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A0JTA3

- BIOB_ARTS2

UniProt

A0JTA3 - BIOB_ARTS2

Protein

Biotin synthase

Gene

bioB

Organism
Arthrobacter sp. (strain FB24)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi96 – 961Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi135 – 1351Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi168 – 1681Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi227 – 2271Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi297 – 2971Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciASP290399:GHIF-898-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Arth_0875
    OrganismiArthrobacter sp. (strain FB24)
    Taxonomic identifieri290399 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
    ProteomesiUP000000754: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356Biotin synthasePRO_0000381210Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi290399.Arth_0875.

    Structurei

    3D structure databases

    ProteinModelPortaliA0JTA3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiTCENTLR.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0JTA3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTIQANVPTG DETSDEASRQ TSNEASSEIL ETARQQVLEN GVGLTQAQLE    50
    EFLRLPDEAL PAALQLAHEV RLKHCGEDVE VEGIISIKTG GCPEDCHFCS 100
    QSGLFDSPVR GVWLDIPELV KAAKETAATG ATEFCIVAAV RCPDIKLMNQ 150
    IKFAIDRINE AVDINIACSL GMLTQRQVDQ LAEWGVHRYN HNLETARSYF 200
    PEVVTTHSYE ERLETCNMVK AAGMELCCGA LIGMGETLEQ RAELAAQLAA 250
    LEPHEVPLNF LNPRPGTPLE NQGIMDGKDA LRAIAAFRLA MPRTVLRYAG 300
    GRELTLGDLG TREGLLGGIN AVIVGNYLTT LGRPATADLS LLVELNMPIK 350
    ELQKTL 356
    Length:356
    Mass (Da):38,689
    Last modified:December 12, 2006 - v1
    Checksum:i8FC6B0307FA8D97C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000454 Genomic DNA. Translation: ABK02273.1.
    RefSeqiWP_011690740.1. NC_008541.1.
    YP_830373.1. NC_008541.1.

    Genome annotation databases

    EnsemblBacteriaiABK02273; ABK02273; Arth_0875.
    GeneIDi4446608.
    KEGGiart:Arth_0875.
    PATRICi20997007. VBIArtSp72239_1221.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000454 Genomic DNA. Translation: ABK02273.1 .
    RefSeqi WP_011690740.1. NC_008541.1.
    YP_830373.1. NC_008541.1.

    3D structure databases

    ProteinModelPortali A0JTA3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290399.Arth_0875.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK02273 ; ABK02273 ; Arth_0875 .
    GeneIDi 4446608.
    KEGGi art:Arth_0875.
    PATRICi 20997007. VBIArtSp72239_1221.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi TCENTLR.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci ASP290399:GHIF-898-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FB24.

    Entry informationi

    Entry nameiBIOB_ARTS2
    AccessioniPrimary (citable) accession number: A0JTA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3