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A0JMQ9

- ZRN1B_DANRE

UniProt

A0JMQ9 - ZRN1B_DANRE

Protein

Ubiquitin thioesterase zranb1-B

Gene

zranb1b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. May play a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei451 – 4511NucleophileBy similarity
    Active sitei593 – 5931Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri82 – 11130RanBP2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri152 – 18130RanBP2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB
    2. ubiquitin thiolesterase activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell migration Source: UniProtKB
    2. central nervous system morphogenesis Source: ZFIN
    3. cytoskeleton organization Source: UniProtKB
    4. positive regulation of Wnt signaling pathway Source: UniProtKB
    5. protein K29-linked deubiquitination Source: UniProtKB
    6. protein K33-linked deubiquitination Source: UniProtKB
    7. protein K63-linked deubiquitination Source: UniProtKB
    8. regulation of cell morphogenesis Source: UniProtKB
    9. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway, Wnt signaling pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC64.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase zranb1-B (EC:3.4.19.12)
    Alternative name(s):
    Zinc finger Ran-binding domain-containing protein 1-B
    Gene namesi
    Name:zranb1b
    Synonyms:zranb1
    ORF Names:zgc:154177
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 17

    Organism-specific databases

    ZFINiZDB-GENE-061110-94. zranb1b.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 716716Ubiquitin thioesterase zranb1-BPRO_0000361555Add
    BLAST

    Proteomic databases

    PRIDEiA0JMQ9.

    Expressioni

    Gene expression databases

    BgeeiA0JMQ9.

    Interactioni

    Protein-protein interaction databases

    BioGridi607517. 3 interactions.
    STRINGi7955.ENSDARP00000086546.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati268 – 29831ANK 1Add
    BLAST
    Repeati321 – 34828ANK 2Add
    BLAST
    Domaini440 – 600161OTUPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi214 – 2196Poly-Gly
    Compositional biasi708 – 7169Poly-Glu

    Domaini

    The OTU domain mediates the deubiquitinating activity.By similarity
    The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.By similarity

    Sequence similaritiesi

    Belongs to the peptidase C64 family.Curated
    Contains 2 ANK repeats.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation
    Contains 3 RanBP2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri82 – 11130RanBP2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri152 – 18130RanBP2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    ANK repeat, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG253754.
    GeneTreeiENSGT00530000062989.
    HOGENOMiHOG000006743.
    HOVERGENiHBG058978.
    InParanoidiA0JMQ9.
    KOiK11862.
    OMAiHERDDIL.
    OrthoDBiEOG7R56SB.
    PhylomeDBiA0JMQ9.
    TreeFamiTF323312.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR001876. Znf_RanBP2.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    PF00641. zf-RanBP. 1 hit.
    [Graphical view]
    SMARTiSM00547. ZnF_RBZ. 3 hits.
    [Graphical view]
    PROSITEiPS50802. OTU. 1 hit.
    PS01358. ZF_RANBP2_1. 3 hits.
    PS50199. ZF_RANBP2_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0JMQ9-1 [UniParc]FASTAAdd to Basket

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    MTDLGLKWSC EYCTYENWPS AIKCTMCRAQ RHNAPIITEE PFKSSSSLDP    50
    SLCTTQGGST LLICPDSSAR PRVRIADELP ETSSKWSCHM CTYLNWPRAI 100
    RCTQCLSQRQ QGSQQHSPLS PSETPQTSGS RPSPVTSDPC EEYNDRNRLN 150
    MHAQRWPCSA CTYENWPKSL RCVVCDHPKP SGSPETPQQD SEAESATSPS 200
    IVNEQERENV RTAGGGGGGS RGRLRKLSPP MCKGQAEVKI ELASGAVGSD 250
    NEQEADFKKL KQIRNRMRRS DWLFLNACAG VVEGDLAAVE AYKSSGGDIA 300
    RQLTADEVRI LNRPSAFDAG FTLVHLAIRF QRQDMLAVLL TEVSQQTAKC 350
    IPALVCPELT EQIRREVAAA LHRRKGEFPC YFFTDLVTFT LPADIEDLPP 400
    NVQEKLFDEV LDRDVQKELE EESPIINWSL ELGTRLDSRL YALWNRTAGD 450
    CLLDSVLQAT WGIYDKDSVL RKSLNDSLHD CSHWFYTRWK EWESWYSQSF 500
    GLHFSLREEQ WQEDWAFILS LASQPGASLE QTHVFVLAHI LRRPIIVYGV 550
    KYYKSFRGET LGYTRFQGVY LPLLWEQSFC WKSPIALGYT RGHFSALVAM 600
    ENDGYDNRGA GANLNTDDDV TVTFLPLVDS ERKLLHIHFL SAQEMGTEEQ 650
    QERMLRQWMD CCVTEGGVLV AMQKSSRRRN HPLVTQMVEK WLDGYRQLAA 700
    CPTLSDGEEE EEDEDE 716
    Length:716
    Mass (Da):81,193
    Last modified:December 12, 2006 - v1
    Checksum:iE18EB538F055BB75
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC125972 mRNA. Translation: AAI25973.1.
    RefSeqiNP_001071236.1. NM_001077768.1.
    XP_005158652.1. XM_005158595.1.
    UniGeneiDr.118787.

    Genome annotation databases

    EnsembliENSDART00000092113; ENSDARP00000086546; ENSDARG00000063190.
    GeneIDi777720.
    KEGGidre:777720.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC125972 mRNA. Translation: AAI25973.1 .
    RefSeqi NP_001071236.1. NM_001077768.1.
    XP_005158652.1. XM_005158595.1.
    UniGenei Dr.118787.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 607517. 3 interactions.
    STRINGi 7955.ENSDARP00000086546.

    Protein family/group databases

    MEROPSi C64.004.

    Proteomic databases

    PRIDEi A0JMQ9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000092113 ; ENSDARP00000086546 ; ENSDARG00000063190 .
    GeneIDi 777720.
    KEGGi dre:777720.

    Organism-specific databases

    CTDi 777720.
    ZFINi ZDB-GENE-061110-94. zranb1b.

    Phylogenomic databases

    eggNOGi NOG253754.
    GeneTreei ENSGT00530000062989.
    HOGENOMi HOG000006743.
    HOVERGENi HBG058978.
    InParanoidi A0JMQ9.
    KOi K11862.
    OMAi HERDDIL.
    OrthoDBi EOG7R56SB.
    PhylomeDBi A0JMQ9.
    TreeFami TF323312.

    Miscellaneous databases

    NextBioi 20924285.

    Gene expression databases

    Bgeei A0JMQ9.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR001876. Znf_RanBP2.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    PF00641. zf-RanBP. 1 hit.
    [Graphical view ]
    SMARTi SM00547. ZnF_RBZ. 3 hits.
    [Graphical view ]
    PROSITEi PS50802. OTU. 1 hit.
    PS01358. ZF_RANBP2_1. 3 hits.
    PS50199. ZF_RANBP2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiZRN1B_DANRE
    AccessioniPrimary (citable) accession number: A0JMQ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3