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Protein

Serine protease HTRA2, mitochondrial

Gene

HTRA2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis (By similarity).By similarity

Catalytic activityi

Cleavage of non-polar aliphatic amino-acids at the P1 position, with a preference for Val, Ile and Met. At the P2 and P3 positions, Arg is selected most strongly with a secondary preference for other hydrophilic residues.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei198 – 1981Charge relay systemBy similarity
Active sitei228 – 2281Charge relay systemBy similarity
Active sitei306 – 3061Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Apoptosis

Protein family/group databases

MEROPSiS01.278.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease HTRA2, mitochondrial (EC:3.4.21.108)
Gene namesi
Name:HTRA2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei105 – 12521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3131MitochondrionBy similarityAdd
BLAST
Propeptidei32 – 133102By similarityPRO_0000287725Add
BLAST
Chaini134 – 458325Serine protease HTRA2, mitochondrialPRO_0000287726Add
BLAST

Post-translational modificationi

Autoproteolytically activated.By similarity

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiA0JNK3.
PRIDEiA0JNK3.

Interactioni

Subunit structurei

Homotrimer. Interacts with MXI2. Interacts with THAP5 under apoptotic conditions (By similarity). The mature protein, but not the precursor, binds to BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4 (By similarity). Interacts with BIRC6/bruce (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026804.

Structurei

3D structure databases

ProteinModelPortaliA0JNK3.
SMRiA0JNK3. Positions 139-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini364 – 44582PDZAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 342177Serine proteaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi134 – 1374IAP-binding motif

Domaini

The mature N-terminus is involved in the interaction with XIAP.By similarity
The PDZ domain mediates interaction with MXI2.By similarity

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated
Contains 1 PDZ (DHR) domain.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
GeneTreeiENSGT00510000046315.
HOGENOMiHOG000223641.
HOVERGENiHBG052044.
InParanoidiA0JNK3.
KOiK08669.
OMAiFKRCIAS.
OrthoDBiEOG7V1FR7.
TreeFamiTF323480.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0JNK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALRAGRGA GWSLRGWRAL WGGRWGKGPL LTPDLRALLT SGTPDPRTRV
60 70 80 90 100
TYGTPSFRAR LSVGVPEPRT CLRSRTSDLR ARLIAGTPDP RTPEDSGTPG
110 120 130 140 150
TRLRVWLAVA LGAGGAVLLL FWGGGRGPPA VLASVLGSPP TSPRSQYNFI
160 170 180 190 200
ADVVEKTAPA VVYIEILGRH PFSGREVPIS NGSGFVVAAD GLIVTNAHVV
210 220 230 240 250
ADRRRVRVRL PSGDTYEAVV TAVDPVADIA TLRIQTKEPL PTLPLGRSAD
260 270 280 290 300
VRQGEFVVAM GSPFALQNTI TSGIVSSAQR PAKDLGLPQT NVEYIQTDAA
310 320 330 340 350
IDFGNSGGPL VNLDGEVIGV NTMKVTSGIS FAIPSDRLRE FLHRGEKKNS
360 370 380 390 400
WFGISGSQRR YIGVMMLTLT PSILAELQLR EPSFPDVQHG VLIHKVILDS
410 420 430 440 450
PAHRAGLRPG DVILAIGEQL VQNAEDIYEA VRTQSQLAVR IRRGQETLTL

YVTPEVTE
Length:458
Mass (Da):49,163
Last modified:December 12, 2006 - v1
Checksum:i9BFC45475D18D334
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126737 mRNA. Translation: AAI26738.1.
RefSeqiNP_001071424.1. NM_001077956.1.
UniGeneiBt.61801.

Genome annotation databases

EnsembliENSBTAT00000026804; ENSBTAP00000026804; ENSBTAG00000020124.
GeneIDi523039.
KEGGibta:523039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126737 mRNA. Translation: AAI26738.1.
RefSeqiNP_001071424.1. NM_001077956.1.
UniGeneiBt.61801.

3D structure databases

ProteinModelPortaliA0JNK3.
SMRiA0JNK3. Positions 139-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026804.

Protein family/group databases

MEROPSiS01.278.

Proteomic databases

PaxDbiA0JNK3.
PRIDEiA0JNK3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026804; ENSBTAP00000026804; ENSBTAG00000020124.
GeneIDi523039.
KEGGibta:523039.

Organism-specific databases

CTDi27429.

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
GeneTreeiENSGT00510000046315.
HOGENOMiHOG000223641.
HOVERGENiHBG052044.
InParanoidiA0JNK3.
KOiK08669.
OMAiFKRCIAS.
OrthoDBiEOG7V1FR7.
TreeFamiTF323480.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.

Entry informationi

Entry nameiHTRA2_BOVIN
AccessioniPrimary (citable) accession number: A0JNK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 12, 2006
Last modified: June 8, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.