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Protein

Proline--tRNA ligase

Gene

proS

Organism
Arthrobacter sp. (strain FB24)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:Arth_1407
OrganismiArthrobacter sp. (strain FB24)
Taxonomic identifieri290399 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeArthrobacter
Proteomesi
  • UP000000754 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002883101 – 603Proline--tRNA ligaseAdd BLAST603

Proteomic databases

PRIDEiA0JUT1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi290399.Arth_1407

Structurei

3D structure databases

ProteinModelPortaliA0JUT1
SMRiA0JUT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C90 Bacteria
COG0442 LUCA
HOGENOMiHOG000076893
KOiK01881
OMAiIQPAELW
OrthoDBiPOG091H0290

Family and domain databases

CDDicd00779 ProRS_core_prok, 1 hit
Gene3Di3.40.50.800, 2 hits
3.90.960.10, 1 hit
HAMAPiMF_01569 Pro_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR002316 Pro-tRNA-ligase_IIa
IPR004500 Pro-tRNA-synth_IIa_bac-type
IPR023717 Pro-tRNA-Synthase_IIa_type1
IPR033730 ProRS_core_prok
IPR036754 YbaK/aa-tRNA-synt-asso_dom_sf
IPR007214 YbaK/aa-tRNA-synth-assoc-dom
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF04073 tRNA_edit, 1 hit
PRINTSiPR01046 TRNASYNTHPRO
TIGRFAMsiTIGR00409 proS_fam_II, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

A0JUT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLRLSKLFL RTLREDPADA EVASHRLLVR AGYIRRAAPG IYTWLPLGLS
60 70 80 90 100
VLRKVEKVIR EEMAAIGAQE VHFPALLPKE PYEATNRWTE YGEGIFRLKD
110 120 130 140 150
RKGGDYLLAP THEEMFTLLV KDLYSSYKDL PLSIYQIQNK YRDEARPRAG
160 170 180 190 200
LLRGREFIMK DSYSFDVDDA GLDASYNAHR AAYLKIFERL GLEVIPVAAT
210 220 230 240 250
AGAMGGSRSE EFLHPTEIGE DTFVRSAGGY AANVEAVTTV VPAEIDFSNA
260 270 280 290 300
PAAEIRDTPN TPTIDTLVDA ANQLVPRDEN DGGAWTAADT LKNVVLAVTL
310 320 330 340 350
PTGERQIVVI GVPGDRGVDL KRVEANIGAY LPVAGEITVE AAGEEDLARN
360 370 380 390 400
PLIVRGYLGP GMSLGTPLLG LEGAAKLLYL VDPRVVKGTA WVTGANMAGK
410 420 430 440 450
HVFGLVAGRD FGWDGVIECT EVRAGDEAPD GSGPLETARG IEMGHIFQLG
460 470 480 490 500
RKYAEALELK VLDQNGKQVV VTMGSYGVGV TRAVAALAES NHDAKGLVWP
510 520 530 540 550
RAVAPADVHV VAVGRGEEIF AAAEQLSLEL EAAGLEVIYD DRPKVSPGVK
560 570 580 590 600
FGDAELIGVP TILAVGRGLV DGVVEIKDRR SGEAENVAVE KAVDYVVNAV

RSK
Length:603
Mass (Da):64,928
Last modified:December 12, 2006 - v1
Checksum:i3DCAFF181CC2E798
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA Translation: ABK02801.1
RefSeqiWP_011691268.1, NC_008541.1

Genome annotation databases

EnsemblBacteriaiABK02801; ABK02801; Arth_1407
KEGGiart:Arth_1407

Similar proteinsi

Entry informationi

Entry nameiSYP_ARTS2
AccessioniPrimary (citable) accession number: A0JUT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: December 12, 2006
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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