Reviewed, UniProtKB/Swiss-Prot A0JUH2 (DNLJ_ARTS2)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.2
Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Ordered Locus Names:	Arth_1298

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	790 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity.
Catalytic activity	NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588
Cofactor	Magnesium or manganese By similarity.
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (NAD+) activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 790	790	DNA ligase HAMAP MF_01588		PRO_0000313117
Regions
	Domain	679 – 768	90	BRCT
	Nucleotide binding	79 – 83	5	NAD By similarity
	Nucleotide binding	128 – 129	2	NAD By similarity
Sites
	Active site	166	1	N6-AMP-lysine intermediate By similarity
	Metal binding	461	1	Zinc By similarity
	Metal binding	464	1	Zinc By similarity
	Metal binding	480	1	Zinc By similarity
	Metal binding	486	1	Zinc By similarity
	Binding site	164	1	NAD By similarity
	Binding site	187	1	NAD By similarity
	Binding site	224	1	NAD By similarity
	Binding site	340	1	NAD By similarity
	Binding site	364	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JUH2-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 8771FABBDF3747E2	FASTA	790	85,847
	10 20 30 40 50 60 MRLEPDNVGD LGQDRSVSTG NTAADTDTQS MTGAGTREAD TASERVPSGA VREEYENLVE 70 80 90 100 110 120 EVRKHRFAYY QEDAPIISDA EFDELFRRLE TIEAMHPELV ANDSPTQEVG GEVSAAFAAV 130 140 150 160 170 180 EHLQRMYSLE DVFSLEELES WIAKAEASVA KLGNGTAGPA WLTELKIDGL AVNLLYRDGK 190 200 210 220 230 240 LVRAATRGDG YTGEDITHNV LTIKEIPQQL TGGNYPAEME VRGEVFIPSK AFAEYNEALI 250 260 270 280 290 300 EAGKAPLANP RNAAAGSLRQ KDPAETAKRP LRMYVHGIGA REGLRTVSQS DTYRQLAEWG 310 320 330 340 350 360 LPTSPYFEVL GSLKDILDFI KRYGDKRHSL THEIDGIVVK VDDFATQRAL GYTSRVPRWA 370 380 390 400 410 420 VAYKYPPEEV HTKLLDIAVN VGRTGRVTPF GIMEPVKVAG STVGMATLHN QDVVKAKGVK 430 440 450 460 470 480 IGDIVVLRKA GDVIPEIVGP VLALRDQQDP PVRDFVMPTE CPACGTPLAP GKEGDVDIRC 490 500 510 520 530 540 PNSRSCPAQL RERVFHVASR GAFDIEALGW EAAIALTQPA EPVTPPLTSE ANLFRLTRED 550 560 570 580 590 600 LADVRIRREK RSKGVATGEF ELVPYFYTKG TAKSPSKPTA TTEKLFTELE KAKSQPLWRV 610 620 630 640 650 660 LVALSIRHVG PRASRALATA FGSMDAIREA SEEELAHVDG VGPVIAAALK EWFAEDWHRE 670 680 690 700 710 720 IVDTWAEDGV RMEDEQDEST PRTLEGLTVV VTGSLEGFSR DEAKEAILIR GGKASGSVSK 730 740 750 760 770 780 NTSYVVAGEN AGTKLDKAEQ LGVPVLDEDG FRALLANGPA ASADSADASA DDDDAGVGDR 790 PEDHQEEPAE

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000454 Genomic DNA. Translation: ABK02692.1.
RefSeq	YP_830792.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0JUH2.
Genome annotation databases
GeneID	4446222.
GenomeReviews	Gene locus Arth_1298 in contig CP000454_GR.
KEGG	art:Arth_1298.
TIGR	Arth_1298.
Phylogenomic databases
OMA	YKFPAQE.
Family and domain databases
HAMAP	MF_01588. [Tree]
InterPro	IPR001357. BRCT. IPR018239. DNA_ligase_AS. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR005220. DUF388_OB-fold. IPR000445. HhH_motif. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR004149. Znf_DNAligase_C4. [Graphical view]
Gene3D	G3DSA:2.40.50.200. DNA_ligase_OB. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. PF03119. DNA_ligase_ZBD. 1 hit. PF00633. HHH. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
ProDom	PD003944. DNAligase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00292. BRCT. 1 hit. SM00278. HhH1. 1 hit. SM00532. LIGANc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00575. dnlj. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLJ_ARTS2

Accession

Primary (citable) accession number: A0JUH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)