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A0JM59 (UBP20_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 20

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 20
Ubiquitin thioesterase 20
Ubiquitin-specific-processing protease 20
Gene names
Name:usp20
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length884 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Deubiquitinating enzyme involved in beta-2 adrenergic receptor (adrb2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (adrb2). Plays a central role in adrb2 recycling and resensitization after prolonged agonist stimulation by constitutively binding adrb2, mediating deubiquitination of adrb2 and inhibiting lysosomal trafficking of adrb2. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity.

Domain

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.

Sequence similarities

Belongs to the peptidase C19 family. USP20/USP33 subfamily.

Contains 2 DUSP domains.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processEndocytosis
Ubl conjugation pathway
   Cellular componentCytoplasm
Cytoskeleton
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processendocytosis

Inferred from electronic annotation. Source: UniProtKB-KW

protein K48-linked deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K63-linked deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of G-protein coupled receptor protein signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcentrosome

Inferred from sequence or structural similarity. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncysteine-type endopeptidase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin thiolesterase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-specific protease activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 884884Ubiquitin carboxyl-terminal hydrolase 20
PRO_0000390422

Regions

Domain145 – 655511USP
Domain657 – 75094DUSP 1
Domain759 – 862104DUSP 2
Zinc finger28 – 9265UBP-type

Sites

Active site1541Nucleophile By similarity
Active site6131Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
A0JM59 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: DA651CCD8694FFF1

FASTA88499,135
        10         20         30         40         50         60 
MGDAEDFCPH LDSIGEVTKE DLILKSKGTC ESCGVGGPNL WACLQDGCQS VGCGESYVDH 

        70         80         90        100        110        120 
STLHAQAKKH NLTVNLTTFR VWCYACEKEV FLDPRGPPAS QTTSPRLSHR DFPTSAHPLK 

       130        140        150        160        170        180 
SVPIAVGDDG ESESDEDDIK PRGLTGMKNI GNSCYMNAAL QALSNCPPLT QFFLECGGLV 

       190        200        210        220        230        240 
RTDKKPALCK SYQKLISELW HKKRPSYVVP SSLYHGIKLI NPLFRGYSQQ DTQEFLRCLM 

       250        260        270        280        290        300 
DQLHEELKEP VPLETQEREE EDRDDQREGE RGGTVEEDFL SCDSGGEMGD GEGGGGVGTL 

       310        320        330        340        350        360 
SEMELLIREE VGRGLSEKEK LKERKLSYCH RRTSSEQADE DADVDTAMIP EPDNDAYVHC 

       370        380        390        400        410        420 
SSRSCSPHPV ESISKHSSTP PRSSPLRTSH SYVLKKAQVL SGGKKRSEVR YRSVISDIFD 

       430        440        450        460        470        480 
GSILSLVQCL TCDRVSTTIE TFQDLSLPIP GKEDLAKLHS TIHQSAVSKA GTCGDSYAAQ 

       490        500        510        520        530        540 
GWLSFFMDYI RRFVVSCIPS WFWGPMITLE DCLAAFFAAD ELKGDNMYSC ERCKKLRNGV 

       550        560        570        580        590        600 
KYCKVLRLPE ILCIHLKRFR HEVMYSFKIG SHVSFPLEGL NLRPFLAKEC VSRITTYDLL 

       610        620        630        640        650        660 
AVICHHGSAS SGHYISYCQN VINGQWYEFD DQYVTEVHET VVQNAEAYVL FYRKSSEEAE 

       670        680        690        700        710        720 
RERQKVVSLA AMKESGLLQF YISREWLNKF NTFAEPGPIS NQSFLCSHGG IPPNKYHYID 

       730        740        750        760        770        780 
DLVVILPQSV WEYLYNRFGG GPAVNHLYVC SICQVEIEAL AKRRKTEIDT FIKLNKAFQA 

       790        800        810        820        830        840 
EEAPSVIYCI SMQWFREWEA FVKAKDSDPP GPIDNSKVAL TKSSGQVQLK QGADYGQISE 

       850        860        870        880 
ETWNYLLNVY GGGPEIAIRQ TVAQYQEAEH LHGEQKIEAE TRAG 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC125749 mRNA. Translation: AAI25750.1.
RefSeqNP_001090641.1. NM_001097172.1.
UniGeneStr.43868.

3D structure databases

ProteinModelPortalA0JM59.
SMRA0JM59. Positions 6-93.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING8364.ENSXETP00000028068.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100036606.
KEGGxtr:100036606.

Organism-specific databases

CTD10868.
XenbaseXB-GENE-966528. usp20.

Phylogenomic databases

eggNOGCOG5560.
HOVERGENHBG054196.
KOK11848.

Family and domain databases

Gene3D3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF06337. DUSP. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMSSF143791. SSF143791. 2 hits.
PROSITEPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP20_XENTR
AccessionPrimary (citable) accession number: A0JM59
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries