Reviewed, UniProtKB/Swiss-Prot A0JRH4 (URE1_ARTS2)

Names and origin

Protein names

Recommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha

Gene names

Name:	ureC
Ordered Locus Names:	Arth_0243

Organism

Arthrobacter sp. (strain FB24) [Complete proteome] [HAMAP]

Taxonomic identifier

290399 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity.
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Urease subunit alpha HAMAP MF_01953		PRO_1000070647
Regions
	Domain	132 – 576	445	Urease
Sites
	Active site	323	1	Proton donor By similarity
	Metal binding	137	1	Nickel 2 By similarity
	Metal binding	139	1	Nickel 2 By similarity
	Metal binding	220	1	Nickel 1; via carbamate group By similarity
	Metal binding	220	1	Nickel 2; via carbamate group By similarity
	Metal binding	249	1	Nickel 1 By similarity
	Metal binding	275	1	Nickel 1 By similarity
	Metal binding	363	1	Nickel 2 By similarity
	Binding site	222	1	Substrate By similarity
Amino acid modifications
	Modified residue	220	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JRH4-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 58BC841B6AE59E84	FASTA	576	61,668
	10 20 30 40 50 60 MSFELTRRQY ADLYGPTTGD SIRLADTELF LEIEKDLTVY GEEVVFGGGK VIRDGMGQNG 70 80 90 100 110 120 QVTRDEDVPD TVITNAVILD YTGIYKADVA IRDGHIIKIG KAGNPQITDG VDIVIGASTE 130 140 150 160 170 180 IIAGERKILT AGGVDTHIHF ISPDQVPTAL TSGVTTMVGG GTGPAEGTKA TTVTPGKWHI 190 200 210 220 230 240 QRMLQATEGM PINIGLFGKG HASAVEPLAE QIRAGAIGLK VHEDWGSTTS SIDNSLKVAD 250 260 270 280 290 300 EYDVQVAIHT DTLNECGFVE DTIRAIGGRV IHTFHTEGAG GGHAPDIIKI AGLPNVLPAS 310 320 330 340 350 360 TNPTLPYTRN TIEEHLDMLM VCHHLNPDIP EDVAFADSRI RAETIAAEDV LQDLGIFAIT 370 380 390 400 410 420 SSDSQAMGRV GEVITRTWQV ADKMKKQRGV LKDPAGGAHG SEDSDNFRLK RYVAKYTINA 430 440 450 460 470 480 AIAQGMADSI GSVEEGKFAD LVLWDPAFFG VKPELVLKGG QIAYALMGDA NASIPTPQPR 490 500 510 520 530 540 TMRPMFAAFG KAVQQSSITF LSQAAIDAGV PEELGLQKVI RAVSGIRSLS KADLKYNDAT 550 560 570 PDIQVDPETY KVTVDGEDVT CEPADVLPMA QRYFLF

References

[1]

"Complete sequence of chromosome 1 of Arthrobacter sp. FB24." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Thompson S., Brettin T., Bruce D., Han C., Tapia R., Gilna P. , Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Beasley F., Chen W., Jerke K., Nakatsu C.H., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000454 Genomic DNA. Translation: ABK01644.1.
RefSeq	YP_829744.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0JRH4.
Genome annotation databases
GeneID	4447299.
GenomeReviews	Gene locus Arth_0243 in contig CP000454_GR.
KEGG	art:Arth_0243.
TIGR	Arth_0243.
Phylogenomic databases
OMA	SHIHFIC.
Family and domain databases
HAMAP	MF_01953. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017952. Urease_asu_core. IPR017950. Urease_asu_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
TIGRFAMs	TIGR01792. urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URE1_ARTS2

Accession

Primary (citable) accession number: A0JRH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)