Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A0JNH9

- APLF_BOVIN

UniProt

A0JNH9 - APLF_BOVIN

Protein

Aprataxin and PNK-like factor

Gene

APLF

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (12 Dec 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage By similarity.By similarity

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei350 – 3501Poly-ADP-riboseBy similarity
    Binding sitei355 – 3551Poly-ADP-riboseBy similarity
    Binding sitei360 – 3601Poly-ADP-riboseBy similarity
    Binding sitei361 – 3611Poly-ADP-riboseBy similarity
    Binding sitei397 – 3971Poly-ADP-riboseBy similarity
    Binding sitei402 – 4021Poly-ADP-riboseBy similarity
    Binding sitei403 – 4031Poly-ADP-riboseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri351 – 37222PBZ-type 1Add
    BLAST
    Zinc fingeri393 – 41422PBZ-type 2Add
    BLAST

    GO - Molecular functioni

    1. 3'-5' exonuclease activity Source: UniProtKB
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB
    3. endodeoxyribonuclease activity Source: UniProtKB
    4. metal ion binding Source: UniProtKB-KW
    5. nucleotide binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: UniProtKB
    2. DNA catabolic process, endonucleolytic Source: GOC
    3. double-strand break repair Source: UniProtKB
    4. nucleic acid phosphodiester bond hydrolysis Source: GOC
    5. single strand break repair Source: UniProtKB

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aprataxin and PNK-like factor (EC:4.2.99.18)
    Alternative name(s):
    Apurinic-apyrimidinic endonuclease APLF
    Gene namesi
    Name:APLF
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 11

    Subcellular locationi

    Nucleus. Cytoplasmcytosol
    Note: Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 485485Aprataxin and PNK-like factorPRO_0000287355Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei92 – 921Phosphoserine; by ATMBy similarity

    Post-translational modificationi

    Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc fingers, the protein is also covalently poly-ADP-ribosylated by PARP1 By similarity.By similarity
    Phosphorylated in an ATM-dependent manner upon double-strand DNA break.By similarity

    Keywords - PTMi

    ADP-ribosylation, Phosphoprotein

    Proteomic databases

    PRIDEiA0JNH9.

    Interactioni

    Subunit structurei

    Interacts with LIG4, PARP1, XRCC1, XRCC4 and XRCC5.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000042099.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 8484FHA-likeAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni380 – 39011Flexible linkerBy similarityAdd
    BLAST

    Domaini

    The PBZ-type zinc fingers (also named CYR) mediate non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and promotes its recruitment to DNA damage sites By similarity.By similarity
    The FHA-like domain mediates interaction with XRCC1 and XRCC4.By similarity

    Sequence similaritiesi

    Belongs to the APLF family.Curated
    Contains 1 FHA-like domain.Curated
    Contains 2 PBZ-type zinc fingers.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri351 – 37222PBZ-type 1Add
    BLAST
    Zinc fingeri393 – 41422PBZ-type 2Add
    BLAST

    Keywords - Domaini

    Coiled coil, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG85452.
    GeneTreeiENSGT00390000010591.
    HOGENOMiHOG000033995.
    HOVERGENiHBG095728.
    InParanoidiA0JNH9.
    KOiK13295.

    Family and domain databases

    InterProiIPR008984. SMAD_FHA_domain.
    IPR019406. Znf_C2H2_APLF-like.
    [Graphical view]
    PfamiPF10283. zf-CCHH. 2 hits.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0JNH9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGGFELQPQ DGGPRVALAP GETVIGRGPL LGLHRNPCYY QSSEKSQLLP    50
    LKTNIWCWLN PGDHFSLLVD KYIFCVLSTH SEMEMECTLR NSQMLDEDDI 100
    LNEIPKSSSA DLPDKTPSAP RRERSTETAK PQAAANNMSF IGESRDLSKQ 150
    QPNPSERKRI LPAWMLTENS SDQNLSVISG GNNVTWESEK ERVCKDKTQV 200
    NITQPGKKRL ISSGSSESTS AKQDTGKKCK NDDQEESIIS SKEMPQSFSA 250
    AMLHNTEIDN TKTNPQRSKV PVEALGKVSE HKIITKGSSN EDSTARSCSE 300
    SYSSTQSKSF CDKPQKSHPE PSSNPPSPEC VQAKATDSVP NGSEENKVQR 350
    TSCMYGANCY RKNPVHFQHF SHPGDSDYGG VNITCQDEAD DRPECPYGAS 400
    CYRKNPQHKI EYRHSTFPVR SISDEDDNVG QPNEYNLNDS FIDDEEEEYE 450
    PTDEDSDWEP EKEDLEKEDM EGLLKEAKKF MKRKK 485
    Length:485
    Mass (Da):54,326
    Last modified:December 12, 2006 - v1
    Checksum:iD88D75D4D7C9D456
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC126691 mRNA. Translation: AAI26692.1.
    RefSeqiNP_001071342.1. NM_001077874.1.
    UniGeneiBt.63859.

    Genome annotation databases

    EnsembliENSBTAT00000044615; ENSBTAP00000042099; ENSBTAG00000018401.
    GeneIDi507989.
    KEGGibta:507989.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC126691 mRNA. Translation: AAI26692.1 .
    RefSeqi NP_001071342.1. NM_001077874.1.
    UniGenei Bt.63859.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000042099.

    Proteomic databases

    PRIDEi A0JNH9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000044615 ; ENSBTAP00000042099 ; ENSBTAG00000018401 .
    GeneIDi 507989.
    KEGGi bta:507989.

    Organism-specific databases

    CTDi 200558.

    Phylogenomic databases

    eggNOGi NOG85452.
    GeneTreei ENSGT00390000010591.
    HOGENOMi HOG000033995.
    HOVERGENi HBG095728.
    InParanoidi A0JNH9.
    KOi K13295.

    Miscellaneous databases

    NextBioi 20868301.

    Family and domain databases

    InterProi IPR008984. SMAD_FHA_domain.
    IPR019406. Znf_C2H2_APLF-like.
    [Graphical view ]
    Pfami PF10283. zf-CCHH. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Hypothalamus.

    Entry informationi

    Entry nameiAPLF_BOVIN
    AccessioniPrimary (citable) accession number: A0JNH9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3