Ubiquitin carboxyl-terminal hydrolase 20 - Xenopus tropicalis (Western clawed frog)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 20
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 20
Ubiquitin thioesterase 20
Ubiquitin-specific-processing protease 20

Gene names

Name:

usp20

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	884 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deubiquitinating enzyme involved in beta-2 adrenergic receptor (adrb2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (adrb2). Plays a central role in adrb2 recycling and resensitization after prolonged agonist stimulation by constitutively binding adrb2, mediating deubiquitination of adrb2 and inhibiting lysosomal trafficking of adrb2. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm › perinuclear region By similarity.
Domain	The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.
Sequence similarities	Belongs to the peptidase C19 family. USP20/USP33 subfamily. Contains 2 DUSP domains. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Endocytosis Ubl conjugation pathway
Cellular component	Cytoplasm
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW protein K48-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB regulation of G-protein coupled receptor protein signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 884

884

Ubiquitin carboxyl-terminal hydrolase 20

PRO_0000390422

Regions

Domain

657 – 750

94

DUSP 1

Domain

759 – 862

104

DUSP 2

Zinc finger

28 – 92

65

UBP-type

Sites

Active site

154

1

Nucleophile By similarity

Active site

613

1

Proton acceptor By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A0JM59 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: DA651CCD8694FFF1
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FASTA

884

99,135

        10         20         30         40         50         60 
MGDAEDFCPH LDSIGEVTKE DLILKSKGTC ESCGVGGPNL WACLQDGCQS VGCGESYVDH 

        70         80         90        100        110        120 
STLHAQAKKH NLTVNLTTFR VWCYACEKEV FLDPRGPPAS QTTSPRLSHR DFPTSAHPLK 

       130        140        150        160        170        180 
SVPIAVGDDG ESESDEDDIK PRGLTGMKNI GNSCYMNAAL QALSNCPPLT QFFLECGGLV 

       190        200        210        220        230        240 
RTDKKPALCK SYQKLISELW HKKRPSYVVP SSLYHGIKLI NPLFRGYSQQ DTQEFLRCLM 

       250        260        270        280        290        300 
DQLHEELKEP VPLETQEREE EDRDDQREGE RGGTVEEDFL SCDSGGEMGD GEGGGGVGTL 

       310        320        330        340        350        360 
SEMELLIREE VGRGLSEKEK LKERKLSYCH RRTSSEQADE DADVDTAMIP EPDNDAYVHC 

       370        380        390        400        410        420 
SSRSCSPHPV ESISKHSSTP PRSSPLRTSH SYVLKKAQVL SGGKKRSEVR YRSVISDIFD 

       430        440        450        460        470        480 
GSILSLVQCL TCDRVSTTIE TFQDLSLPIP GKEDLAKLHS TIHQSAVSKA GTCGDSYAAQ 

       490        500        510        520        530        540 
GWLSFFMDYI RRFVVSCIPS WFWGPMITLE DCLAAFFAAD ELKGDNMYSC ERCKKLRNGV 

       550        560        570        580        590        600 
KYCKVLRLPE ILCIHLKRFR HEVMYSFKIG SHVSFPLEGL NLRPFLAKEC VSRITTYDLL 

       610        620        630        640        650        660 
AVICHHGSAS SGHYISYCQN VINGQWYEFD DQYVTEVHET VVQNAEAYVL FYRKSSEEAE 

       670        680        690        700        710        720 
RERQKVVSLA AMKESGLLQF YISREWLNKF NTFAEPGPIS NQSFLCSHGG IPPNKYHYID 

       730        740        750        760        770        780 
DLVVILPQSV WEYLYNRFGG GPAVNHLYVC SICQVEIEAL AKRRKTEIDT FIKLNKAFQA 

       790        800        810        820        830        840 
EEAPSVIYCI SMQWFREWEA FVKAKDSDPP GPIDNSKVAL TKSSGQVQLK QGADYGQISE 

       850        860        870        880 
ETWNYLLNVY GGGPEIAIRQ TVAQYQEAEH LHGEQKIEAE TRAG

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References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC125749 mRNA. Translation: AAI25750.1.
RefSeq	NP_001090641.1. NM_001097172.1.
UniGene	Str.43868.
3D structure databases
ProteinModelPortal	A0JM59.
SMR	A0JM59. Positions 6-93.
ModBase	Search...
Protein-protein interaction databases
STRING	8364.ENSXETP00000028068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100036606.
KEGG	xtr:100036606.
Organism-specific databases
CTD	10868.
Xenbase	XB-GENE-966528. usp20.
Phylogenomic databases
eggNOG	COG5560.
HOVERGEN	HBG054196.
KO	K11848.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR006615. Pept_C19_DUSP. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF06337. DUSP. 2 hits. PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
SMART	SM00695. DUSP. 2 hits. [Graphical view]
PROSITE	PS51283. DUSP. 2 hits. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP20_XENTR

Accession

Primary (citable) accession number: A0JM59

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2009
Last sequence update:	December 12, 2006
Last modified:	April 3, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents