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A0JM59

- UBP20_XENTR

UniProt

A0JM59 - UBP20_XENTR

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Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

usp20

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme involved in beta-2 adrenergic receptor (adrb2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (adrb2). Plays a central role in adrb2 recycling and resensitization after prolonged agonist stimulation by constitutively binding adrb2, mediating deubiquitination of adrb2 and inhibiting lysosomal trafficking of adrb2. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei154 – 1541NucleophilePROSITE-ProRule annotation
Active sitei613 – 6131Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: InterPro
  3. ubiquitin thiolesterase activity Source: UniProtKB
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
  2. protein deubiquitination Source: UniProtKB
  3. protein K48-linked deubiquitination Source: UniProtKB
  4. protein K63-linked deubiquitination Source: UniProtKB
  5. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 20
Ubiquitin thioesterase 20
Ubiquitin-specific-processing protease 20
Gene namesi
Name:usp20
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-966528. usp20.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 884884Ubiquitin carboxyl-terminal hydrolase 20PRO_0000390422Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000028068.

Structurei

3D structure databases

ProteinModelPortaliA0JM59.
SMRiA0JM59. Positions 6-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini145 – 655511USPAdd
BLAST
Domaini657 – 75094DUSP 1PROSITE-ProRule annotationAdd
BLAST
Domaini759 – 862104DUSP 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue (By similarity).By similarity

Sequence similaritiesi

Contains 2 DUSP domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
HOVERGENiHBG054196.
InParanoidiA0JM59.
KOiK11848.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0JM59-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGDAEDFCPH LDSIGEVTKE DLILKSKGTC ESCGVGGPNL WACLQDGCQS
60 70 80 90 100
VGCGESYVDH STLHAQAKKH NLTVNLTTFR VWCYACEKEV FLDPRGPPAS
110 120 130 140 150
QTTSPRLSHR DFPTSAHPLK SVPIAVGDDG ESESDEDDIK PRGLTGMKNI
160 170 180 190 200
GNSCYMNAAL QALSNCPPLT QFFLECGGLV RTDKKPALCK SYQKLISELW
210 220 230 240 250
HKKRPSYVVP SSLYHGIKLI NPLFRGYSQQ DTQEFLRCLM DQLHEELKEP
260 270 280 290 300
VPLETQEREE EDRDDQREGE RGGTVEEDFL SCDSGGEMGD GEGGGGVGTL
310 320 330 340 350
SEMELLIREE VGRGLSEKEK LKERKLSYCH RRTSSEQADE DADVDTAMIP
360 370 380 390 400
EPDNDAYVHC SSRSCSPHPV ESISKHSSTP PRSSPLRTSH SYVLKKAQVL
410 420 430 440 450
SGGKKRSEVR YRSVISDIFD GSILSLVQCL TCDRVSTTIE TFQDLSLPIP
460 470 480 490 500
GKEDLAKLHS TIHQSAVSKA GTCGDSYAAQ GWLSFFMDYI RRFVVSCIPS
510 520 530 540 550
WFWGPMITLE DCLAAFFAAD ELKGDNMYSC ERCKKLRNGV KYCKVLRLPE
560 570 580 590 600
ILCIHLKRFR HEVMYSFKIG SHVSFPLEGL NLRPFLAKEC VSRITTYDLL
610 620 630 640 650
AVICHHGSAS SGHYISYCQN VINGQWYEFD DQYVTEVHET VVQNAEAYVL
660 670 680 690 700
FYRKSSEEAE RERQKVVSLA AMKESGLLQF YISREWLNKF NTFAEPGPIS
710 720 730 740 750
NQSFLCSHGG IPPNKYHYID DLVVILPQSV WEYLYNRFGG GPAVNHLYVC
760 770 780 790 800
SICQVEIEAL AKRRKTEIDT FIKLNKAFQA EEAPSVIYCI SMQWFREWEA
810 820 830 840 850
FVKAKDSDPP GPIDNSKVAL TKSSGQVQLK QGADYGQISE ETWNYLLNVY
860 870 880
GGGPEIAIRQ TVAQYQEAEH LHGEQKIEAE TRAG
Length:884
Mass (Da):99,135
Last modified:December 12, 2006 - v1
Checksum:iDA651CCD8694FFF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC125749 mRNA. Translation: AAI25750.1.
RefSeqiNP_001090641.1. NM_001097172.1.
UniGeneiStr.43868.

Genome annotation databases

GeneIDi100036606.
KEGGixtr:100036606.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC125749 mRNA. Translation: AAI25750.1 .
RefSeqi NP_001090641.1. NM_001097172.1.
UniGenei Str.43868.

3D structure databases

ProteinModelPortali A0JM59.
SMRi A0JM59. Positions 6-93.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 8364.ENSXETP00000028068.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100036606.
KEGGi xtr:100036606.

Organism-specific databases

CTDi 10868.
Xenbasei XB-GENE-966528. usp20.

Phylogenomic databases

eggNOGi COG5560.
HOVERGENi HBG054196.
InParanoidi A0JM59.
KOi K11848.

Family and domain databases

Gene3Di 3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF06337. DUSP. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 2 hits.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 2 hits.
PROSITEi PS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiUBP20_XENTR
AccessioniPrimary (citable) accession number: A0JM59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: December 12, 2006
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3