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Protein

Eukaryotic translation initiation factor 3 subunit J

Gene

Eif3j

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-RNO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-RNO-72649. Translation initiation complex formation.
R-RNO-72689. Formation of a pool of free 40S subunits.
R-RNO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-RNO-72702. Ribosomal scanning and start codon recognition.
R-RNO-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit JUniRule annotation
Short name:
eIF3jUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 1UniRule annotation
eIF-3-alphaUniRule annotation
eIF3 p35UniRule annotation
Gene namesi
Name:Eif3j
Synonyms:Eif3s1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1593338. Eif3j.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Eukaryotic translation initiation factor 3 subunit JPRO_0000329452Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121PhosphoserineCombined sources
Modified residuei14 – 141PhosphoserineUniRule annotationBy similarity
Modified residuei21 – 211PhosphoserineUniRule annotationBy similarity
Modified residuei110 – 1101PhosphothreonineCombined sources
Modified residuei128 – 1281PhosphoserineUniRule annotationBy similarity
Modified residuei255 – 2551PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA0JPM9.
PeptideAtlasiA0JPM9.
PRIDEiA0JPM9.

PTM databases

iPTMnetiA0JPM9.
PhosphoSiteiA0JPM9.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016459.
GenevisibleiA0JPM9. RN.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022437.

Structurei

3D structure databases

ProteinModelPortaliA0JPM9.
SMRiA0JPM9. Positions 143-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7070Sufficient for interaction with EIF3BUniRule annotationAdd
BLAST
Regioni244 – 25916Promotes stable association with the 40S ribosomeUniRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili71 – 13666UniRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 98Poly-Ala
Compositional biasi30 – 334Poly-Gly
Compositional biasi54 – 585Poly-Asp
Compositional biasi219 – 2257Poly-Lys

Sequence similaritiesi

Belongs to the eIF-3 subunit J family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4813. Eukaryota.
ENOG4111U5G. LUCA.
GeneTreeiENSGT00390000018400.
HOGENOMiHOG000238746.
HOVERGENiHBG066230.
InParanoidiA0JPM9.
KOiK03245.
OMAiSSREDFT.
OrthoDBiEOG091G141O.
PhylomeDBiA0JPM9.

Family and domain databases

Gene3Di1.10.246.60. 1 hit.
HAMAPiMF_03009. eIF3j. 1 hit.
InterProiIPR023194. eIF3-like_dom.
IPR013906. eIF3j.
[Graphical view]
PANTHERiPTHR21681. PTHR21681. 1 hit.
PfamiPF08597. eIF3_subunit. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JPM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAAAAAG DSDSWDADTF SMEDPVRKVA GGGTAGGDRW EGEDEDEDVK
60 70 80 90 100
DNWDDDDDEN KEEAEVKPEV KISEKKKIAE KIKEKERQQK KRQEEIKKRL
110 120 130 140 150
EEPEESKVLT PEEQLADKLR LKKLQEESDL ELAKETFGVN NTVYGIDAMN
160 170 180 190 200
PSSRDDFTEF GKLLKDKITQ YEKSLYYASF LEALVRDVCI SLEIDDLKKI
210 220 230 240 250
TNSLTVLCSE KQKQEKQSKA KKKKKGVVPG GGLKATMKDD LADYGGYDGG

YVQDYEDFM
Length:259
Mass (Da):29,187
Last modified:December 12, 2006 - v1
Checksum:i8815107BD8B8D210
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC127509 mRNA. Translation: AAI27510.1.
RefSeqiNP_001071138.1. NM_001077670.1.
UniGeneiRn.162681.

Genome annotation databases

EnsembliENSRNOT00000022437; ENSRNOP00000022437; ENSRNOG00000016459.
GeneIDi691947.
KEGGirno:691947.
UCSCiRGD:1593338. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC127509 mRNA. Translation: AAI27510.1.
RefSeqiNP_001071138.1. NM_001077670.1.
UniGeneiRn.162681.

3D structure databases

ProteinModelPortaliA0JPM9.
SMRiA0JPM9. Positions 143-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022437.

PTM databases

iPTMnetiA0JPM9.
PhosphoSiteiA0JPM9.

Proteomic databases

PaxDbiA0JPM9.
PeptideAtlasiA0JPM9.
PRIDEiA0JPM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022437; ENSRNOP00000022437; ENSRNOG00000016459.
GeneIDi691947.
KEGGirno:691947.
UCSCiRGD:1593338. rat.

Organism-specific databases

CTDi8669.
RGDi1593338. Eif3j.

Phylogenomic databases

eggNOGiKOG4813. Eukaryota.
ENOG4111U5G. LUCA.
GeneTreeiENSGT00390000018400.
HOGENOMiHOG000238746.
HOVERGENiHBG066230.
InParanoidiA0JPM9.
KOiK03245.
OMAiSSREDFT.
OrthoDBiEOG091G141O.
PhylomeDBiA0JPM9.

Enzyme and pathway databases

ReactomeiR-RNO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-RNO-72649. Translation initiation complex formation.
R-RNO-72689. Formation of a pool of free 40S subunits.
R-RNO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-RNO-72702. Ribosomal scanning and start codon recognition.
R-RNO-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

PROiA0JPM9.

Gene expression databases

BgeeiENSRNOG00000016459.
GenevisibleiA0JPM9. RN.

Family and domain databases

Gene3Di1.10.246.60. 1 hit.
HAMAPiMF_03009. eIF3j. 1 hit.
InterProiIPR023194. eIF3-like_dom.
IPR013906. eIF3j.
[Graphical view]
PANTHERiPTHR21681. PTHR21681. 1 hit.
PfamiPF08597. eIF3_subunit. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3J_RAT
AccessioniPrimary (citable) accession number: A0JPM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 12, 2006
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.