Reviewed, UniProtKB/Swiss-Prot A0JUZ2 (LEXA_ARTS2)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	Arth_1468

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	LexA repressor HAMAP MF_00015		PRO_0000322714
Regions
	DNA binding	48 – 68	21	H-T-H motif By similarity
Sites
	Active site	173	1	For autocatalytic cleavage activity By similarity
	Active site	210	1	For autocatalytic cleavage activity By similarity
	Site	138 – 139	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JUZ2-1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 036231710BB094B6	FASTA	249	26,884
	10 20 30 40 50 60 MAAQATGGRA TQRSQQSPAK PKGLTVRQKK ILETIQRSVN DNGYPPSMRE IGDTVGLASL 70 80 90 100 110 120 SSVTHQLSQL EKLGYLRRDP KRPRAMEVLM PLTLDEGTAK ISGVEKPARL RTIGGLAVSE 130 140 150 160 170 180 LATATDTAMV PLVGRIAAGG PILADQVVED VMPLPRQLVG HGELFMLKVT GDSMIDAAIC 190 200 210 220 230 240 DGDWVVVRRQ SDAVNGDIVA ALLDDEATVK TFRQRDGHTW LLPQNTQYEP ILGDHANIMG KVVSVFRSL

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000454 Genomic DNA. Translation: ABK02862.1. Different initiation.
RefSeq	YP_830962.1.
3D structure databases
SMR	A0JUZ2. Positions 130-248.
ModBase	Search...
Protein-protein interaction databases
STRING	A0JUZ2.
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	4445999.
GenomeReviews	Gene locus Arth_1468 in contig CP000454_GR.
KEGG	art:Arth_1468.
TIGR	Arth_1468.
Phylogenomic databases
eggNOG	COG1974.
HOGENOM	HBG679610.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_ARTS2

Accession

Primary (citable) accession number: A0JUZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	February 9, 2010
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)