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A0JSP6 (PCKG_ARTS2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:Arth_0667
OrganismArthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]
Taxonomic identifier290399 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length611 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 611611Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000072370

Regions

Nucleotide binding274 – 2796GTP By similarity
Nucleotide binding517 – 5204GTP By similarity
Region389 – 3913Substrate binding By similarity

Sites

Active site2751 By similarity
Metal binding2311Manganese By similarity
Metal binding2511Manganese; via tele nitrogen By similarity
Metal binding2981Manganese By similarity
Binding site821Substrate By similarity
Binding site2241Substrate; via amide nitrogen By similarity
Binding site2311Substrate By similarity
Binding site2731Substrate By similarity
Binding site3911GTP By similarity
Binding site4221GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0JSP6 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 13FC3189846C263D

FASTA61167,176
        10         20         30         40         50         60 
MGDLARLPLL EKAPTTHAGL LAWVEEVAEL TQPDRIHWVD GTEEEYTRLT GELVEAGTLT 

        70         80         90        100        110        120 
RLNPELFPNS FAAFSDPADV ARVEEQTFIC SENQRDAGFT NNWMAPAEMK QKLRGLFAGS 

       130        140        150        160        170        180 
MRGRTMYVIP FVMGHLDAED PKFGVEITDS AYVVASMRIM ANIGTEVLDK ITATNAFFVP 

       190        200        210        220        230        240 
ALHSLGAPLA PGQADVAWPC NPDKWIVHFP EERSIWSFGS GYGGNALLGK KCYALRIASV 

       250        260        270        280        290        300 
MARDEGWLAE HMLILKLTSP EKKSYYMSAA FPSACGKTNL ALLDPTIEGW EVETLGDDIT 

       310        320        330        340        350        360 
WMRIGKEGEL RATNPEAGLF GVAPGTGWGT NPNAMRAIAK GHSIFTNVAL TDDGGVWWEG 

       370        380        390        400        410        420 
MTEETPAHLT DWQGNSWTPD SDKPAAHPNS RFCTPISQID MLAEEYYSPE GVELSAILFG 

       430        440        450        460        470        480 
GRRKTTVPLV TQARSWTNGI FMGSTLSSET TAAAAGQVGV LRRDPMAMLP FIGYDAGDYL 

       490        500        510        520        530        540 
KHWISVSGKA NPERLPHIFL VNWFRRTADG DFAWPGFGDN ARVLKWAIER IEGKADAIET 

       550        560        570        580        590        600 
PIGFVPAGHA LDLTGLDLTH AHVEDAVRVD REEWDAELAS IEEWYAKFGD SLPEALRAEL 

       610 
DALKERMADH S 

« Hide

References

[1]"Complete sequence of chromosome 1 of Arthrobacter sp. FB24."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. expand/collapse author list , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FB24.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000454 Genomic DNA. Translation: ABK02066.1.
RefSeqYP_830166.1. NC_008541.1.

3D structure databases

ProteinModelPortalA0JSP6.
SMRA0JSP6. Positions 13-608.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290399.Arth_0667.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK02066; ABK02066; Arth_0667.
GeneID4446848.
KEGGart:Arth_0667.
PATRIC20996579. VBIArtSp72239_1015.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAWMRFGED.
OrthoDBEOG6MPWQS.
ProtClustDBPRK04210.

Enzyme and pathway databases

BioCycASP290399:GHIF-682-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_ARTS2
AccessionPrimary (citable) accession number: A0JSP6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways