A0JNE3 (NR2C1_BOVIN) Reviewed, UniProtKB/Swiss-Prot
Last modified November 13, 2013. Version 59. History...
Names and origin
|Protein names||Recommended name:|
Nuclear receptor subfamily 2 group C member 1
|Organism||Bos taurus (Bovine) [Reference proteome]|
|Taxonomic identifier||9913 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos|
|Sequence length||608 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes including ESR1 and RARB. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences By similarity. Also activator of OCT4 gene expression. Plays a fundamental role in early embryogenesis and regulates embryonic stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation By similarity.
Homodimer By similarity. Heterodimer; with NR2C2 which is required for chromatin remodeling and for binding to promoter regions such as globin DR1 repeats By similarity. Interacts with ESR1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with NRIP1 (via its LXXLL motifs); the interaction provides corepressor activity. Interacts with HDAC3 (via the DNA-binding domain); the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC4 (via the DNA-binding domain). Interacts with PIAS1; the interaction is required for sumoylation of NR2C1. Interacts with UBE2I; the interaction is required for sumoylation of NR2C1. Interacts with KAT2B; the interaction acts as a corepressor of gene expression By similarity.
Nucleus By similarity. Nucleus › PML body By similarity. Note: Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-223 phosphorylation, to PML bodies for subsequent sumoylation By similarity.
Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression By similarity.
Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-223, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-586 by PKC is important for protein stability and function as activator of RARB By similarity.
Contains 1 nuclear receptor DNA-binding domain.
|Technical term||Complete proteome|
|Gene Ontology (GO)|
Inferred from electronic annotation. Source: UniProtKB-KW
Inferred from electronic annotation. Source: UniProtKB-SubCell
|Molecular_function||sequence-specific DNA binding|
Inferred from electronic annotation. Source: InterProsequence-specific DNA binding transcription factor activity
Inferred from electronic annotation. Source: InterProsteroid hormone receptor activity
Inferred from electronic annotation. Source: InterProzinc ion binding
Inferred from electronic annotation. Source: InterPro
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 608||608||Nuclear receptor subfamily 2 group C member 1||PRO_0000369402|
|DNA binding||111 – 186||76||Nuclear receptor|
|Zinc finger||114 – 134||21||NR C4-type|
|Zinc finger||150 – 169||20||NR C4-type|
|Region||1 – 179||179||Required for interaction with KAT2B By similarity|
|Region||589 – 608||20||Required for interaction with NRIP1 By similarity|
Amino acid modifications
|Modified residue||198||1||Phosphoserine By similarity|
|Modified residue||216||1||Phosphoserine By similarity|
|Modified residue||221||1||Phosphothreonine By similarity|
|Modified residue||223||1||Phosphothreonine; by MAPK1 By similarity|
|Modified residue||586||1||Phosphoserine; by PKC By similarity|
|Cross-link||251||Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity|
|BC126643 mRNA. Translation: AAI26644.1.|
|RefSeq||NP_001071372.1. NM_001077904.1. |
3D structure databases
|SMR||A0JNE3. Positions 113-189. |
Protein-protein interaction databases
Protocols and materials databases
Genome annotation databases
Enzyme and pathway databases
Family and domain databases
|Gene3D||1.10.565.10. 3 hits. |
184.108.40.206. 1 hit.
|InterPro||IPR008946. Nucl_hormone_rcpt_ligand-bd. |
|Pfam||PF00104. Hormone_recep. 1 hit. |
PF00105. zf-C4. 1 hit.
|PRINTS||PR00398. STRDHORMONER. |
|SMART||SM00430. HOLI. 1 hit. |
SM00399. ZnF_C4. 1 hit.
|SUPFAM||SSF48508. SSF48508. 3 hits. |
|PROSITE||PS00031. NUCLEAR_REC_DBD_1. 1 hit. |
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
|Accession||Primary (citable) accession number: A0JNE3|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families