A0JNK3 (HTRA2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine protease HTRA2, mitochondrial
EC=3.4.21.108

Gene names

Name:

HTRA2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis By similarity.
Catalytic activity	Cleavage of non-polar aliphatic amino-acids at the P1 position, with a preference for Val, Ile and Met. At the P2 and P3 positions, Arg is selected most strongly with a secondary preference for other hydrophilic residues.
Subunit structure	Homotrimer. Interacts with MXI2. Interacts with THAP5 under apoptotic conditions By similarity. The mature protein, but not the precursor, binds to BIRC2, BIRC3 and XIAP By similarity.
Subcellular location	Mitochondrion intermembrane space. Mitochondrion membrane; Single-pass membrane protein Potential. Note: Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID By similarity.
Domain	The mature N-terminus is involved in the interaction with XIAP By similarity. The PDZ domain mediates interaction with MXI2 By similarity.
Post-translational modification	Autoproteolytically activated By similarity.
Sequence similarities	Belongs to the peptidase S1B family. Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 31	31	Mitochondrion By similarity
	Propeptide	32 – 133	102	By similarity		PRO_0000287725
	Chain	134 – 458	325	Serine protease HTRA2, mitochondrial		PRO_0000287726
Regions
	Transmembrane	105 – 125	21	Helical; Potential
	Domain	364 – 445	82	PDZ
	Region	166 – 342	177	Serine protease
	Motif	134 – 137	4	IAP-binding motif
Sites
	Active site	198	1	Charge relay system By similarity
	Active site	228	1	Charge relay system By similarity
	Active site	306	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JNK3 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 9BFC45475D18D334	FASTA	458	49,163
	10 20 30 40 50 60 MAALRAGRGA GWSLRGWRAL WGGRWGKGPL LTPDLRALLT SGTPDPRTRV TYGTPSFRAR 70 80 90 100 110 120 LSVGVPEPRT CLRSRTSDLR ARLIAGTPDP RTPEDSGTPG TRLRVWLAVA LGAGGAVLLL 130 140 150 160 170 180 FWGGGRGPPA VLASVLGSPP TSPRSQYNFI ADVVEKTAPA VVYIEILGRH PFSGREVPIS 190 200 210 220 230 240 NGSGFVVAAD GLIVTNAHVV ADRRRVRVRL PSGDTYEAVV TAVDPVADIA TLRIQTKEPL 250 260 270 280 290 300 PTLPLGRSAD VRQGEFVVAM GSPFALQNTI TSGIVSSAQR PAKDLGLPQT NVEYIQTDAA 310 320 330 340 350 360 IDFGNSGGPL VNLDGEVIGV NTMKVTSGIS FAIPSDRLRE FLHRGEKKNS WFGISGSQRR 370 380 390 400 410 420 YIGVMMLTLT PSILAELQLR EPSFPDVQHG VLIHKVILDS PAHRAGLRPG DVILAIGEQL 430 440 450 VQNAEDIYEA VRTQSQLAVR IRRGQETLTL YVTPEVTE

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC126737 mRNA. Translation: AAI26738.1.
IPI	IPI00686966.
RefSeq	NP_001071424.1. NM_001077956.1.
UniGene	Bt.61801.
3D structure databases
ProteinModelPortal	A0JNK3.
SMR	A0JNK3. Positions 139-458.
ModBase	Search...
Protein-protein interaction databases
STRING	A0JNK3.
Proteomic databases
PRIDE	A0JNK3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000026804; ENSBTAP00000026804; ENSBTAG00000020124.
GeneID	523039.
KEGG	bta:523039.
Organism-specific databases
CTD	27429.
Phylogenomic databases
eggNOG	maNOG15484.
GeneTree	ENSGT00510000046315.
HOVERGEN	HBG052044.
InParanoid	A0JNK3.
OMA	VLVWKVI.
OrthoDB	EOG4J9N00.
PhylomeDB	A0JNK3.
Family and domain databases
InterPro	IPR001478. PDZ/DHR/GLGF. IPR009003. Pept_cys/ser_Trypsin-like. IPR001254. Peptidase_S1_S6. IPR001940. Peptidase_S1C. [Graphical view]
KO	K08669.
Pfam	PF00595. PDZ. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00834. PROTEASES2C.
SMART	SM00228. PDZ. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 1 hit. SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50106. PDZ. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HTRA2_BOVIN

Accession

Primary (citable) accession number: A0JNK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 12, 2006
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program