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A0JMQ9 (ZRN1B_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin thioesterase zranb1-B

EC=3.4.19.12
Alternative name(s):
Zinc finger Ran-binding domain-containing protein 1-B
Gene names
Name:zranb1b
Synonyms:zranb1
ORF Names:zgc:154177
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length716 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. May play a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Domain

The OTU domain mediates the deubiquitinating activity By similarity.

The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin By similarity.

Sequence similarities

Belongs to the peptidase C64 family.

Contains 2 ANK repeats.

Contains 1 OTU domain.

Contains 3 RanBP2-type zinc fingers.

Ontologies

Keywords
   Biological processUbl conjugation pathway
Wnt signaling pathway
   Cellular componentCytoplasm
Nucleus
   DomainANK repeat
Repeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

central nervous system morphogenesis

Inferred from mutant phenotype PubMed 20040115. Source: ZFIN

cytoskeleton organization

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of Wnt signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

protein K29-linked deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K33-linked deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K63-linked deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of cell morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionubiquitin thiolesterase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-specific protease activity

Inferred from sequence or structural similarity. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 716716Ubiquitin thioesterase zranb1-B
PRO_0000361555

Regions

Repeat268 – 29831ANK 1
Repeat321 – 34828ANK 2
Domain440 – 600161OTU
Zinc finger3 – 3331RanBP2-type 1
Zinc finger82 – 11130RanBP2-type 2
Zinc finger152 – 18130RanBP2-type 3
Compositional bias214 – 2196Poly-Gly
Compositional bias708 – 7169Poly-Glu

Sites

Active site4511Nucleophile By similarity
Active site5931Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
A0JMQ9 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: E18EB538F055BB75

FASTA71681,193
        10         20         30         40         50         60 
MTDLGLKWSC EYCTYENWPS AIKCTMCRAQ RHNAPIITEE PFKSSSSLDP SLCTTQGGST 

        70         80         90        100        110        120 
LLICPDSSAR PRVRIADELP ETSSKWSCHM CTYLNWPRAI RCTQCLSQRQ QGSQQHSPLS 

       130        140        150        160        170        180 
PSETPQTSGS RPSPVTSDPC EEYNDRNRLN MHAQRWPCSA CTYENWPKSL RCVVCDHPKP 

       190        200        210        220        230        240 
SGSPETPQQD SEAESATSPS IVNEQERENV RTAGGGGGGS RGRLRKLSPP MCKGQAEVKI 

       250        260        270        280        290        300 
ELASGAVGSD NEQEADFKKL KQIRNRMRRS DWLFLNACAG VVEGDLAAVE AYKSSGGDIA 

       310        320        330        340        350        360 
RQLTADEVRI LNRPSAFDAG FTLVHLAIRF QRQDMLAVLL TEVSQQTAKC IPALVCPELT 

       370        380        390        400        410        420 
EQIRREVAAA LHRRKGEFPC YFFTDLVTFT LPADIEDLPP NVQEKLFDEV LDRDVQKELE 

       430        440        450        460        470        480 
EESPIINWSL ELGTRLDSRL YALWNRTAGD CLLDSVLQAT WGIYDKDSVL RKSLNDSLHD 

       490        500        510        520        530        540 
CSHWFYTRWK EWESWYSQSF GLHFSLREEQ WQEDWAFILS LASQPGASLE QTHVFVLAHI 

       550        560        570        580        590        600 
LRRPIIVYGV KYYKSFRGET LGYTRFQGVY LPLLWEQSFC WKSPIALGYT RGHFSALVAM 

       610        620        630        640        650        660 
ENDGYDNRGA GANLNTDDDV TVTFLPLVDS ERKLLHIHFL SAQEMGTEEQ QERMLRQWMD 

       670        680        690        700        710 
CCVTEGGVLV AMQKSSRRRN HPLVTQMVEK WLDGYRQLAA CPTLSDGEEE EEDEDE 

« Hide

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC125972 mRNA. Translation: AAI25973.1.
RefSeqNP_001071236.1. NM_001077768.1.
XP_005158652.1. XM_005158595.1.
UniGeneDr.118787.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000086546.

Protein family/group databases

MEROPSC64.004.

Proteomic databases

PRIDEA0JMQ9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000092113; ENSDARP00000086546; ENSDARG00000063190.
GeneID777720.
KEGGdre:777720.

Organism-specific databases

CTD777720.
ZFINZDB-GENE-061110-94. zranb1b.

Phylogenomic databases

eggNOGNOG253754.
GeneTreeENSGT00530000062989.
HOGENOMHOG000006743.
HOVERGENHBG058978.
InParanoidA0JMQ9.
KOK11862.
OMAGHEENIL.
OrthoDBEOG7R56SB.
PhylomeDBA0JMQ9.
TreeFamTF323312.

Gene expression databases

BgeeA0JMQ9.

Family and domain databases

InterProIPR003323. OTU.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamPF02338. OTU. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTSM00547. ZnF_RBZ. 3 hits.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20924285.

Entry information

Entry nameZRN1B_DANRE
AccessionPrimary (citable) accession number: A0JMQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries