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Protein

Serine protease HTRA2, mitochondrial

Gene

HTRA2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis (By similarity).By similarity

Catalytic activityi

Cleavage of non-polar aliphatic amino-acids at the P1 position, with a preference for Val, Ile and Met. At the P2 and P3 positions, Arg is selected most strongly with a secondary preference for other hydrophilic residues.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei198Charge relay systemBy similarity1
Active sitei228Charge relay systemBy similarity1
Active sitei306Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processApoptosis

Protein family/group databases

MEROPSiS01.278

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease HTRA2, mitochondrial (EC:3.4.21.108)
Gene namesi
Name:HTRA2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Organism-specific databases

VGNCiVGNC:30005 HTRA2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 31MitochondrionBy similarityAdd BLAST31
PropeptideiPRO_000028772532 – 133By similarityAdd BLAST102
ChainiPRO_0000287726134 – 458Serine protease HTRA2, mitochondrialAdd BLAST325

Post-translational modificationi

Autoproteolytically activated.By similarity

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiA0JNK3
PRIDEiA0JNK3

Expressioni

Gene expression databases

BgeeiENSBTAG00000020124

Interactioni

Subunit structurei

Homotrimer. Interacts with MXI2. Interacts with THAP5 under apoptotic conditions (By similarity). The mature protein, but not the precursor, binds to BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4 (By similarity). Interacts with BIRC6/bruce (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026804

Structurei

3D structure databases

ProteinModelPortaliA0JNK3
SMRiA0JNK3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini364 – 445PDZAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 342Serine proteaseAdd BLAST177

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi134 – 137IAP-binding motif4

Domaini

The mature N-terminus is involved in the interaction with XIAP.By similarity
The PDZ domain mediates interaction with MXI2.By similarity

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1320 Eukaryota
COG0265 LUCA
GeneTreeiENSGT00510000046315
HOGENOMiHOG000223641
HOVERGENiHBG052044
InParanoidiA0JNK3
KOiK08669
OMAiAHVVINK
OrthoDBiEOG091G0LXR
TreeFamiTF323480

Family and domain databases

InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
IPR009003 Peptidase_S1_PA
IPR001940 Peptidase_S1C
PfamiView protein in Pfam
PF13180 PDZ_2, 1 hit
PRINTSiPR00834 PROTEASES2C
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
SSF50494 SSF50494, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0JNK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALRAGRGA GWSLRGWRAL WGGRWGKGPL LTPDLRALLT SGTPDPRTRV
60 70 80 90 100
TYGTPSFRAR LSVGVPEPRT CLRSRTSDLR ARLIAGTPDP RTPEDSGTPG
110 120 130 140 150
TRLRVWLAVA LGAGGAVLLL FWGGGRGPPA VLASVLGSPP TSPRSQYNFI
160 170 180 190 200
ADVVEKTAPA VVYIEILGRH PFSGREVPIS NGSGFVVAAD GLIVTNAHVV
210 220 230 240 250
ADRRRVRVRL PSGDTYEAVV TAVDPVADIA TLRIQTKEPL PTLPLGRSAD
260 270 280 290 300
VRQGEFVVAM GSPFALQNTI TSGIVSSAQR PAKDLGLPQT NVEYIQTDAA
310 320 330 340 350
IDFGNSGGPL VNLDGEVIGV NTMKVTSGIS FAIPSDRLRE FLHRGEKKNS
360 370 380 390 400
WFGISGSQRR YIGVMMLTLT PSILAELQLR EPSFPDVQHG VLIHKVILDS
410 420 430 440 450
PAHRAGLRPG DVILAIGEQL VQNAEDIYEA VRTQSQLAVR IRRGQETLTL

YVTPEVTE
Length:458
Mass (Da):49,163
Last modified:December 12, 2006 - v1
Checksum:i9BFC45475D18D334
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126737 mRNA Translation: AAI26738.1
RefSeqiNP_001071424.1, NM_001077956.1
UniGeneiBt.61801

Genome annotation databases

EnsembliENSBTAT00000026804; ENSBTAP00000026804; ENSBTAG00000020124
GeneIDi523039
KEGGibta:523039

Similar proteinsi

Entry informationi

Entry nameiHTRA2_BOVIN
AccessioniPrimary (citable) accession number: A0JNK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 12, 2006
Last modified: March 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health