Reviewed, UniProtKB/Swiss-Prot A0JND9 (ENTP8_BOVIN)

Names and origin

Protein names

Recommended name:
    Ectonucleoside triphosphate diphosphohydrolase 8
      Short name=E-NTPDase 8
      Short name=NTPDase 8
      Short name=NTPDase8
    EC=3.6.1.5

Gene names

Name:

ENTPD8

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolyzis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP By similarity.
Catalytic activity	ATP + 2 H2O = AMP + 2 phosphate.
Cofactor	Calcium By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The transmembranous domains are involved in regulation of enzyme activity By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Calcium Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Ectonucleoside triphosphate diphosphohydrolase 8		PRO_0000306881
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Potential
	Topological domain	30 – 466	437	Extracellular Potential
	Transmembrane	467 – 487	21	Potential
	Topological domain	488 – 495	8	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Glycosylation	324	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A0JND9-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 99082624BE7045F7	FASTA	495	53,240
	10 20 30 40 50 60 MGLTWKQRVF TALLGAAAVS GLTALLLVLV GTMNVLLPPD TKFGIVFDAG SSHTSLFVYQ 70 80 90 100 110 120 WPANKENDTG IVSQALACQA EGPGISSYAS NPARVGESLQ GCLEEALALI PKAKHHETPM 130 140 150 160 170 180 FLGATAGMRL LSRKNRSQAE DVFAAVSRAL GQSPVDFRGA ELLTGQDEGA FGWITINYIL 190 200 210 220 230 240 GLLVKYSFSG EWIRPLEETL VGALDMGGAS TQITFVPGGP ILDKTAQATF RLYGADHTVY 250 260 270 280 290 300 THSYLCFGRD QALSRVLAEL VQASTGLLIR HPCYHSGYRG TLALASLYES PCAPAAPPDL 310 320 330 340 350 360 SQNLTVEGTG NPGACVEALR KLFNFSSCDG REDCAFAGVY QPPVQGQFYA FSNFYYTFNF 370 380 390 400 410 420 LNLTSKPSLS GANATIWEFC LRPWKLVEAS APPGQDRWLR DYCASGLYIL TLLVEGYGFS 430 440 450 460 470 480 EETWGGIEFR QQAGGADIGW TLGYMLNLTN LIPAEAPAQG WAQSFGVWAA GVVFVVLTLA 490 ATLGAVAVQV FWLQD

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC126637 mRNA. Translation: AAI26638.1.
IPI	IPI00825666.
RefSeq	NP_001071395.1.
UniGene	Bt.42111
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0JND9.
Genome annotation databases
Ensembl	ENSBTAT00000016080; ENSBTAP00000016080; ENSBTAG00000030246; Bos taurus. [Genome view]
GeneID	515532.
KEGG	bta:515532.
Organism-specific databases
CTD	515532.
Phylogenomic databases
HOVERGEN	A0JND9.
Enzyme and pathway databases
BRENDA	3.6.1.5. 251.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. [Graphical view]
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENTP8_BOVIN

Accession

Primary (citable) accession number: A0JND9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	December 12, 2006
Last modified:	October 13, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)