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A0JNE3

- NR2C1_BOVIN

UniProt

A0JNE3 - NR2C1_BOVIN

Protein

Nuclear receptor subfamily 2 group C member 1

Gene

NR2C1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes including ESR1 and RARB. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences By similarity. Also activator of OCT4 gene expression. Plays a fundamental role in early embryogenesis and regulates embryonic stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi111 – 18676Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri114 – 13421NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri150 – 16920NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding transcription factor activity Source: InterPro
    3. steroid hormone receptor activity Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Receptor, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 2 group C member 1
    Gene namesi
    Name:NR2C1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation. NucleusPML body By similarity
    Note: Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-223 phosphorylation, to PML bodies for subsequent sumoylation.By similarity

    GO - Cellular componenti

    1. PML body Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 608608Nuclear receptor subfamily 2 group C member 1PRO_0000369402Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei198 – 1981PhosphoserineBy similarity
    Modified residuei216 – 2161PhosphoserineBy similarity
    Modified residuei221 – 2211PhosphothreonineBy similarity
    Modified residuei223 – 2231Phosphothreonine; by MAPK1By similarity
    Cross-linki251 – 251Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei586 – 5861Phosphoserine; by PKCBy similarity

    Post-translational modificationi

    Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression By similarity.By similarity
    Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-223, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-586 by PKC is important for protein stability and function as activator of RARB By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Interactioni

    Subunit structurei

    Homodimer By similarity. Heterodimer; with NR2C2 which is required for chromatin remodeling and for binding to promoter regions such as globin DR1 repeats By similarity. Interacts with ESR1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with NRIP1 (via its LXXLL motifs); the interaction provides corepressor activity. Interacts with HDAC3 (via the DNA-binding domain); the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC4 (via the DNA-binding domain). Interacts with PIAS1; the interaction is required for sumoylation of NR2C1. Interacts with UBE2I; the interaction is required for sumoylation of NR2C1. Interacts with KAT2B; the interaction acts as a corepressor of gene expression By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000023577.

    Structurei

    3D structure databases

    ProteinModelPortaliA0JNE3.
    SMRiA0JNE3. Positions 113-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 179179Required for interaction with KAT2BBy similarityAdd
    BLAST
    Regioni589 – 60820Required for interaction with NRIP1By similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri114 – 13421NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri150 – 16920NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG297520.
    HOGENOMiHOG000013058.
    InParanoidiA0JNE3.
    KOiK08543.

    Family and domain databases

    Gene3Di1.10.565.10. 3 hits.
    3.30.50.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 3 hits.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0JNE3-1 [UniParc]FASTAAdd to Basket

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    MASIEEIAHQ IIEQQMGEIV TEQPTGQKIQ IVTALDHNTQ GKQFILTNHD    50
    GSTPNKVILA RQDSTPGKVF FTTPDAAGVN QLFFTTPDLS TQQLQFLTDN 100
    SSSEQGPNKV FDLCVVCGDK ASGRHYGEVT CEGCKGFFKR SIRKNLVYSC 150
    RGSKDCIINK HHRNRCQYCR LQRCIAFGMK QDSVQCERKP IEVSREKSSN 200
    CAASTEKIYI RKDLRSPLAA TPTFVTDSET ARSAGLLDSG MFVNIHQSGI 250
    KTESTMLMTP DKAVSCQGDL STLASVVTSL ANLGKTKDPA PNSNEVSMIE 300
    SLSNGDTSYT SVCEFHQEMQ TNGDVSRAFD TLAKALNPGE STACQSSGEG 350
    MEGNVHLIAG DSSINYIEKE GPLLSDSHVA FRLTMPSPMP EYLNVHYIGE 400
    SASRLLFLSM HWALSIPSFQ ALGQENSISL VKAYWNELFT LGLAQCWQVM 450
    NVATILATFV NCLHSSLQQD KISAERRKLL MEHIFKLQEF CNSMVKLCID 500
    GYEYAYLKAI VLFSPDHPGL ENMEQIEKFQ EKAYVEFQDY ITKTYPDDTY 550
    RLSRLLLRLP ALRLMNATIT EELFFKGLIG NVRIDSVIPH ILKMEPADYN 600
    SQIIGHSI 608
    Length:608
    Mass (Da):67,714
    Last modified:December 12, 2006 - v1
    Checksum:i49C110AEB8B74BE2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC126643 mRNA. Translation: AAI26644.1.
    RefSeqiNP_001071372.1. NM_001077904.1.
    UniGeneiBt.28215.

    Genome annotation databases

    GeneIDi511407.
    KEGGibta:511407.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC126643 mRNA. Translation: AAI26644.1 .
    RefSeqi NP_001071372.1. NM_001077904.1.
    UniGenei Bt.28215.

    3D structure databases

    ProteinModelPortali A0JNE3.
    SMRi A0JNE3. Positions 113-189.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000023577.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 511407.
    KEGGi bta:511407.

    Organism-specific databases

    CTDi 7181.

    Phylogenomic databases

    eggNOGi NOG297520.
    HOGENOMi HOG000013058.
    InParanoidi A0JNE3.
    KOi K08543.

    Miscellaneous databases

    NextBioi 20869910.

    Family and domain databases

    Gene3Di 1.10.565.10. 3 hits.
    3.30.50.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 3 hits.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.

    Entry informationi

    Entry nameiNR2C1_BOVIN
    AccessioniPrimary (citable) accession number: A0JNE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3