A0JND3 (EOGT_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EGF domain-specific O-linked N-acetylglucosamine transferase
EC=2.4.1.255

Alternative name(s):
Extracellular O-linked N-acetylglucosamine transferase

Gene names

Name:	EOGT
Synonyms:	AER61

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine. UDP-N-acetyl-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-threonine.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the glycosyltransferase 61 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein O-linked glycosylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein N-acetylglucosaminyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 527	510	EGF domain-specific O-linked N-acetylglucosamine transferase		PRO_0000301969
Regions
	Motif	524 – 527	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	354	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A0JND3 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 1BDB2867FD8D13A1	FASTA	527	61,749
	10 20 30 40 50 60 MFMLLVFGAL LPEVPLSGQD KAPPQADGIS ATPLFNYASL RLPEEHIPFF LHNNRHIATV 70 80 90 100 110 120 CRKDSHCPYK KYLENLKYCW GYEKSCRPEF RFGYPVCTYV DMGWTDTLES AQEIFWKQAD 130 140 150 160 170 180 FGYAAERLEE LHVLCQPKEK NDSSLVCSRY LQYCRATNIY LDLRNIKRNH DRFKEDFVQS 190 200 210 220 230 240 GEIGGYCKLD IRSLMSQGQR KSPLQSWFAE LQSYTELNFR PVEDAQCDIV IEKPTYFMKL 250 260 270 280 290 300 DAGVNMYHHF CDFINLYITQ HVNNSFSTDV YVVMWDTSSY GYGDLFSDTW KAFTDYDVIH 310 320 330 340 350 360 LKTYDAKRVC FKEAIFSLLP RMRYGLFYNT PLISGCQNTG LFRAFSQHVL HRLNITQEGP 370 380 390 400 410 420 KGGKIRVTIL ARSTEYRKIL NQNELVNALK TVSTFEVQIV DYKYKELGFL DQLRITHNTD 430 440 450 460 470 480 IFIGMHGAGL THLLFLPDWA AVFELYNCGD ERCYLDLARL RGVHYITWRR QNKVFPQDKG 490 500 510 520 HHPTLGEHPK FTNYSFDVEE FMYLVLQAAD YVLQHPKWPF KKKHDEL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal skin.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC126625 mRNA. Translation: AAI26626.1.
IPI	IPI00694175.
RefSeq	NP_001071350.1. NM_001077882.1.
UniGene	Bt.21068.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT61. Glycosyltransferase Family 61.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000030754; ENSBTAP00000030725; ENSBTAG00000022681.
GeneID	508782.
KEGG	bta:508782.
Organism-specific databases
CTD	285203.
Phylogenomic databases
eggNOG	NOG320328.
GeneTree	ENSGT00390000008553.
HOGENOM	HOG000033829.
HOVERGEN	HBG056678.
InParanoid	A0JND3.
OMA	DSKRVCF.
OrthoDB	EOG4XGZZQ.
Enzyme and pathway databases
BioCyc	CATTLE:508782-MONOMER.
Family and domain databases
InterPro	IPR007657. Glycosyltransferase_AER61. [Graphical view]
Pfam	PF04577. DUF563. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20868679.

Entry information

Entry name

EOGT_BOVIN

Accession

Primary (citable) accession number: A0JND3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	December 12, 2006
Last modified:	May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program