide binding	8 – 16	9	ATP By similarity
Sites
	Binding site	87	1	Citrulline By similarity
	Binding site	117	1	ATP; via amide nitrogen By similarity
	Binding site	119	1	Aspartate By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	123	1	Citrulline By similarity
	Binding site	124	1	Aspartate By similarity
	Binding site	127	1	Citrulline By similarity
	Binding site	175	1	Citrulline By similarity
	Binding site	259	1	Citrulline By similarity
	Binding site	271	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JV26 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 865D6E340EBC95B0	FASTA	401	43,633
	10 20 30 40 50 60 MTERIVLAYS GGLDTSVAIG WIGEATGAEV IAVAVDVGQG GESLETIRQR ALGCGAVEAY 70 80 90 100 110 120 VADASDEFAN EYCMPTLKAN ALYQGHYPLV SAISRPVIVK HLVKAAREFG ATTVAHGCTG 130 140 150 160 170 180 KGNDQVRFEV GIQTLGPDLK CIAPVRDLAL TRDKAIAFAE EKGLPIETTK KNPYSIDQNV 190 200 210 220 230 240 WGRAVETGYL EDIWNAPTKD IYDYTATPEF PPAPDEVIIS FEAGVPVAID GVRVTPLQAI 250 260 270 280 290 300 KELNRRAGAQ GVGRIDVVED RLVGIKSREI YEAPGAMALI TAHKHLEDIT VEREQARFKA 310 320 330 340 350 360 TVGQRWSELV YDGQWFSPLK RSLDAFIEDT QKYVSGDIRM TLHGGQAVVN GRRSETSLYD 370 380 390 400 FDLATYDTGD TFDQSMARGF IELWGMSAKV ASGRDIRVAG K

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FB24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000454 Genomic DNA. Translation: ABK02896.1.
RefSeq	YP_830996.1. NC_008541.1.
3D structure databases
ProteinModelPortal	A0JV26.
ModBase	Search...
Protein-protein interaction databases
STRING	A0JV26.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4445998.
GenomeReviews	Gene locus Arth_1502 in contig CP000454_GR.
KEGG	art:Arth_1502.
PATRIC	20998301. VBIArtSp72239_1860.
TIGR	Arth_1502.
Phylogenomic databases
eggNOG	COG0137.
HOGENOM	HBG335267.
OMA	LATYDTG.
PhylomeDB	A0JV26.
ProtClustDB	PRK00509.
Enzyme and pathway databases
BioCyc	ASP1667:ARTH_1502-MONOMER.
Family and domain databases
HAMAP	MF_00005. Arg_succ_synth_type1. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR023434. Arginosuc_synth_type_1_subfam. IPR024074. AS_cat/multimer_dom_body. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.90.1260.10. G3DSA:3.90.1260.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01940.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. ArgG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_ARTS2

Accession

Primary (citable) accession number: A0JV26

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program