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A0JN54

- DGKA_BOVIN

UniProt

A0JN54 - DGKA_BOVIN

Protein

Diacylglycerol kinase alpha

Gene

DGKA

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Enzyme regulationi

    Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi122 – 133121CuratedAdd
    BLAST
    Calcium bindingi167 – 178122CuratedAdd
    BLAST
    Zinc fingeri204 – 25249Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri268 – 31851Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. diacylglycerol kinase activity Source: UniProtKB-EC
    4. NAD+ kinase activity Source: InterPro
    5. phospholipid binding Source: Ensembl

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_216264. Effects of PIP2 hydrolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase alpha (EC:2.7.1.107)
    Short name:
    DAG kinase alpha
    Alternative name(s):
    Diglyceride kinase alpha
    Short name:
    DGK-alpha
    Gene namesi
    Name:DGKA
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. plasma membrane Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 734734Diacylglycerol kinase alphaPRO_0000281914Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei483 – 4831N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiA0JN54.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000038002.

    Structurei

    3D structure databases

    ProteinModelPortaliA0JN54.
    SMRiA0JN54. Positions 1-117.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini109 – 14436EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini154 – 18936EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini371 – 505135DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri204 – 25249Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri268 – 31851Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    GeneTreeiENSGT00750000117569.
    HOGENOMiHOG000252931.
    HOVERGENiHBG051345.
    InParanoidiA0JN54.
    KOiK00901.
    OMAiLVGLHCV.
    OrthoDBiEOG75XGK8.
    TreeFamiTF313104.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0JN54-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA EYLQGDAIGY    50
    EGFQQFLKIY LEVDNVPDHL SQALFQSFQT GYYIEDTVRE DVVCLSDVSC 100
    YFSLLEGGRP EDKLEFTFKL YDTDRNGILD SSEVDRIIIQ MMRMAEYLDW 150
    DVSELRPILQ EMMKEIDYDG SGSVSLAEWL RAGATTVPLL VLLGLEMTLK 200
    DNGQHMWRPK RFPRPVYCNL CESSIGLGKQ GLSCNLCKYI VHDQCAMKAL 250
    PCEVSTYAKS RKDIGVQSHV WVRGGCESGR CDRCQKKIRI YHSLVGLHCV 300
    WCHLEIHDDC LPAMGHECDC GLLRDHILPP SSIYPSVLAS GQERKTSKIS 350
    QKTMDDLSLS TSEALRIDPV SNTHPLLVFV NPKSGGKQGE RVLWKFQYLL 400
    NPRQVFNLLK DGPEPGLRFF RDVPDYRILV CGGDGTVGWI LESIDKANLP 450
    FVPPVAVLPL GTGNDLARCL RWGGGYEGQN LGKILKDLET SKVVHMDRWS 500
    VEVIPQQTEE KSDPVPFQII NNYFSIGVDA SIAHRFHIMR EKYPEKFNSR 550
    MKNKLWYFEF ATSESIFSTC KKLEESLTVE ICGKPLDLSN LSLEGIAVLN 600
    IPSTHGGSNL WGDTKRPHGD IHGINQALGA TAKVITDPDI LKTCVPDLSD 650
    KRLEVVGLEG AIEIGQIYTK LKNAGHRLAK CSEITFHTTK TLPMQIDGEP 700
    WMQTPCTIKI THRNQMPMLV GPPPRSSNFF GFLC 734
    Length:734
    Mass (Da):82,672
    Last modified:December 12, 2006 - v1
    Checksum:i63B02FAC44AB473A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC126521 mRNA. Translation: AAI26522.1.
    RefSeqiNP_001071328.1. NM_001077860.1.
    XP_005206588.1. XM_005206531.1.
    UniGeneiBt.103018.

    Genome annotation databases

    EnsembliENSBTAT00000038186; ENSBTAP00000038002; ENSBTAG00000004018.
    GeneIDi506348.
    KEGGibta:506348.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC126521 mRNA. Translation: AAI26522.1 .
    RefSeqi NP_001071328.1. NM_001077860.1.
    XP_005206588.1. XM_005206531.1.
    UniGenei Bt.103018.

    3D structure databases

    ProteinModelPortali A0JN54.
    SMRi A0JN54. Positions 1-117.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000038002.

    Proteomic databases

    PRIDEi A0JN54.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000038186 ; ENSBTAP00000038002 ; ENSBTAG00000004018 .
    GeneIDi 506348.
    KEGGi bta:506348.

    Organism-specific databases

    CTDi 1606.

    Phylogenomic databases

    eggNOGi NOG47311.
    GeneTreei ENSGT00750000117569.
    HOGENOMi HOG000252931.
    HOVERGENi HBG051345.
    InParanoidi A0JN54.
    KOi K00901.
    OMAi LVGLHCV.
    OrthoDBi EOG75XGK8.
    TreeFami TF313104.

    Enzyme and pathway databases

    Reactomei REACT_216264. Effects of PIP2 hydrolysis.

    Miscellaneous databases

    NextBioi 20867572.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal skin.

    Entry informationi

    Entry nameiDGKA_BOVIN
    AccessioniPrimary (citable) accession number: A0JN54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3