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Protein

60 kDa lysophospholipase

Gene

Aspg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
L-asparagine + H2O = L-aspartate + NH3.
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei19 – 191Acyl-ester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.47. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
60 kDa lysophospholipase (EC:3.1.1.5)
Including the following 2 domains:
L-asparaginase (EC:3.5.1.1)
Alternative name(s):
L-asparagine amidohydrolase
Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
Short name:
PAF acetylhydrolase
Gene namesi
Name:Aspg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2144822. Aspg.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 56456460 kDa lysophospholipasePRO_0000324164Add
BLAST

Proteomic databases

PaxDbiA0JNU3.
PRIDEiA0JNU3.

PTM databases

PhosphoSiteiA0JNU3.

Expressioni

Gene expression databases

BgeeiA0JNU3.
CleanExiMM_A530050D06RIK.
GenevisibleiA0JNU3. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078369.

Structurei

3D structure databases

ProteinModelPortaliA0JNU3.
SMRiA0JNU3. Positions 8-550.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 355347Asparaginase/glutaminasePROSITE-ProRule annotationAdd
BLAST
Repeati141 – 17030ANK 1Add
BLAST
Repeati396 – 42631ANK 2Add
BLAST
Repeati430 – 45930ANK 3Add
BLAST
Repeati463 – 49230ANK 4Add
BLAST
Repeati530 – 55930ANK 5Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 350310AsparaginaseAdd
BLAST
Regioni84 – 863Substrate bindingBy similarity
Regioni116 – 1172Substrate bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the asparaginase 1 family.Curated
Contains 5 ANK repeats.PROSITE-ProRule annotation
Contains 1 asparaginase/glutaminase domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiCOG0252.
GeneTreeiENSGT00390000001610.
HOGENOMiHOG000227974.
HOVERGENiHBG079531.
InParanoidiA0JNU3.
KOiK13278.
OMAiTICMRKS.
OrthoDBiEOG7WDN22.
PhylomeDBiA0JNU3.
TreeFamiTF315247.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR027475. Asparaginase/glutaminase_AS2.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFiPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR00139. ASNGLNASE.
SMARTiSM00248. ANK. 4 hits.
SM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00519. asnASE_I. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JNU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARAMGPERR LLAIYTGGTI GMRSEGGVLV PGRGLAAVLK TLHMFHDEEY
60 70 80 90 100
AQAHSLPEDT LVLPPASPDQ RIIYTVLECQ PLFDSSDMTI TEWVQIAQTI
110 120 130 140 150
ERHYAQYQGF VVIHGTDTMA FAASVLSFML ENLQKPVVLT GAQVPIHALW
160 170 180 190 200
SDGRENLLGA LLMAGQYVIP EVCLFFQNQL FRGNRTTKVD ARRFAAFCSP
210 220 230 240 250
NLPPLATVGA DVTINRELVR KACGKSHLVV HSSMEPDVGL LRLYPGIPAS
260 270 280 290 300
LVRTFLQPPL KGVVMETFGS GNGPTKPDLL QELRVAAEQG LIIVNCTHCL
310 320 330 340 350
QGAVTSDYAS GMAMAGAGIV SGFDMTSEAA LAKLSYVLGQ PGLSLNDRKK
360 370 380 390 400
LLAKDLRGEM TLPATDVLLQ DGMLGCRVAW LLSMNGSQEA DTMKDVLLPG
410 420 430 440 450
LALAAAHAGD LDTLQAFVEL DRDLNLKDYS GQTPLHVAAR RGHAAVVTML
460 470 480 490 500
LQRGADVDAR NEDGQSPLLL AVRGRHQSVI GLLRAAGARL SPQELEDVGT
510 520 530 540 550
ELCRLASRGD SEGLRAWWQA GADLGQPDYD GHCALQVAEA AGNADVVALL
560
QSFKDSVCAQ PQPH
Length:564
Mass (Da):60,595
Last modified:December 12, 2006 - v1
Checksum:i986677FD4963A55C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126962 mRNA. Translation: AAI26963.1.
CCDSiCCDS36567.1.
RefSeqiNP_001074638.1. NM_001081169.1.
UniGeneiMm.77133.

Genome annotation databases

EnsembliENSMUST00000079400; ENSMUSP00000078369; ENSMUSG00000037686.
GeneIDi104816.
KEGGimmu:104816.
UCSCiuc007pek.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126962 mRNA. Translation: AAI26963.1.
CCDSiCCDS36567.1.
RefSeqiNP_001074638.1. NM_001081169.1.
UniGeneiMm.77133.

3D structure databases

ProteinModelPortaliA0JNU3.
SMRiA0JNU3. Positions 8-550.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078369.

PTM databases

PhosphoSiteiA0JNU3.

Proteomic databases

PaxDbiA0JNU3.
PRIDEiA0JNU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079400; ENSMUSP00000078369; ENSMUSG00000037686.
GeneIDi104816.
KEGGimmu:104816.
UCSCiuc007pek.1. mouse.

Organism-specific databases

CTDi374569.
MGIiMGI:2144822. Aspg.

Phylogenomic databases

eggNOGiCOG0252.
GeneTreeiENSGT00390000001610.
HOGENOMiHOG000227974.
HOVERGENiHBG079531.
InParanoidiA0JNU3.
KOiK13278.
OMAiTICMRKS.
OrthoDBiEOG7WDN22.
PhylomeDBiA0JNU3.
TreeFamiTF315247.

Enzyme and pathway databases

BRENDAi3.1.1.47. 3474.

Miscellaneous databases

ChiTaRSiAspg. mouse.
NextBioi357306.
PROiA0JNU3.
SOURCEiSearch...

Gene expression databases

BgeeiA0JNU3.
CleanExiMM_A530050D06RIK.
GenevisibleiA0JNU3. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR027475. Asparaginase/glutaminase_AS2.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFiPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR00139. ASNGLNASE.
SMARTiSM00248. ANK. 4 hits.
SM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00519. asnASE_I. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiLPP60_MOUSE
AccessioniPrimary (citable) accession number: A0JNU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: December 12, 2006
Last modified: June 24, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.