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Protein

Diacylglycerol kinase alpha

Gene

DGKA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi122 – 133121CuratedAdd
BLAST
Calcium bindingi167 – 178122CuratedAdd
BLAST
Zinc fingeri204 – 25249Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri268 – 31851Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. diacylglycerol kinase activity Source: UniProtKB-EC
  4. NAD+ kinase activity Source: InterPro
  5. phospholipid binding Source: Ensembl

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_304206. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase alpha (EC:2.7.1.107)
Short name:
DAG kinase alpha
Alternative name(s):
Diglyceride kinase alpha
Short name:
DGK-alpha
Gene namesi
Name:DGKA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 5

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. plasma membrane Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 734734Diacylglycerol kinase alphaPRO_0000281914Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei483 – 4831N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiA0JN54.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000038002.

Structurei

3D structure databases

ProteinModelPortaliA0JN54.
SMRiA0JN54. Positions 1-117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini109 – 14436EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini154 – 18936EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini371 – 505135DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri204 – 25249Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri268 – 31851Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiA0JN54.
KOiK00901.
OMAiVGLHCVW.
OrthoDBiEOG75XGK8.
TreeFamiTF313104.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JN54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA EYLQGDAIGY
60 70 80 90 100
EGFQQFLKIY LEVDNVPDHL SQALFQSFQT GYYIEDTVRE DVVCLSDVSC
110 120 130 140 150
YFSLLEGGRP EDKLEFTFKL YDTDRNGILD SSEVDRIIIQ MMRMAEYLDW
160 170 180 190 200
DVSELRPILQ EMMKEIDYDG SGSVSLAEWL RAGATTVPLL VLLGLEMTLK
210 220 230 240 250
DNGQHMWRPK RFPRPVYCNL CESSIGLGKQ GLSCNLCKYI VHDQCAMKAL
260 270 280 290 300
PCEVSTYAKS RKDIGVQSHV WVRGGCESGR CDRCQKKIRI YHSLVGLHCV
310 320 330 340 350
WCHLEIHDDC LPAMGHECDC GLLRDHILPP SSIYPSVLAS GQERKTSKIS
360 370 380 390 400
QKTMDDLSLS TSEALRIDPV SNTHPLLVFV NPKSGGKQGE RVLWKFQYLL
410 420 430 440 450
NPRQVFNLLK DGPEPGLRFF RDVPDYRILV CGGDGTVGWI LESIDKANLP
460 470 480 490 500
FVPPVAVLPL GTGNDLARCL RWGGGYEGQN LGKILKDLET SKVVHMDRWS
510 520 530 540 550
VEVIPQQTEE KSDPVPFQII NNYFSIGVDA SIAHRFHIMR EKYPEKFNSR
560 570 580 590 600
MKNKLWYFEF ATSESIFSTC KKLEESLTVE ICGKPLDLSN LSLEGIAVLN
610 620 630 640 650
IPSTHGGSNL WGDTKRPHGD IHGINQALGA TAKVITDPDI LKTCVPDLSD
660 670 680 690 700
KRLEVVGLEG AIEIGQIYTK LKNAGHRLAK CSEITFHTTK TLPMQIDGEP
710 720 730
WMQTPCTIKI THRNQMPMLV GPPPRSSNFF GFLC
Length:734
Mass (Da):82,672
Last modified:December 11, 2006 - v1
Checksum:i63B02FAC44AB473A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126521 mRNA. Translation: AAI26522.1.
RefSeqiNP_001071328.1. NM_001077860.1.
UniGeneiBt.103018.

Genome annotation databases

EnsembliENSBTAT00000038186; ENSBTAP00000038002; ENSBTAG00000004018.
GeneIDi506348.
KEGGibta:506348.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126521 mRNA. Translation: AAI26522.1.
RefSeqiNP_001071328.1. NM_001077860.1.
UniGeneiBt.103018.

3D structure databases

ProteinModelPortaliA0JN54.
SMRiA0JN54. Positions 1-117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000038002.

Proteomic databases

PRIDEiA0JN54.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000038186; ENSBTAP00000038002; ENSBTAG00000004018.
GeneIDi506348.
KEGGibta:506348.

Organism-specific databases

CTDi1606.

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiA0JN54.
KOiK00901.
OMAiVGLHCVW.
OrthoDBiEOG75XGK8.
TreeFamiTF313104.

Enzyme and pathway databases

ReactomeiREACT_304206. Effects of PIP2 hydrolysis.

Miscellaneous databases

NextBioi20867572.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.

Entry informationi

Entry nameiDGKA_BOVIN
AccessioniPrimary (citable) accession number: A0JN54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 2, 2007
Last sequence update: December 11, 2006
Last modified: March 31, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.