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Protein

General transcription factor IIH subunit 2

Gene

Gtf2h2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri292 – 30918C4-typeAdd
BLAST

GO - Molecular functioni

  1. zinc ion binding Source: InterPro

GO - Biological processi

  1. nucleotide-excision repair Source: InterPro
  2. protein phosphorylation Source: GOC
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_271824. RNA Polymerase II Promoter Escape.
REACT_273293. RNA Polymerase II Pre-transcription Events.
REACT_280841. RNA Polymerase I Transcription Initiation.
REACT_283793. Formation of RNA Pol II elongation complex.
REACT_290439. Dual incision reaction in GG-NER.
REACT_290496. RNA Polymerase I Chain Elongation.
REACT_291085. RNA Polymerase I Transcription Termination.
REACT_297910. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_319445. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_323062. mRNA Capping.
REACT_323975. Dual incision reaction in TC-NER.
REACT_332743. Formation of incision complex in GG-NER.
REACT_338490. RNA Polymerase I Promoter Escape.
REACT_340454. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_340969. Formation of the Early Elongation Complex.
REACT_343091. RNA Polymerase II Transcription Elongation.
REACT_343213. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_351677. RNA Polymerase II Transcription Initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 2
Alternative name(s):
Basic transcription factor 2 44 kDa subunit
Short name:
BTF2 p44
General transcription factor IIH polypeptide 2
TFIIH basal transcription factor complex p44 subunit
Gene namesi
Name:Gtf2h2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1310499. Gtf2h2.

Subcellular locationi

  1. Nucleus By similarity

GO - Cellular componenti

  1. core TFIIH complex Source: InterPro
  2. holo TFIIH complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396General transcription factor IIH subunit 2PRO_0000327566Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei95 – 951Phosphotyrosine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA0JN27.
PRIDEiA0JN27.

PTM databases

PhosphoSiteiA0JN27.

Expressioni

Gene expression databases

GenevestigatoriA0JN27.

Interactioni

Subunit structurei

One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with XPB, XPD, GTF2H1 and GTF2H3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057895.

Structurei

3D structure databases

ProteinModelPortaliA0JN27.
SMRiA0JN27. Positions 329-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 236177VWFAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GTF2H2 family.Curated
Contains 1 VWFA domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri292 – 30918C4-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5151.
GeneTreeiENSGT00490000043395.
HOGENOMiHOG000159415.
HOVERGENiHBG059468.
InParanoidiA0JN27.
KOiK03142.
OMAiECKVCGL.
OrthoDBiEOG7WQ7SP.
PhylomeDBiA0JN27.
TreeFamiTF314037.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR007198. Ssl1-like.
IPR004595. TFIIH_C1-like_dom.
IPR012170. TFIIH_SSL1/p44.
IPR002035. VWF_A.
IPR007087. Znf_C2H2.
[Graphical view]
PANTHERiPTHR12695:SF2. PTHR12695:SF2. 1 hit.
PfamiPF07975. C1_4. 1 hit.
PF04056. Ssl1. 1 hit.
[Graphical view]
PIRSFiPIRSF015919. TFIIH_SSL1. 1 hit.
SMARTiSM01047. C1_4. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
TIGRFAMsiTIGR00622. ssl1. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JN27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEEPERTKR WEGGYERTWE ILKEDESGSL KATIEDILFK AKRKRVFEHH
60 70 80 90 100
GQVRLGMMRH LYVVVDGSRT MEDQDLKPNR LTCTLKLLEY FVEEYFDQNP
110 120 130 140 150
ISQIGIIVTK SKRAEKLTEL SGNPRKHITS LKKAVDMTCH GEPSLYNSLS
160 170 180 190 200
MAMQTLKHMP GHTSREVLII FSSLTTCDPS NIYDLIKTLK TAKIRVSVIG
210 220 230 240 250
LSAEVRVCTV LARETGGTYH VILDETHYKE LLARHVSPPP ASSGSECSLI
260 270 280 290 300
RMGFPQHTIA SLSDQDAKPS FSMAHLDNNS TEPGLTLGGY FCPQCRAKYC
310 320 330 340 350
ELPVECKICG LTLVSAPHLA RSYHHLFPLD AFQEIPLEEY KGERFCYGCQ
360 370 380 390
GELKDQHVYV CTVCRNVFCV DCDVFVHDSL HCCPGCVHKI PTQSGV
Length:396
Mass (Da):44,703
Last modified:December 12, 2006 - v1
Checksum:i2812ED7B0DC5B3C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126097 mRNA. Translation: AAI26098.1.
RefSeqiNP_001070896.1. NM_001077428.1.
XP_006231906.1. XM_006231844.2.
XP_006231907.1. XM_006231845.2.
UniGeneiRn.144260.

