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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Arthrobacter sp. (strain FB24)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 32ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:Arth_1463
OrganismiArthrobacter sp. (strain FB24)
Taxonomic identifieri290399 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeArthrobacter
Proteomesi
  • UP000000754 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003770701 – 312tRNA dimethylallyltransferaseAdd BLAST312

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116Interaction with substrate tRNAUniRule annotation1
Sitei137Interaction with substrate tRNAUniRule annotation1

Protein-protein interaction databases

STRINGi290399.Arth_1463.

Structurei

3D structure databases

ProteinModelPortaliA0JUY7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 32Substrate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiTWFRNQT.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0JUY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPQEHSGP TTQPRGVPPV IAVVGPTGSG KSDLAVALAL ELDGEVINAD
60 70 80 90 100
AMQFYRGMDI GTAKITAEER RGVPHHLLDT LDVTEEASVS DFQWEARATI
110 120 130 140 150
AEIHGRGKRA ILAGGSGLYV RAALDVLEFP GTDPGIRRRL EEELDDAGLA
160 170 180 190 200
TLRRRLEAVD PVSAARLGDA RRVVRALEVF ELTGRAFSSF MPVREYYQPA
210 220 230 240 250
VQIGLEVDRE LLKDRLAQRV HGMVANGLQA EVQRLDAAGL RHGKTASRAL
260 270 280 290 300
GYSQFLKVLD GEWDTGQATE DTIVATRKFA RRQLTWFRAD PRIHWFDWQD
310
PDLVAKALAV CR
Length:312
Mass (Da):34,311
Last modified:December 12, 2006 - v1
Checksum:i4DC3A2770EB0E521
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK02857.1.
RefSeqiWP_011691324.1. NC_008541.1.

Genome annotation databases

EnsemblBacteriaiABK02857; ABK02857; Arth_1463.
KEGGiart:Arth_1463.
PATRICi20998219. VBIArtSp72239_1819.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK02857.1.
RefSeqiWP_011691324.1. NC_008541.1.

3D structure databases

ProteinModelPortaliA0JUY7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290399.Arth_1463.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK02857; ABK02857; Arth_1463.
KEGGiart:Arth_1463.
PATRICi20998219. VBIArtSp72239_1819.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiTWFRNQT.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_ARTS2
AccessioniPrimary (citable) accession number: A0JUY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.