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A0JTA3 (BIOB_ARTS2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Arth_0875
OrganismArthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]
Taxonomic identifier290399 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Biotin synthase HAMAP-Rule MF_01694
PRO_0000381210

Sites

Metal binding921Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding961Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding991Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1351Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1681Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2271Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2971Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A0JTA3 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 8FC6B0307FA8D97C

FASTA35638,689
        10         20         30         40         50         60 
MTIQANVPTG DETSDEASRQ TSNEASSEIL ETARQQVLEN GVGLTQAQLE EFLRLPDEAL 

        70         80         90        100        110        120 
PAALQLAHEV RLKHCGEDVE VEGIISIKTG GCPEDCHFCS QSGLFDSPVR GVWLDIPELV 

       130        140        150        160        170        180 
KAAKETAATG ATEFCIVAAV RCPDIKLMNQ IKFAIDRINE AVDINIACSL GMLTQRQVDQ 

       190        200        210        220        230        240 
LAEWGVHRYN HNLETARSYF PEVVTTHSYE ERLETCNMVK AAGMELCCGA LIGMGETLEQ 

       250        260        270        280        290        300 
RAELAAQLAA LEPHEVPLNF LNPRPGTPLE NQGIMDGKDA LRAIAAFRLA MPRTVLRYAG 

       310        320        330        340        350 
GRELTLGDLG TREGLLGGIN AVIVGNYLTT LGRPATADLS LLVELNMPIK ELQKTL 

« Hide

References

[1]"Complete sequence of chromosome 1 of Arthrobacter sp. FB24."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. expand/collapse author list , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FB24.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000454 Genomic DNA. Translation: ABK02273.1.
RefSeqYP_830373.1. NC_008541.1.

3D structure databases

ProteinModelPortalA0JTA3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290399.Arth_0875.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK02273; ABK02273; Arth_0875.
GeneID4446608.
KEGGart:Arth_0875.
PATRIC20997007. VBIArtSp72239_1221.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMATCENTLR.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycASP290399:GHIF-898-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_ARTS2
AccessionPrimary (citable) accession number: A0JTA3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 12, 2006
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways