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Protein

Tripartite motif-containing protein 72

Gene

Trim72

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca2+-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri14 – 57RING-typeAdd BLAST44
Zinc fingeri81 – 122B box-typeAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processExocytosis, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 72
Alternative name(s):
Mitsugumin-53
Short name:
Mg53
Gene namesi
Name:Trim72By similarity
Synonyms:Mg53
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1562778. Trim72.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002781331 – 477Tripartite motif-containing protein 72Add BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei144S-nitrosocysteineBy similarity1
Disulfide bondi242InterchainBy similarity
Modified residuei255PhosphoserineCombined sources1

Post-translational modificationi

Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.By similarity
S-nitrosylation at Cys-144 stabilizes TRIM72 and protects against oxidation-induced protein degradation and cell death.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiA0JPQ4.
PeptideAtlasiA0JPQ4.
PRIDEiA0JPQ4.

PTM databases

iPTMnetiA0JPQ4.
PhosphoSitePlusiA0JPQ4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000022099.
GenevisibleiA0JPQ4. RN.

Interactioni

Subunit structurei

Homooligomer; disulfide-linked. Interacts with DYSF and CAV3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000030976.

Structurei

3D structure databases

ProteinModelPortaliA0JPQ4.
SMRiA0JPQ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini271 – 475B30.2/SPRYAdd BLAST205

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili122 – 169Sequence analysisAdd BLAST48
Coiled coili204 – 232Sequence analysisAdd BLAST29

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Sequence analysis

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri14 – 57RING-typeAdd BLAST44
Zinc fingeri81 – 122B box-typeAdd BLAST42

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000234133.
HOVERGENiHBG098570.
InParanoidiA0JPQ4.
KOiK12036.
OMAiSDGEHYW.
OrthoDBiEOG091G06AH.
PhylomeDBiA0JPQ4.
TreeFamiTF342569.

Family and domain databases

CDDicd00021. BBOX. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001870. B30.2/SPRY.
IPR003879. Butyrophylin_SPRY.
IPR013320. ConA-like_dom_sf.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR035038. TRIM72.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PANTHERiPTHR24103:SF11. PTHR24103:SF11. 1 hit.
PfamiView protein in Pfam
PF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiView protein in SMART
SM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A0JPQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTAPGLLRQ ELSCPLCLQL FDAPVTAECG HSFCRACLIR VAGEPADDGT
60 70 80 90 100
VACPCCQAST RPQALSTNLQ LARLVEGLAQ VPQGHCEEHL DPLSIYCEQD
110 120 130 140 150
RTLVCGVCAS LGSHRGHRLL PAAEAHARLK TQLPQQKAQL QEACMRKEKS
160 170 180 190 200
VAVLEHQLVE VEETVRQFRG AVGEQLGKMR MFLAALESSL DREAERVRGE
210 220 230 240 250
AGVALRRELS SLNSYLEQLR QMEKVLEEVA DKPQTEFLMK FCLVTSRLQK
260 270 280 290 300
ILSESPPPAR LDIQLPVISD DFKFQVWKKM FRALMPELEE LTFDPSSAHP
310 320 330 340 350
SLVVSASGRR VECSEQKAPP AGEDTCQFDK TVAVVAKQLL SQGEHYWEVE
360 370 380 390 400
VGDKPRWALG VMAADASRRG RLHAVPSQGL WLLGLRDGKI LEAHVEAKEP
410 420 430 440 450
RALRTPERPP ARIGLYLSFA DGVLTFYDAS NTDALTPLFS FHERLPGPVY
460 470
PMFDVCWHDK GKNSQPLLLV GPDSEQA
Length:477
Mass (Da):52,832
Last modified:December 12, 2006 - v1
Checksum:i7D095A96969D69B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC127539 mRNA. Translation: AAI27540.1.
RefSeqiNP_001071143.1. NM_001077675.1.
UniGeneiRn.23565.

Genome annotation databases

EnsembliENSRNOT00000029994; ENSRNOP00000030976; ENSRNOG00000022099.
GeneIDi365377.
KEGGirno:365377.
UCSCiRGD:1562778. rat.

Similar proteinsi

Entry informationi

Entry nameiTRI72_RAT
AccessioniPrimary (citable) accession number: A0JPQ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 12, 2006
Last modified: November 22, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families