Reviewed, UniProtKB/Swiss-Prot A0JN54 (DGKA_BOVIN)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase alpha
      Short name=DAG kinase alpha
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase alpha
      Short name=DGK-alpha

Gene names

Name:

DGKA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity By similarity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Enzyme regulation	Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 EF-hand domains. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 734	734	Diacylglycerol kinase alpha		PRO_0000281914
Regions
	Domain	109 – 144	36	EF-hand 1
	Domain	154 – 189	36	EF-hand 2
	Domain	371 – 505	135	DAGKc
	Calcium binding	122 – 133	12	1 Probable
	Calcium binding	167 – 178	12	2 Probable
	Zinc finger	204 – 252	49	Phorbol-ester/DAG-type 1
	Zinc finger	268 – 318	51	Phorbol-ester/DAG-type 2
Amino acid modifications
	Modified residue	22	1	Phosphotyrosine By similarity
	Modified residue	483	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JN54-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 63B02FAC44AB473A	FASTA	734	82,672
	10 20 30 40 50 60 MAKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA EYLQGDAIGY EGFQQFLKIY 70 80 90 100 110 120 LEVDNVPDHL SQALFQSFQT GYYIEDTVRE DVVCLSDVSC YFSLLEGGRP EDKLEFTFKL 130 140 150 160 170 180 YDTDRNGILD SSEVDRIIIQ MMRMAEYLDW DVSELRPILQ EMMKEIDYDG SGSVSLAEWL 190 200 210 220 230 240 RAGATTVPLL VLLGLEMTLK DNGQHMWRPK RFPRPVYCNL CESSIGLGKQ GLSCNLCKYI 250 260 270 280 290 300 VHDQCAMKAL PCEVSTYAKS RKDIGVQSHV WVRGGCESGR CDRCQKKIRI YHSLVGLHCV 310 320 330 340 350 360 WCHLEIHDDC LPAMGHECDC GLLRDHILPP SSIYPSVLAS GQERKTSKIS QKTMDDLSLS 370 380 390 400 410 420 TSEALRIDPV SNTHPLLVFV NPKSGGKQGE RVLWKFQYLL NPRQVFNLLK DGPEPGLRFF 430 440 450 460 470 480 RDVPDYRILV CGGDGTVGWI LESIDKANLP FVPPVAVLPL GTGNDLARCL RWGGGYEGQN 490 500 510 520 530 540 LGKILKDLET SKVVHMDRWS VEVIPQQTEE KSDPVPFQII NNYFSIGVDA SIAHRFHIMR 550 560 570 580 590 600 EKYPEKFNSR MKNKLWYFEF ATSESIFSTC KKLEESLTVE ICGKPLDLSN LSLEGIAVLN 610 620 630 640 650 660 IPSTHGGSNL WGDTKRPHGD IHGINQALGA TAKVITDPDI LKTCVPDLSD KRLEVVGLEG 670 680 690 700 710 720 AIEIGQIYTK LKNAGHRLAK CSEITFHTTK TLPMQIDGEP WMQTPCTIKI THRNQMPMLV 730 GPPPRSSNFF GFLC

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal skin.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC126521 mRNA. Translation: AAI26522.1.
IPI	IPI00701903.
RefSeq	NP_001071328.1.
UniGene	Bt.5091
3D structure databases
SMR	A0JN54. Positions 1-117.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000038186; ENSBTAP00000038002; ENSBTAG00000004018; Bos taurus. [Genome view]
GeneID	506348.
KEGG	bta:506348.
Organism-specific databases
CTD	506348.
Phylogenomic databases
HOVERGEN	A0JN54.
OMA	LWGDTKR.
Enzyme and pathway databases
BRENDA	2.7.1.107. 251.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kinase_access. IPR001206. Diacylglycerol_kinase_cat. IPR011992. EF-Hand_type. IPR018248. EF_hand. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00036. efhand. 1 hit. [Graphical view]
ProDom	PD002939. DAGKa. 1 hit. PD005043. DAGKc. 1 hit. PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGKA_BOVIN

Accession

Primary (citable) accession number: A0JN54

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)