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Protein

Proline--tRNA ligase

Gene

proS

Organism
Arthrobacter sp. (strain FB24)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciASP290399:GHIF-1439-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:Arth_1407
OrganismiArthrobacter sp. (strain FB24)
Taxonomic identifieri290399 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeArthrobacter
Proteomesi
  • UP000000754 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 603603Proline--tRNA ligasePRO_0000288310Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi290399.Arth_1407.

Structurei

3D structure databases

ProteinModelPortaliA0JUT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C90. Bacteria.
COG0442. LUCA.
HOGENOMiHOG000076893.
KOiK01881.
OMAiIQPAELW.
OrthoDBiPOG091H0290.

Family and domain databases

CDDicd00779. ProRS_core_prok. 1 hit.
Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR033730. ProRS_core_prok.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JUT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLRLSKLFL RTLREDPADA EVASHRLLVR AGYIRRAAPG IYTWLPLGLS
60 70 80 90 100
VLRKVEKVIR EEMAAIGAQE VHFPALLPKE PYEATNRWTE YGEGIFRLKD
110 120 130 140 150
RKGGDYLLAP THEEMFTLLV KDLYSSYKDL PLSIYQIQNK YRDEARPRAG
160 170 180 190 200
LLRGREFIMK DSYSFDVDDA GLDASYNAHR AAYLKIFERL GLEVIPVAAT
210 220 230 240 250
AGAMGGSRSE EFLHPTEIGE DTFVRSAGGY AANVEAVTTV VPAEIDFSNA
260 270 280 290 300
PAAEIRDTPN TPTIDTLVDA ANQLVPRDEN DGGAWTAADT LKNVVLAVTL
310 320 330 340 350
PTGERQIVVI GVPGDRGVDL KRVEANIGAY LPVAGEITVE AAGEEDLARN
360 370 380 390 400
PLIVRGYLGP GMSLGTPLLG LEGAAKLLYL VDPRVVKGTA WVTGANMAGK
410 420 430 440 450
HVFGLVAGRD FGWDGVIECT EVRAGDEAPD GSGPLETARG IEMGHIFQLG
460 470 480 490 500
RKYAEALELK VLDQNGKQVV VTMGSYGVGV TRAVAALAES NHDAKGLVWP
510 520 530 540 550
RAVAPADVHV VAVGRGEEIF AAAEQLSLEL EAAGLEVIYD DRPKVSPGVK
560 570 580 590 600
FGDAELIGVP TILAVGRGLV DGVVEIKDRR SGEAENVAVE KAVDYVVNAV

RSK
Length:603
Mass (Da):64,928
Last modified:December 12, 2006 - v1
Checksum:i3DCAFF181CC2E798
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK02801.1.
RefSeqiWP_011691268.1. NC_008541.1.

Genome annotation databases

EnsemblBacteriaiABK02801; ABK02801; Arth_1407.
KEGGiart:Arth_1407.
PATRICi20998105. VBIArtSp72239_1762.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK02801.1.
RefSeqiWP_011691268.1. NC_008541.1.

3D structure databases

ProteinModelPortaliA0JUT1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290399.Arth_1407.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK02801; ABK02801; Arth_1407.
KEGGiart:Arth_1407.
PATRICi20998105. VBIArtSp72239_1762.

Phylogenomic databases

eggNOGiENOG4105C90. Bacteria.
COG0442. LUCA.
HOGENOMiHOG000076893.
KOiK01881.
OMAiIQPAELW.
OrthoDBiPOG091H0290.

Enzyme and pathway databases

BioCyciASP290399:GHIF-1439-MONOMER.

Family and domain databases

CDDicd00779. ProRS_core_prok. 1 hit.
Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR033730. ProRS_core_prok.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYP_ARTS2
AccessioniPrimary (citable) accession number: A0JUT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: December 12, 2006
Last modified: September 7, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.