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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Arthrobacter sp. (strain FB24)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82SubstrateUniRule annotation1
Metal bindingi231ManganeseUniRule annotation1
Metal bindingi251Manganese; via tele nitrogenUniRule annotation1
Binding sitei273SubstrateUniRule annotation1
Active sitei275UniRule annotation1
Metal bindingi298ManganeseUniRule annotation1
Binding sitei391GTPUniRule annotation1
Binding sitei422GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi274 – 279GTPUniRule annotation6
Nucleotide bindingi517 – 520GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Arth_0667
OrganismiArthrobacter sp. (strain FB24)
Taxonomic identifieri290399 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeArthrobacter
Proteomesi
  • UP000000754 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000723701 – 611Phosphoenolpyruvate carboxykinase [GTP]Add BLAST611

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi290399.Arth_0667.

Structurei

3D structure databases

ProteinModelPortaliA0JSP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni222 – 224Substrate bindingUniRule annotation3
Regioni389 – 391Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JSP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDLARLPLL EKAPTTHAGL LAWVEEVAEL TQPDRIHWVD GTEEEYTRLT
60 70 80 90 100
GELVEAGTLT RLNPELFPNS FAAFSDPADV ARVEEQTFIC SENQRDAGFT
110 120 130 140 150
NNWMAPAEMK QKLRGLFAGS MRGRTMYVIP FVMGHLDAED PKFGVEITDS
160 170 180 190 200
AYVVASMRIM ANIGTEVLDK ITATNAFFVP ALHSLGAPLA PGQADVAWPC
210 220 230 240 250
NPDKWIVHFP EERSIWSFGS GYGGNALLGK KCYALRIASV MARDEGWLAE
260 270 280 290 300
HMLILKLTSP EKKSYYMSAA FPSACGKTNL ALLDPTIEGW EVETLGDDIT
310 320 330 340 350
WMRIGKEGEL RATNPEAGLF GVAPGTGWGT NPNAMRAIAK GHSIFTNVAL
360 370 380 390 400
TDDGGVWWEG MTEETPAHLT DWQGNSWTPD SDKPAAHPNS RFCTPISQID
410 420 430 440 450
MLAEEYYSPE GVELSAILFG GRRKTTVPLV TQARSWTNGI FMGSTLSSET
460 470 480 490 500
TAAAAGQVGV LRRDPMAMLP FIGYDAGDYL KHWISVSGKA NPERLPHIFL
510 520 530 540 550
VNWFRRTADG DFAWPGFGDN ARVLKWAIER IEGKADAIET PIGFVPAGHA
560 570 580 590 600
LDLTGLDLTH AHVEDAVRVD REEWDAELAS IEEWYAKFGD SLPEALRAEL
610
DALKERMADH S
Length:611
Mass (Da):67,176
Last modified:December 12, 2006 - v1
Checksum:i13FC3189846C263D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK02066.1.
RefSeqiWP_011690534.1. NC_008541.1.

Genome annotation databases

EnsemblBacteriaiABK02066; ABK02066; Arth_0667.
KEGGiart:Arth_0667.
PATRICi20996579. VBIArtSp72239_1015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000454 Genomic DNA. Translation: ABK02066.1.
RefSeqiWP_011690534.1. NC_008541.1.

3D structure databases

ProteinModelPortaliA0JSP6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290399.Arth_0667.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK02066; ABK02066; Arth_0667.
KEGGiart:Arth_0667.
PATRICi20996579. VBIArtSp72239_1015.

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_ARTS2
AccessioniPrimary (citable) accession number: A0JSP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.