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A0JMQ9

- ZRN1B_DANRE

UniProt

A0JMQ9 - ZRN1B_DANRE

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Protein

Ubiquitin thioesterase zranb1-B

Gene

zranb1b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. May play a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei451 – 4511NucleophileBy similarity
Active sitei593 – 5931Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri82 – 11130RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri152 – 18130RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB
  2. ubiquitin thiolesterase activity Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell migration Source: UniProtKB
  2. central nervous system morphogenesis Source: ZFIN
  3. cytoskeleton organization Source: UniProtKB
  4. positive regulation of Wnt signaling pathway Source: UniProtKB
  5. protein K29-linked deubiquitination Source: UniProtKB
  6. protein K33-linked deubiquitination Source: UniProtKB
  7. protein K63-linked deubiquitination Source: UniProtKB
  8. regulation of cell morphogenesis Source: UniProtKB
  9. Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway, Wnt signaling pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC64.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase zranb1-B (EC:3.4.19.12)
Alternative name(s):
Zinc finger Ran-binding domain-containing protein 1-B
Gene namesi
Name:zranb1b
Synonyms:zranb1
ORF Names:zgc:154177
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 17

Organism-specific databases

ZFINiZDB-GENE-061110-94. zranb1b.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Ubiquitin thioesterase zranb1-BPRO_0000361555Add
BLAST

Proteomic databases

PRIDEiA0JMQ9.

Expressioni

Gene expression databases

BgeeiA0JMQ9.

Interactioni

Protein-protein interaction databases

BioGridi607517. 3 interactions.
STRINGi7955.ENSDARP00000086546.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati268 – 29831ANK 1Add
BLAST
Repeati321 – 34828ANK 2Add
BLAST
Domaini440 – 600161OTUPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi214 – 2196Poly-Gly
Compositional biasi708 – 7169Poly-Glu

Domaini

The OTU domain mediates the deubiquitinating activity.By similarity
The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.By similarity

Sequence similaritiesi

Belongs to the peptidase C64 family.Curated
Contains 2 ANK repeats.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation
Contains 3 RanBP2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri82 – 11130RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri152 – 18130RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG253754.
GeneTreeiENSGT00530000062989.
HOGENOMiHOG000006743.
HOVERGENiHBG058978.
InParanoidiA0JMQ9.
KOiK11862.
OMAiHERDDIL.
OrthoDBiEOG7R56SB.
PhylomeDBiA0JMQ9.
TreeFamiTF323312.

Family and domain databases

InterProiIPR003323. OTU.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 3 hits.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JMQ9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDLGLKWSC EYCTYENWPS AIKCTMCRAQ RHNAPIITEE PFKSSSSLDP
60 70 80 90 100
SLCTTQGGST LLICPDSSAR PRVRIADELP ETSSKWSCHM CTYLNWPRAI
110 120 130 140 150
RCTQCLSQRQ QGSQQHSPLS PSETPQTSGS RPSPVTSDPC EEYNDRNRLN
160 170 180 190 200
MHAQRWPCSA CTYENWPKSL RCVVCDHPKP SGSPETPQQD SEAESATSPS
210 220 230 240 250
IVNEQERENV RTAGGGGGGS RGRLRKLSPP MCKGQAEVKI ELASGAVGSD
260 270 280 290 300
NEQEADFKKL KQIRNRMRRS DWLFLNACAG VVEGDLAAVE AYKSSGGDIA
310 320 330 340 350
RQLTADEVRI LNRPSAFDAG FTLVHLAIRF QRQDMLAVLL TEVSQQTAKC
360 370 380 390 400
IPALVCPELT EQIRREVAAA LHRRKGEFPC YFFTDLVTFT LPADIEDLPP
410 420 430 440 450
NVQEKLFDEV LDRDVQKELE EESPIINWSL ELGTRLDSRL YALWNRTAGD
460 470 480 490 500
CLLDSVLQAT WGIYDKDSVL RKSLNDSLHD CSHWFYTRWK EWESWYSQSF
510 520 530 540 550
GLHFSLREEQ WQEDWAFILS LASQPGASLE QTHVFVLAHI LRRPIIVYGV
560 570 580 590 600
KYYKSFRGET LGYTRFQGVY LPLLWEQSFC WKSPIALGYT RGHFSALVAM
610 620 630 640 650
ENDGYDNRGA GANLNTDDDV TVTFLPLVDS ERKLLHIHFL SAQEMGTEEQ
660 670 680 690 700
QERMLRQWMD CCVTEGGVLV AMQKSSRRRN HPLVTQMVEK WLDGYRQLAA
710
CPTLSDGEEE EEDEDE
Length:716
Mass (Da):81,193
Last modified:December 12, 2006 - v1
Checksum:iE18EB538F055BB75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC125972 mRNA. Translation: AAI25973.1.
RefSeqiNP_001071236.1. NM_001077768.1.
XP_005158652.1. XM_005158595.2.
UniGeneiDr.118787.

Genome annotation databases

EnsembliENSDART00000092113; ENSDARP00000086546; ENSDARG00000063190.
GeneIDi777720.
KEGGidre:777720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC125972 mRNA. Translation: AAI25973.1 .
RefSeqi NP_001071236.1. NM_001077768.1.
XP_005158652.1. XM_005158595.2.
UniGenei Dr.118787.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 607517. 3 interactions.
STRINGi 7955.ENSDARP00000086546.

Protein family/group databases

MEROPSi C64.004.

Proteomic databases

PRIDEi A0JMQ9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000092113 ; ENSDARP00000086546 ; ENSDARG00000063190 .
GeneIDi 777720.
KEGGi dre:777720.

Organism-specific databases

CTDi 777720.
ZFINi ZDB-GENE-061110-94. zranb1b.

Phylogenomic databases

eggNOGi NOG253754.
GeneTreei ENSGT00530000062989.
HOGENOMi HOG000006743.
HOVERGENi HBG058978.
InParanoidi A0JMQ9.
KOi K11862.
OMAi HERDDIL.
OrthoDBi EOG7R56SB.
PhylomeDBi A0JMQ9.
TreeFami TF323312.

Miscellaneous databases

NextBioi 20924285.

Gene expression databases

Bgeei A0JMQ9.

Family and domain databases

InterProi IPR003323. OTU.
IPR001876. Znf_RanBP2.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view ]
SMARTi SM00547. ZnF_RBZ. 3 hits.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiZRN1B_DANRE
AccessioniPrimary (citable) accession number: A0JMQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: December 12, 2006
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3