Nuclear receptor subfamily 2 group C member 1

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A0JNE3 (NR2C1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 56. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nuclear receptor subfamily 2 group C member 1

Gene names

Name:

NR2C1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes including ESR1 and RARB. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences By similarity. Also activator of OCT4 gene expression. Plays a fundamental role in early embryogenesis and regulates embryonic stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation By similarity.
Subunit structure	Homodimer By similarity. Heterodimer; with NR2C2 which is required for chromatin remodeling and for binding to promoter regions such as globin DR1 repeats By similarity. Interacts with ESR1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with NRIP1 (via its LXXLL motifs); the interaction provides corepressor activity. Interacts with HDAC3 (via the DNA-binding domain); the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC4 (via the DNA-binding domain). Interacts with PIAS1; the interaction is required for sumoylation of NR2C1. Interacts with UBE2I; the interaction is required for sumoylation of NR2C1. Interacts with KAT2B; the interaction acts as a corepressor of gene expression By similarity.
Subcellular location	Nucleus By similarity. Nucleus › PML body By similarity. Note: Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-223 phosphorylation, to PML bodies for subsequent sumoylation By similarity.
Post-translational modification	Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression By similarity. Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-223, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-586 by PKC is important for protein stability and function as activator of RARB By similarity.
Sequence similarities	Belongs to the nuclear hormone receptor family. NR2 subfamily. Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Receptor Repressor
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	PML body Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro steroid hormone receptor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 608

608

Nuclear receptor subfamily 2 group C member 1

PRO_0000369402

Regions

DNA binding

111 – 186

Nuclear receptor

Zinc finger

114 – 134

NR C4-type

Zinc finger

150 – 169

NR C4-type

Region

1 – 179

179

Required for interaction with KAT2B By similarity

Region

589 – 608

Required for interaction with NRIP1 By similarity

Amino acid modifications

Modified residue

198

Phosphoserine By similarity

Modified residue

216

Phosphoserine By similarity

Modified residue

221

Phosphothreonine By similarity

Modified residue

223

Phosphothreonine; by MAPK1 By similarity

Modified residue

586

Phosphoserine; by PKC By similarity

Cross-link

251

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A0JNE3 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 49C110AEB8B74BE2
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FASTA

608

67,714

        10         20         30         40         50         60 
MASIEEIAHQ IIEQQMGEIV TEQPTGQKIQ IVTALDHNTQ GKQFILTNHD GSTPNKVILA 

        70         80         90        100        110        120 
RQDSTPGKVF FTTPDAAGVN QLFFTTPDLS TQQLQFLTDN SSSEQGPNKV FDLCVVCGDK 

       130        140        150        160        170        180 
ASGRHYGEVT CEGCKGFFKR SIRKNLVYSC RGSKDCIINK HHRNRCQYCR LQRCIAFGMK 

       190        200        210        220        230        240 
QDSVQCERKP IEVSREKSSN CAASTEKIYI RKDLRSPLAA TPTFVTDSET ARSAGLLDSG 

       250        260        270        280        290        300 
MFVNIHQSGI KTESTMLMTP DKAVSCQGDL STLASVVTSL ANLGKTKDPA PNSNEVSMIE 

       310        320        330        340        350        360 
SLSNGDTSYT SVCEFHQEMQ TNGDVSRAFD TLAKALNPGE STACQSSGEG MEGNVHLIAG 

       370        380        390        400        410        420 
DSSINYIEKE GPLLSDSHVA FRLTMPSPMP EYLNVHYIGE SASRLLFLSM HWALSIPSFQ 

       430        440        450        460        470        480 
ALGQENSISL VKAYWNELFT LGLAQCWQVM NVATILATFV NCLHSSLQQD KISAERRKLL 

       490        500        510        520        530        540 
MEHIFKLQEF CNSMVKLCID GYEYAYLKAI VLFSPDHPGL ENMEQIEKFQ EKAYVEFQDY 

       550        560        570        580        590        600 
ITKTYPDDTY RLSRLLLRLP ALRLMNATIT EELFFKGLIG NVRIDSVIPH ILKMEPADYN 


SQIIGHSI

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References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC126643 mRNA. Translation: AAI26644.1.
IPI	IPI00685366.
RefSeq	NP_001071372.1. NM_001077904.1.
UniGene	Bt.28215.
3D structure databases
ProteinModelPortal	A0JNE3.
SMR	A0JNE3. Positions 113-189.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000023577.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	511407.
KEGG	bta:511407.
Organism-specific databases
CTD	7181.
Phylogenomic databases
eggNOG	NOG297520.
HOGENOM	HOG000013058.
InParanoid	A0JNE3.
KO	K08543.
OrthoDB	EOG476JZT.
Family and domain databases
Gene3D	1.10.565.10. 3 hits. 3.30.50.10. 1 hit.
InterPro	IPR008946. Nucl_hormone_rcpt_ligand-bd. IPR000536. Nucl_hrmn_rcpt_lig-bd_core. IPR001723. Str_hrmn_rcpt. IPR001628. Znf_hrmn_rcpt. IPR013088. Znf_NHR/GATA. [Graphical view]
Pfam	PF00104. Hormone_recep. 1 hit. PF00105. zf-C4. 1 hit. [Graphical view]
PRINTS	PR00398. STRDHORMONER. PR00047. STROIDFINGER.
SMART	SM00430. HOLI. 1 hit. SM00399. ZnF_C4. 1 hit. [Graphical view]
SUPFAM	SSF48508. Str_ncl_receptor. 1 hit.
PROSITE	PS00031. NUCLEAR_REC_DBD_1. 1 hit. PS51030. NUCLEAR_REC_DBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20869910.

Entry information

Entry name

NR2C1_BOVIN

Accession

Primary (citable) accession number: A0JNE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 12, 2006
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents