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A0JNE3 (NR2C1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nuclear receptor subfamily 2 group C member 1
Gene names
Name:NR2C1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes including ESR1 and RARB. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences By similarity. Also activator of OCT4 gene expression. Plays a fundamental role in early embryogenesis and regulates embryonic stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation By similarity.

Subunit structure

Homodimer By similarity. Heterodimer; with NR2C2 which is required for chromatin remodeling and for binding to promoter regions such as globin DR1 repeats By similarity. Interacts with ESR1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with NRIP1 (via its LXXLL motifs); the interaction provides corepressor activity. Interacts with HDAC3 (via the DNA-binding domain); the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC4 (via the DNA-binding domain). Interacts with PIAS1; the interaction is required for sumoylation of NR2C1. Interacts with UBE2I; the interaction is required for sumoylation of NR2C1. Interacts with KAT2B; the interaction acts as a corepressor of gene expression By similarity.

Subcellular location

Nucleus By similarity. NucleusPML body By similarity. Note: Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-223 phosphorylation, to PML bodies for subsequent sumoylation By similarity.

Post-translational modification

Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression By similarity.

Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-223, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-586 by PKC is important for protein stability and function as activator of RARB By similarity.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR2 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 608608Nuclear receptor subfamily 2 group C member 1
PRO_0000369402

Regions

DNA binding111 – 18676Nuclear receptor
Zinc finger114 – 13421NR C4-type
Zinc finger150 – 16920NR C4-type
Region1 – 179179Required for interaction with KAT2B By similarity
Region589 – 60820Required for interaction with NRIP1 By similarity

Amino acid modifications

Modified residue1981Phosphoserine By similarity
Modified residue2161Phosphoserine By similarity
Modified residue2211Phosphothreonine By similarity
Modified residue2231Phosphothreonine; by MAPK1 By similarity
Modified residue5861Phosphoserine; by PKC By similarity
Cross-link251Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

Sequence LengthMass (Da)Tools
A0JNE3 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 49C110AEB8B74BE2

FASTA60867,714
        10         20         30         40         50         60 
MASIEEIAHQ IIEQQMGEIV TEQPTGQKIQ IVTALDHNTQ GKQFILTNHD GSTPNKVILA 

        70         80         90        100        110        120 
RQDSTPGKVF FTTPDAAGVN QLFFTTPDLS TQQLQFLTDN SSSEQGPNKV FDLCVVCGDK 

       130        140        150        160        170        180 
ASGRHYGEVT CEGCKGFFKR SIRKNLVYSC RGSKDCIINK HHRNRCQYCR LQRCIAFGMK 

       190        200        210        220        230        240 
QDSVQCERKP IEVSREKSSN CAASTEKIYI RKDLRSPLAA TPTFVTDSET ARSAGLLDSG 

       250        260        270        280        290        300 
MFVNIHQSGI KTESTMLMTP DKAVSCQGDL STLASVVTSL ANLGKTKDPA PNSNEVSMIE 

       310        320        330        340        350        360 
SLSNGDTSYT SVCEFHQEMQ TNGDVSRAFD TLAKALNPGE STACQSSGEG MEGNVHLIAG 

       370        380        390        400        410        420 
DSSINYIEKE GPLLSDSHVA FRLTMPSPMP EYLNVHYIGE SASRLLFLSM HWALSIPSFQ 

       430        440        450        460        470        480 
ALGQENSISL VKAYWNELFT LGLAQCWQVM NVATILATFV NCLHSSLQQD KISAERRKLL 

       490        500        510        520        530        540 
MEHIFKLQEF CNSMVKLCID GYEYAYLKAI VLFSPDHPGL ENMEQIEKFQ EKAYVEFQDY 

       550        560        570        580        590        600 
ITKTYPDDTY RLSRLLLRLP ALRLMNATIT EELFFKGLIG NVRIDSVIPH ILKMEPADYN 


SQIIGHSI 

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References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC126643 mRNA. Translation: AAI26644.1.
RefSeqNP_001071372.1. NM_001077904.1.
UniGeneBt.28215.

3D structure databases

ProteinModelPortalA0JNE3.
SMRA0JNE3. Positions 113-189.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000023577.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID511407.
KEGGbta:511407.

Organism-specific databases

CTD7181.

Phylogenomic databases

eggNOGNOG297520.
HOGENOMHOG000013058.
InParanoidA0JNE3.
KOK08543.

Family and domain databases

Gene3D1.10.565.10. 3 hits.
3.30.50.10. 1 hit.
InterProIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 3 hits.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20869910.

Entry information

Entry nameNR2C1_BOVIN
AccessionPrimary (citable) accession number: A0JNE3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 12, 2006
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families