A0JMQ9 (ZRN1B_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase zranb1-B
EC=3.4.19.12

Alternative name(s):
Zinc finger Ran-binding domain-containing protein 1-B

Gene names

Name:	zranb1b
Synonyms:	zranb1
ORF Names:	zgc:154177

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. May play a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	The OTU domain mediates the deubiquitinating activity By similarity. The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin By similarity.
Sequence similarities	Belongs to the peptidase C64 family. Contains 2 ANK repeats. Contains 1 OTU domain. Contains 3 RanBP2-type zinc fingers.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Wnt signaling pathway
Cellular component	Cytoplasm Nucleus
Domain	ANK repeat Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell migration Inferred from sequence or structural similarity. Source: UniProtKB central nervous system morphogenesis Inferred from mutant phenotype PubMed 20040115. Source: ZFIN cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 716	716	Ubiquitin thioesterase zranb1-B		PRO_0000361555
Regions
	Repeat	268 – 298	31	ANK 1
	Repeat	321 – 348	28	ANK 2
	Domain	440 – 600	161	OTU
	Zinc finger	3 – 33	31	RanBP2-type 1
	Zinc finger	82 – 111	30	RanBP2-type 2
	Zinc finger	152 – 181	30	RanBP2-type 3
	Compositional bias	214 – 219	6	Poly-Gly
	Compositional bias	708 – 716	9	Poly-Glu
Sites
	Active site	451	1	Nucleophile By similarity
	Active site	593	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0JMQ9 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: E18EB538F055BB75	FASTA	716	81,193
	10 20 30 40 50 60 MTDLGLKWSC EYCTYENWPS AIKCTMCRAQ RHNAPIITEE PFKSSSSLDP SLCTTQGGST 70 80 90 100 110 120 LLICPDSSAR PRVRIADELP ETSSKWSCHM CTYLNWPRAI RCTQCLSQRQ QGSQQHSPLS 130 140 150 160 170 180 PSETPQTSGS RPSPVTSDPC EEYNDRNRLN MHAQRWPCSA CTYENWPKSL RCVVCDHPKP 190 200 210 220 230 240 SGSPETPQQD SEAESATSPS IVNEQERENV RTAGGGGGGS RGRLRKLSPP MCKGQAEVKI 250 260 270 280 290 300 ELASGAVGSD NEQEADFKKL KQIRNRMRRS DWLFLNACAG VVEGDLAAVE AYKSSGGDIA 310 320 330 340 350 360 RQLTADEVRI LNRPSAFDAG FTLVHLAIRF QRQDMLAVLL TEVSQQTAKC IPALVCPELT 370 380 390 400 410 420 EQIRREVAAA LHRRKGEFPC YFFTDLVTFT LPADIEDLPP NVQEKLFDEV LDRDVQKELE 430 440 450 460 470 480 EESPIINWSL ELGTRLDSRL YALWNRTAGD CLLDSVLQAT WGIYDKDSVL RKSLNDSLHD 490 500 510 520 530 540 CSHWFYTRWK EWESWYSQSF GLHFSLREEQ WQEDWAFILS LASQPGASLE QTHVFVLAHI 550 560 570 580 590 600 LRRPIIVYGV KYYKSFRGET LGYTRFQGVY LPLLWEQSFC WKSPIALGYT RGHFSALVAM 610 620 630 640 650 660 ENDGYDNRGA GANLNTDDDV TVTFLPLVDS ERKLLHIHFL SAQEMGTEEQ QERMLRQWMD 670 680 690 700 710 CCVTEGGVLV AMQKSSRRRN HPLVTQMVEK WLDGYRQLAA CPTLSDGEEE EEDEDE

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC125972 mRNA. Translation: AAI25973.1.
IPI	IPI00817674.
RefSeq	NP_001071236.1. NM_001077768.1.
UniGene	Dr.118787.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	C64.004.
Proteomic databases
PRIDE	A0JMQ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000092113; ENSDARP00000086546; ENSDARG00000063190.
GeneID	777720.
KEGG	dre:777720.
Organism-specific databases
CTD	777720.
ZFIN	ZDB-GENE-061110-94. zranb1b.
Phylogenomic databases
eggNOG	NOG253754.
GeneTree	ENSGT00530000062989.
HOGENOM	HOG000006743.
HOVERGEN	HBG058978.
InParanoid	A0JMQ9.
KO	K11862.
OMA	EIGNEEQ.
OrthoDB	EOG441Q9X.
Gene expression databases
Bgee	A0JMQ9.
Family and domain databases
InterPro	IPR003323. OTU. IPR001876. Znf_RanBP2. [Graphical view]
Pfam	PF02338. OTU. 1 hit. PF00641. zf-RanBP. 1 hit. [Graphical view]
SMART	SM00547. ZnF_RBZ. 3 hits. [Graphical view]
PROSITE	PS50088. ANK_REPEAT. False negative. PS50802. OTU. 1 hit. PS01358. ZF_RANBP2_1. 3 hits. PS50199. ZF_RANBP2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20924285.

Entry information

Entry name

ZRN1B_DANRE

Accession

Primary (citable) accession number: A0JMQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	December 12, 2006
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program