Reviewed, UniProtKB/Swiss-Prot A0JNU3 (LPP60_MOUSE)

Names and origin

Protein names

Recommended name:
    60 kDa lysophospholipase
    EC=3.1.1.5
Including the following 2 domains:
    1- Recommended name:
            L-asparaginase
              EC=3.5.1.1
        Alternative name(s):
            L-asparagine amidohydrolase
    2- Recommended name:
            Platelet-activating factor acetylhydrolase
                Short name=PAF acetylhydrolase
              EC=3.1.1.47

Gene names

Name:

Aspg

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.
Catalytic activity	2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate. L-asparagine + H2O = L-aspartate + NH3. 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Subunit structure	Monomer By similarity.
Sequence similarities	In the N-terminal section; belongs to the asparaginase 1 family. Contains 5 ANK repeats.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	ANK repeat Repeat
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-alkyl-2-acetylglycerophosphocholine esterase activity Inferred from electronic annotation. Source: EC asparaginase activity Inferred from electronic annotation. Source: EC lysophospholipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	60 kDa lysophospholipase		PRO_0000324164
Regions
	Repeat	141 – 170	30	ANK 1
	Repeat	396 – 426	31	ANK 2
	Repeat	430 – 459	30	ANK 3
	Repeat	463 – 492	30	ANK 4
	Repeat	530 – 559	30	ANK 5
	Region	41 – 350	310	Asparaginase
Sites
	Active site	19	1	By similarity
	Active site	116	1	By similarity
	Active site	117	1	By similarity
	Active site	187	1	By similarity
Amino acid modifications
	Modified residue	387	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	A0JNU3-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 986677FD4963A55C	FASTA	564	60,595
	10 20 30 40 50 60 MARAMGPERR LLAIYTGGTI GMRSEGGVLV PGRGLAAVLK TLHMFHDEEY AQAHSLPEDT 70 80 90 100 110 120 LVLPPASPDQ RIIYTVLECQ PLFDSSDMTI TEWVQIAQTI ERHYAQYQGF VVIHGTDTMA 130 140 150 160 170 180 FAASVLSFML ENLQKPVVLT GAQVPIHALW SDGRENLLGA LLMAGQYVIP EVCLFFQNQL 190 200 210 220 230 240 FRGNRTTKVD ARRFAAFCSP NLPPLATVGA DVTINRELVR KACGKSHLVV HSSMEPDVGL 250 260 270 280 290 300 LRLYPGIPAS LVRTFLQPPL KGVVMETFGS GNGPTKPDLL QELRVAAEQG LIIVNCTHCL 310 320 330 340 350 360 QGAVTSDYAS GMAMAGAGIV SGFDMTSEAA LAKLSYVLGQ PGLSLNDRKK LLAKDLRGEM 370 380 390 400 410 420 TLPATDVLLQ DGMLGCRVAW LLSMNGSQEA DTMKDVLLPG LALAAAHAGD LDTLQAFVEL 430 440 450 460 470 480 DRDLNLKDYS GQTPLHVAAR RGHAAVVTML LQRGADVDAR NEDGQSPLLL AVRGRHQSVI 490 500 510 520 530 540 GLLRAAGARL SPQELEDVGT ELCRLASRGD SEGLRAWWQA GADLGQPDYD GHCALQVAEA 550 560 AGNADVVALL QSFKDSVCAQ PQPH

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC126962 mRNA. Translation: AAI26963.1.
IPI	IPI00341423.
RefSeq	NP_001074638.1.
UniGene	Mm.77133
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	A0JNU3.
Genome annotation databases
Ensembl	ENSMUSG00000037686. Mus musculus. [Contig view]
GeneID	104816.
KEGG	mmu:104816.
NMPDR	fig|10090.3.peg.27151.
Organism-specific databases
MGI	MGI:2144822. A530050D06Rik.
Phylogenomic databases
HOVERGEN	A0JNU3.
OMA	A0JNU3. FVIPEVC.
Enzyme and pathway databases
BRENDA	3.1.1.47. 244. 3.1.1.5. 244. 3.5.1.1. 244.
Gene expression databases
Bgee	A0JNU3.
CleanEx	MM_A530050D06RIK.
Family and domain databases
InterPro	IPR002110. ANK. IPR006033. AsnASEI. IPR006034. Asp/Glutamnse. [Graphical view]
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00023. Ank. 4 hits. PF00710. Asparaginase. 1 hit. [Graphical view]
PRINTS	PR00139. ASNGLNASE.
ProDom	PD003221. Asp/Glutamnse. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00248. ANK. 4 hits. [Graphical view]
TIGRFAMs	TIGR00519. asnASE_I. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. PS00144. ASN_GLN_ASE_1. False negative. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	357306.
SOURCE	Search...

Entry information

Entry name

LPP60_MOUSE

Accession

Primary (citable) accession number: A0JNU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)