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Protein

Tripartite motif-containing protein 72

Gene

Trim72

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca2+-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 5744RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri81 – 12242B box-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 72
Alternative name(s):
Mitsugumin-53
Short name:
Mg53
Gene namesi
Name:Trim72By similarity
Synonyms:Mg53
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1562778. Trim72.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Tripartite motif-containing protein 72PRO_0000278133Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei144 – 1441S-nitrosocysteineBy similarity
Disulfide bondi242 – 242InterchainBy similarity
Modified residuei255 – 2551PhosphoserineCombined sources

Post-translational modificationi

Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.By similarity
S-nitrosylation at Cys-144 stabilizes TRIM72 and protects against oxidation-induced protein degradation and cell death.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiA0JPQ4.
PeptideAtlasiA0JPQ4.
PRIDEiA0JPQ4.

PTM databases

iPTMnetiA0JPQ4.
PhosphoSiteiA0JPQ4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000022099.
GenevisibleiA0JPQ4. RN.

Interactioni

Subunit structurei

Homooligomer; disulfide-linked. Interacts with DYSF and CAV3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000030976.

Structurei

3D structure databases

ProteinModelPortaliA0JPQ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini271 – 475205B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili122 – 16948Sequence analysisAdd
BLAST
Coiled coili204 – 23229Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Sequence analysis
Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 5744RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri81 – 12242B box-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000234133.
HOVERGENiHBG098570.
InParanoidiA0JPQ4.
KOiK12036.
OMAiPCPCCQA.
OrthoDBiEOG091G06AH.
PhylomeDBiA0JPQ4.
TreeFamiTF342569.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JPQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTAPGLLRQ ELSCPLCLQL FDAPVTAECG HSFCRACLIR VAGEPADDGT
60 70 80 90 100
VACPCCQAST RPQALSTNLQ LARLVEGLAQ VPQGHCEEHL DPLSIYCEQD
110 120 130 140 150
RTLVCGVCAS LGSHRGHRLL PAAEAHARLK TQLPQQKAQL QEACMRKEKS
160 170 180 190 200
VAVLEHQLVE VEETVRQFRG AVGEQLGKMR MFLAALESSL DREAERVRGE
210 220 230 240 250
AGVALRRELS SLNSYLEQLR QMEKVLEEVA DKPQTEFLMK FCLVTSRLQK
260 270 280 290 300
ILSESPPPAR LDIQLPVISD DFKFQVWKKM FRALMPELEE LTFDPSSAHP
310 320 330 340 350
SLVVSASGRR VECSEQKAPP AGEDTCQFDK TVAVVAKQLL SQGEHYWEVE
360 370 380 390 400
VGDKPRWALG VMAADASRRG RLHAVPSQGL WLLGLRDGKI LEAHVEAKEP
410 420 430 440 450
RALRTPERPP ARIGLYLSFA DGVLTFYDAS NTDALTPLFS FHERLPGPVY
460 470
PMFDVCWHDK GKNSQPLLLV GPDSEQA
Length:477
Mass (Da):52,832
Last modified:December 12, 2006 - v1
Checksum:i7D095A96969D69B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC127539 mRNA. Translation: AAI27540.1.
RefSeqiNP_001071143.1. NM_001077675.1.
UniGeneiRn.23565.

Genome annotation databases

EnsembliENSRNOT00000029994; ENSRNOP00000030976; ENSRNOG00000022099.
GeneIDi365377.
KEGGirno:365377.
UCSCiRGD:1562778. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC127539 mRNA. Translation: AAI27540.1.
RefSeqiNP_001071143.1. NM_001077675.1.
UniGeneiRn.23565.

3D structure databases

ProteinModelPortaliA0JPQ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000030976.

PTM databases

iPTMnetiA0JPQ4.
PhosphoSiteiA0JPQ4.

Proteomic databases

PaxDbiA0JPQ4.
PeptideAtlasiA0JPQ4.
PRIDEiA0JPQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029994; ENSRNOP00000030976; ENSRNOG00000022099.
GeneIDi365377.
KEGGirno:365377.
UCSCiRGD:1562778. rat.

Organism-specific databases

CTDi493829.
RGDi1562778. Trim72.

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000234133.
HOVERGENiHBG098570.
InParanoidiA0JPQ4.
KOiK12036.
OMAiPCPCCQA.
OrthoDBiEOG091G06AH.
PhylomeDBiA0JPQ4.
TreeFamiTF342569.

Enzyme and pathway databases

ReactomeiR-RNO-445355. Smooth Muscle Contraction.

Miscellaneous databases

PROiA0JPQ4.

Gene expression databases

BgeeiENSRNOG00000022099.
GenevisibleiA0JPQ4. RN.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRI72_RAT
AccessioniPrimary (citable) accession number: A0JPQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 12, 2006
Last modified: September 7, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.