Reviewed, UniProtKB/Swiss-Prot A0JSV8 (SYC_ARTS2)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Arth_0730

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_1000006562
Regions
	Motif	29 – 39	11	"HIGH" region HAMAP MF_00041
	Motif	281 – 285	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	27	1	Zinc By similarity
	Metal binding	225	1	Zinc By similarity
	Metal binding	250	1	Zinc By similarity
	Metal binding	254	1	Zinc By similarity
	Binding site	284	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JSV8-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: C7CCCB88B4A594B2	FASTA	487	53,229
	10 20 30 40 50 60 MRFYDTASAE VRDFVPLIPG KVSLYYCGAT VQGLPHVGHI RSAIAFDQLT RWLEYRGFRV 70 80 90 100 110 120 TVVRNVTDID DKILAKSALS FEPDFEEGPD DVREEEWWAL AYRYEQAFLK AYDTLGVSRP 130 140 150 160 170 180 TYEPRATGHI PEMHALIQGL IDRGHAYPAL DDSGDVYFDV RSWNRYGSLT RQKIDDMQGA 190 200 210 220 230 240 PDADPRGKKD PRDFALWKGH KEGEPTTASW VSPWGTGRPG WHLECSAMVT KYLGTEFDIH 250 260 270 280 290 300 GGGLDLRFPH HENEMAQSQA AGHAFANFWM HNGMVTYQGE KMSKSIGNTV SPAEMLELAS 310 320 330 340 350 360 PRVVRYYLGQ AQYRSVLDYQ PTSLQEAAAA VERIDGFIAR AVRTLSGSVV SGAAELLFSS 370 380 390 400 410 420 HGTVPDAFAA AMDDDLNVPQ ALAALHDTVR AGNTALTAGD NSAARTALHS VTDMLRVLGL 430 440 450 460 470 480 NDVAAPARDE QADTALGVLV EAQLGARAQA RATKDWAASD AIRDTLAAAG VVVEDGPDGA SWSLKRG

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000454 Genomic DNA. Translation: ABK02128.1.
RefSeq	YP_830228.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0JSV8.
Genome annotation databases
GeneID	4446764.
GenomeReviews	Gene locus Arth_0730 in contig CP000454_GR.
KEGG	art:Arth_0730.
TIGR	Arth_0730.
Phylogenomic databases
OMA	VLWKAAK.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ARTS2

Accession

Primary (citable) accession number: A0JSV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)