Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

General transcription factor IIH subunit 2

Gene

Gtf2h2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri292 – 309C4-typeAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-113418. Formation of the Early Elongation Complex.
R-RNO-5696395. Formation of Incision Complex in GG-NER.
R-RNO-5696400. Dual Incision in GG-NER.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6781823. Formation of TC-NER Pre-Incision Complex.
R-RNO-6782135. Dual incision in TC-NER.
R-RNO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-RNO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-RNO-72086. mRNA Capping.
R-RNO-73762. RNA Polymerase I Transcription Initiation.
R-RNO-73772. RNA Polymerase I Promoter Escape.
R-RNO-73776. RNA Polymerase II Promoter Escape.
R-RNO-73777. RNA Polymerase I Chain Elongation.
R-RNO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-RNO-73863. RNA Polymerase I Transcription Termination.
R-RNO-75953. RNA Polymerase II Transcription Initiation.
R-RNO-75955. RNA Polymerase II Transcription Elongation.
R-RNO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-RNO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 2
Alternative name(s):
Basic transcription factor 2 44 kDa subunit
Short name:
BTF2 p44
General transcription factor IIH polypeptide 2
TFIIH basal transcription factor complex p44 subunit
Gene namesi
Name:Gtf2h2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1310499. Gtf2h2.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003275661 – 396General transcription factor IIH subunit 2Add BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei95PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA0JN27.
PeptideAtlasiA0JN27.
PRIDEiA0JN27.

PTM databases

iPTMnetiA0JN27.
PhosphoSitePlusiA0JN27.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018230.
GenevisibleiA0JN27. RN.

Interactioni

Subunit structurei

Component of the TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2 and ERCC3. One of the 6 subunits forming the core-TFIIH basal transcription factor. The core-TFIIH basal transcription factor associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with XPB, XPD, GTF2H1 and GTF2H3. Interacts with varicella-zoster virus IE63 protein.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057895.

Structurei

3D structure databases

ProteinModelPortaliA0JN27.
SMRiA0JN27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 236VWFAPROSITE-ProRule annotationAdd BLAST177

Sequence similaritiesi

Belongs to the GTF2H2 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri292 – 309C4-typeAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2807. Eukaryota.
COG5151. LUCA.
GeneTreeiENSGT00490000043395.
HOGENOMiHOG000159415.
HOVERGENiHBG059468.
InParanoidiA0JN27.
KOiK03142.
OMAiDCDIFIH.
OrthoDBiEOG091G08ZR.
PhylomeDBiA0JN27.
TreeFamiTF314037.

Family and domain databases

CDDicd01453. vWA_transcription_factor_IIH_t. 1 hit.
Gene3Di3.40.50.410. 1 hit.
InterProiView protein in InterPro
IPR007198. Ssl1-like.
IPR004595. TFIIH_C1-like_dom.
IPR012170. TFIIH_SSL1/p44.
IPR002035. VWF_A.
IPR013087. Znf_C2H2_type.
PANTHERiPTHR12695:SF3. PTHR12695:SF3. 1 hit.
PfamiView protein in Pfam
PF07975. C1_4. 1 hit.
PF04056. Ssl1. 1 hit.
PIRSFiPIRSF015919. TFIIH_SSL1. 1 hit.
SMARTiView protein in SMART
SM01047. C1_4. 1 hit.
SM00327. VWA. 1 hit.
SUPFAMiSSF53300. SSF53300. 1 hit.
TIGRFAMsiTIGR00622. ssl1. 1 hit.
PROSITEiView protein in PROSITE
PS50234. VWFA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0JN27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEEPERTKR WEGGYERTWE ILKEDESGSL KATIEDILFK AKRKRVFEHH
60 70 80 90 100
GQVRLGMMRH LYVVVDGSRT MEDQDLKPNR LTCTLKLLEY FVEEYFDQNP
110 120 130 140 150
ISQIGIIVTK SKRAEKLTEL SGNPRKHITS LKKAVDMTCH GEPSLYNSLS
160 170 180 190 200
MAMQTLKHMP GHTSREVLII FSSLTTCDPS NIYDLIKTLK TAKIRVSVIG
210 220 230 240 250
LSAEVRVCTV LARETGGTYH VILDETHYKE LLARHVSPPP ASSGSECSLI
260 270 280 290 300
RMGFPQHTIA SLSDQDAKPS FSMAHLDNNS TEPGLTLGGY FCPQCRAKYC
310 320 330 340 350
ELPVECKICG LTLVSAPHLA RSYHHLFPLD AFQEIPLEEY KGERFCYGCQ
360 370 380 390
GELKDQHVYV CTVCRNVFCV DCDVFVHDSL HCCPGCVHKI PTQSGV
Length:396
Mass (Da):44,703
Last modified:December 12, 2006 - v1
Checksum:i2812ED7B0DC5B3C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126097 mRNA. Translation: AAI26098.1.
RefSeqiNP_001070896.1. NM_001077428.1.
XP_006231906.1. XM_006231844.3.
XP_006231907.1. XM_006231845.3.
UniGeneiRn.144260.

Genome annotation databases

EnsembliENSRNOT00000061183; ENSRNOP00000057895; ENSRNOG00000018230.
GeneIDi294693.
KEGGirno:294693.
UCSCiRGD:1310499. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTF2H2_RAT
AccessioniPrimary (citable) accession number: A0JN27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: December 12, 2006
Last modified: June 7, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families