Genome annotation databases

EnsembliENSRNOT00000061183; ENSRNOP00000057895; ENSRNOG00000018230.
GeneIDi294693.
KEGGirno:294693.
UCSCiRGD:1310499. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC126097 mRNA. Translation: AAI26098.1.
RefSeqiNP_001070896.1. NM_001077428.1.
XP_006231906.1. XM_006231844.2.
XP_006231907.1. XM_006231845.2.
UniGeneiRn.144260.

3D structure databases

ProteinModelPortaliA0JN27.
SMRiA0JN27. Positions 329-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057895.

PTM databases

PhosphoSiteiA0JN27.

Proteomic databases

PaxDbiA0JN27.
PRIDEiA0JN27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000061183; ENSRNOP00000057895; ENSRNOG00000018230.
GeneIDi294693.
KEGGirno:294693.
UCSCiRGD:1310499. rat.

Organism-specific databases

CTDi2966.
RGDi1310499. Gtf2h2.

Phylogenomic databases

eggNOGiCOG5151.
GeneTreeiENSGT00490000043395.
HOGENOMiHOG000159415.
HOVERGENiHBG059468.
InParanoidiA0JN27.
KOiK03142.
OMAiECKVCGL.
OrthoDBiEOG7WQ7SP.
PhylomeDBiA0JN27.
TreeFamiTF314037.

Enzyme and pathway databases

ReactomeiREACT_271824. RNA Polymerase II Promoter Escape.
REACT_273293. RNA Polymerase II Pre-transcription Events.
REACT_280841. RNA Polymerase I Transcription Initiation.
REACT_283793. Formation of RNA Pol II elongation complex.
REACT_290439. Dual incision reaction in GG-NER.
REACT_290496. RNA Polymerase I Chain Elongation.
REACT_291085. RNA Polymerase I Transcription Termination.
REACT_297910. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_319445. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_323062. mRNA Capping.
REACT_323975. Dual incision reaction in TC-NER.
REACT_332743. Formation of incision complex in GG-NER.
REACT_338490. RNA Polymerase I Promoter Escape.
REACT_340454. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_340969. Formation of the Early Elongation Complex.
REACT_343091. RNA Polymerase II Transcription Elongation.
REACT_343213. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_351677. RNA Polymerase II Transcription Initiation.

Miscellaneous databases

NextBioi638415.
PROiA0JN27.

Gene expression databases

GenevestigatoriA0JN27.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR007198. Ssl1-like.
IPR004595. TFIIH_C1-like_dom.
IPR012170. TFIIH_SSL1/p44.
IPR002035. VWF_A.
IPR007087. Znf_C2H2.
[Graphical view]
PANTHERiPTHR12695:SF2. PTHR12695:SF2. 1 hit.
PfamiPF07975. C1_4. 1 hit.
PF04056. Ssl1. 1 hit.
[Graphical view]
PIRSFiPIRSF015919. TFIIH_SSL1. 1 hit.
SMARTiSM01047. C1_4. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
TIGRFAMsiTIGR00622. ssl1. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTF2H2_RAT
AccessioniPrimary (citable) accession number: A0JN27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: December 12, 2006
Last modified: April 1, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.