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A0JN52 (SF3B3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Splicing factor 3B subunit 3
Alternative name(s):
Pre-mRNA-splicing factor SF3b 130 kDa subunit
Short name=SF3b130
Spliceosome-associated protein 130
Short name=SAP 130
Gene names
Name:SF3B3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length1217 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Subunit structure

Identified in the spliceosome C complex. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Component of splicing factor SF3B which is composed of at least eight subunits; SF3B1/SAP155/SF3B155, SF3B2/SAP145/SF3B145, SF3B3/SAP130/SF3B130, SF3B4/SAP49/SF3B49, SF3B14A, PHF5A/SF3B14B, SF3B10 and SF3B125. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interaction between SF3B3 and SF3B1 is tighter than the interaction between SF3B3 and SF3B2. Associates with the STAGA transcription coactivator-HAT complex. Interacts with SUPT3H By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the RSE1 family.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
Spliceosome
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA splicing

Inferred from electronic annotation. Source: UniProtKB-KW

mRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentU12-type spliceosomal complex

Inferred from electronic annotation. Source: Compara

catalytic step 2 spliceosome

Inferred from electronic annotation. Source: Compara

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12171217Splicing factor 3B subunit 3
PRO_0000276754

Sequences

Sequence LengthMass (Da)Tools
A0JN52 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 35CACAF5ACC32512

FASTA1,217135,577
        10         20         30         40         50         60 
MFLYNLTLQR ATGISFAIHG NFSGTKQQEI VVSRGKILEL LRPDPNTGKV HTLLTVEVFG 

        70         80         90        100        110        120 
VIRSLMAFRL TGGTKDYIVV GSDSGRIVIL EYQPSKNMFE KIHQETFGKS GCRRIVPGQF 

       130        140        150        160        170        180 
LAVDPKGRAV MISAIEKQKL VYILNRDAAA RLTISSPLEA HKANTLVYHV VGVDVGFENP 

       190        200        210        220        230        240 
MFACLEMDYE EADNDPTGEA AANTQQTLTF YELDLGLNHV VRKYSEPLEE HGNFLITVPG 

       250        260        270        280        290        300 
GSDGPSGVLI CSENYITYKN FGDQPDIRCP IPRRRNDLDD PERGMIFVCS ATHKTKSMFF 

       310        320        330        340        350        360 
FLAQTEQGDI FKITLETDED MVTEIRLKYF DTVPVAAAMC VLKTGFLFVA SEFGNHYLYQ 

       370        380        390        400        410        420 
IAHLGDDDEE PEFSSAMPLE EGDTFFFQPR PLKNLVLVDE LDSLSPILFC QIADLANEDT 

       430        440        450        460        470        480 
PQLYVACGRG PRSSLRVLRH GLEVSEMAVS ELPGNPNAVW TVRRHIEDEF DAYIIVSFVN 

       490        500        510        520        530        540 
ATLVLSIGET VEEVTDSGFL GTTPTLSCSL LGDDALVQVY PDGIRHIRAD KRVNEWKTPG 

       550        560        570        580        590        600 
KKTIVKCAVN QRQVVIALTG GELVYFEMDP SGQLNEYTER KEMSADVVCM SLANVPPGEQ 

       610        620        630        640        650        660 
RSRFLAVGLV DNTVRIISLD PSDCLQPLSM QALPAQPESL CIVEMGGTEK QDELGERGSI 

       670        680        690        700        710        720 
GFLYLNIGLQ NGVLLRTVLD PVTGDLSDTR TRYLGSRPVK LFRVRMQGQE AVLAMSSRSW 

       730        740        750        760        770        780 
LSYSYQSRFH LTPLSYETLE FASGFASEQC PEGIVAISTN TLRILALEKL GAVFNQVAFP 

       790        800        810        820        830        840 
LQYTPRKFVI HPESNNLIII ETDHNAYTEA TKAQRKQQMA EEMVEAAGED ERELAAEMAA 

       850        860        870        880        890        900 
AFLNENLPES IFGAPKAGNG QWASVIRVMN PIQGNTLDLV QLEQNEAAFS VAVCRFSNTG 

       910        920        930        940        950        960 
EDWYVLVGVA KDLILNPRSV AGGFVYTYKL VNNGEKLEFL HKTPVEEVPA AIAPFQGRVL 

       970        980        990       1000       1010       1020 
IGVGKLLRVY DLGKKKLLRK CENKHIANYI SGIQTIGHRV IVSDVQESFI WVRYKRNENQ 

      1030       1040       1050       1060       1070       1080 
LIIFADDTYP RWVTTASLLD YDTVAGADKF GNICVVRLPP NTNDEVDEDP TGNKALWDRG 

      1090       1100       1110       1120       1130       1140 
LLNGASQKAE VIMNYHVGET VLSLQKTTLI PGGSESLVYT TLSGGIGILV PFTSHEDHDF 

      1150       1160       1170       1180       1190       1200 
FQHVEMHLRS EHPPLCGRDH LSFRSYYFPV KNVIDGDLCE QFNSMEPNKQ KNVSEELDRT 

      1210 
PPEVSKKLED IRTRYAF

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References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Brain cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC126518 mRNA. Translation: AAI26519.1.
IPIIPI00690059.
RefSeqNP_001071319.1. NM_001077851.1.
UniGeneBt.7895.

3D structure databases

ProteinModelPortalA0JN52.
ModBaseSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000014050.

Proteomic databases

PaxDbA0JN52.
PRIDEA0JN52.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000014050; ENSBTAP00000014050; ENSBTAG00000010627.
GeneID504962.
KEGGbta:504962.

Organism-specific databases

CTD23450.

Phylogenomic databases

eggNOGNOG247734.
GeneTreeENSGT00530000063396.
HOGENOMHOG000216677.
HOVERGENHBG093942.
InParanoidA0JN52.
KOK12830.
OMAFDTIPVA.
OrthoDBEOG4RV2QJ.

Enzyme and pathway databases

BioCycCATTLE:504962-MONOMER.

Family and domain databases

InterProIPR004871. Cleavage/polyA-sp_fac_asu_C.
[Graphical view]
PfamPF03178. CPSF_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBA0JN52.
NextBio20866909.

Entry information

Entry nameSF3B3_BOVIN
AccessionPrimary (citable) accession number: A0JN52
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: December 12, 2006
Last modified: April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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