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A0JNE3

- NR2C1_BOVIN

UniProt

A0JNE3 - NR2C1_BOVIN

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Protein

Nuclear receptor subfamily 2 group C member 1

Gene

NR2C1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes including ESR1 and RARB. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences (By similarity). Also activator of OCT4 gene expression. Plays a fundamental role in early embryogenesis and regulates embryonic stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi111 – 18676Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri114 – 13421NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri150 – 16920NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. steroid hormone receptor activity Source: InterPro
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 2 group C member 1
Gene namesi
Name:NR2C1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Nucleus PROSITE-ProRule annotation. NucleusPML body By similarity
Note: Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-223 phosphorylation, to PML bodies for subsequent sumoylation.By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Nuclear receptor subfamily 2 group C member 1PRO_0000369402Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei198 – 1981PhosphoserineBy similarity
Modified residuei216 – 2161PhosphoserineBy similarity
Modified residuei221 – 2211PhosphothreonineBy similarity
Modified residuei223 – 2231Phosphothreonine; by MAPK1By similarity
Cross-linki251 – 251Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei586 – 5861Phosphoserine; by PKCBy similarity

Post-translational modificationi

Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression (By similarity).By similarity
Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-223, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-586 by PKC is important for protein stability and function as activator of RARB (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Homodimer (By similarity). Heterodimer; with NR2C2 which is required for chromatin remodeling and for binding to promoter regions such as globin DR1 repeats (By similarity). Interacts with ESR1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with NRIP1 (via its LXXLL motifs); the interaction provides corepressor activity. Interacts with HDAC3 (via the DNA-binding domain); the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC4 (via the DNA-binding domain). Interacts with PIAS1; the interaction is required for sumoylation of NR2C1. Interacts with UBE2I; the interaction is required for sumoylation of NR2C1. Interacts with KAT2B; the interaction acts as a corepressor of gene expression (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000023577.

Structurei

3D structure databases

ProteinModelPortaliA0JNE3.
SMRiA0JNE3. Positions 113-189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 179179Required for interaction with KAT2BBy similarityAdd
BLAST
Regioni589 – 60820Required for interaction with NRIP1By similarityAdd
BLAST

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri114 – 13421NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri150 – 16920NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG297520.
HOGENOMiHOG000013058.
InParanoidiA0JNE3.
KOiK08543.

Family and domain databases

Gene3Di1.10.565.10. 3 hits.
3.30.50.10. 1 hit.
InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 3 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JNE3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASIEEIAHQ IIEQQMGEIV TEQPTGQKIQ IVTALDHNTQ GKQFILTNHD
60 70 80 90 100
GSTPNKVILA RQDSTPGKVF FTTPDAAGVN QLFFTTPDLS TQQLQFLTDN
110 120 130 140 150
SSSEQGPNKV FDLCVVCGDK ASGRHYGEVT CEGCKGFFKR SIRKNLVYSC
160 170 180 190 200
RGSKDCIINK HHRNRCQYCR LQRCIAFGMK QDSVQCERKP IEVSREKSSN
210 220 230 240 250
CAASTEKIYI RKDLRSPLAA TPTFVTDSET ARSAGLLDSG MFVNIHQSGI
260 270 280 290 300
KTESTMLMTP DKAVSCQGDL STLASVVTSL ANLGKTKDPA PNSNEVSMIE
310 320 330 340 350
SLSNGDTSYT SVCEFHQEMQ TNGDVSRAFD TLAKALNPGE STACQSSGEG
360 370 380 390 400
MEGNVHLIAG DSSINYIEKE GPLLSDSHVA FRLTMPSPMP EYLNVHYIGE
410 420 430 440 450
SASRLLFLSM HWALSIPSFQ ALGQENSISL VKAYWNELFT LGLAQCWQVM
460 470 480 490 500
NVATILATFV NCLHSSLQQD KISAERRKLL MEHIFKLQEF CNSMVKLCID
510 520 530 540 550
GYEYAYLKAI VLFSPDHPGL ENMEQIEKFQ EKAYVEFQDY ITKTYPDDTY
560 570 580 590 600
RLSRLLLRLP ALRLMNATIT EELFFKGLIG NVRIDSVIPH ILKMEPADYN

SQIIGHSI
Length:608
Mass (Da):67,714
Last modified:December 12, 2006 - v1
Checksum:i49C110AEB8B74BE2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC126643 mRNA. Translation: AAI26644.1.
RefSeqiNP_001071372.1. NM_001077904.1.
UniGeneiBt.28215.

Genome annotation databases

GeneIDi511407.
KEGGibta:511407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC126643 mRNA. Translation: AAI26644.1 .
RefSeqi NP_001071372.1. NM_001077904.1.
UniGenei Bt.28215.

3D structure databases

ProteinModelPortali A0JNE3.
SMRi A0JNE3. Positions 113-189.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000023577.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 511407.
KEGGi bta:511407.

Organism-specific databases

CTDi 7181.

Phylogenomic databases

eggNOGi NOG297520.
HOGENOMi HOG000013058.
InParanoidi A0JNE3.
KOi K08543.

Miscellaneous databases

NextBioi 20869910.

Family and domain databases

Gene3Di 1.10.565.10. 3 hits.
3.30.50.10. 1 hit.
InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
Pfami PF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view ]
PRINTSi PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTi SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view ]
SUPFAMi SSF48508. SSF48508. 3 hits.
PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiNR2C1_BOVIN
AccessioniPrimary (citable) accession number: A0JNE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: December 12, 2006
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3