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Protein

Ectonucleoside triphosphate diphosphohydrolase 8

Gene

ENTPD8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP (By similarity).By similarity

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Ca2+By similarity, Mg2+By similarityNote: Ca(2+) or Mg(2+). Has lower efficiency with Mg2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Proton acceptorBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 8 (EC:3.6.1.5)
Short name:
E-NTPDase 8
Short name:
NTPDase 8
Short name:
NTPDase8
Gene namesi
Name:ENTPD8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

  1. Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence Analysis
Transmembranei9 – 2921HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 466437ExtracellularSequence AnalysisAdd
BLAST
Transmembranei467 – 48721HelicalSequence AnalysisAdd
BLAST
Topological domaini488 – 4958CytoplasmicSequence Analysis

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Ectonucleoside triphosphate diphosphohydrolase 8PRO_0000306881Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi78 ↔ 102By similarity
Disulfide bondi246 ↔ 292By similarity
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi328 ↔ 334By similarity
Disulfide bondi380 ↔ 403By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiA0JND9.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000016080.

Family & Domainsi

Domaini

The transmembranous domains are involved in regulation of enzyme activity.By similarity

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiA0JND9.
KOiK01510.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JND9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTWKQRVF TALLGAAAVS GLTALLLVLV GTMNVLLPPD TKFGIVFDAG
60 70 80 90 100
SSHTSLFVYQ WPANKENDTG IVSQALACQA EGPGISSYAS NPARVGESLQ
110 120 130 140 150
GCLEEALALI PKAKHHETPM FLGATAGMRL LSRKNRSQAE DVFAAVSRAL
160 170 180 190 200
GQSPVDFRGA ELLTGQDEGA FGWITINYIL GLLVKYSFSG EWIRPLEETL
210 220 230 240 250
VGALDMGGAS TQITFVPGGP ILDKTAQATF RLYGADHTVY THSYLCFGRD
260 270 280 290 300
QALSRVLAEL VQASTGLLIR HPCYHSGYRG TLALASLYES PCAPAAPPDL
310 320 330 340 350
SQNLTVEGTG NPGACVEALR KLFNFSSCDG REDCAFAGVY QPPVQGQFYA
360 370 380 390 400
FSNFYYTFNF LNLTSKPSLS GANATIWEFC LRPWKLVEAS APPGQDRWLR
410 420 430 440 450
DYCASGLYIL TLLVEGYGFS EETWGGIEFR QQAGGADIGW TLGYMLNLTN
460 470 480 490
LIPAEAPAQG WAQSFGVWAA GVVFVVLTLA ATLGAVAVQV FWLQD
Length:495
Mass (Da):53,240
Last modified:December 12, 2006 - v1
Checksum:i99082624BE7045F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126637 mRNA. Translation: AAI26638.1.
RefSeqiNP_001071395.1. NM_001077927.2.
UniGeneiBt.42111.

Genome annotation databases

GeneIDi515532.
KEGGibta:515532.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126637 mRNA. Translation: AAI26638.1.
RefSeqiNP_001071395.1. NM_001077927.2.
UniGeneiBt.42111.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000016080.

Proteomic databases

PRIDEiA0JND9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi515532.
KEGGibta:515532.

Organism-specific databases

CTDi377841.

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiA0JND9.
KOiK01510.

Miscellaneous databases

NextBioi20871868.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiENTP8_BOVIN
AccessioniPrimary (citable) accession number: A0JND9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: December 12, 2006
Last modified: April 1, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.