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A0JLT2

- MED19_HUMAN

UniProt

A0JLT2 - MED19_HUMAN

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Protein

Mediator of RNA polymerase II transcription subunit 19

Gene

MED19

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

  1. RNA polymerase II transcription cofactor activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 19
Alternative name(s):
Lung cancer metastasis-related protein 1
Mediator complex subunit 19
Gene namesi
Name:MED19
Synonyms:LCMR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:29600. MED19.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. mediator complex Source: Ensembl
  2. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134926032.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244Mediator of RNA polymerase II transcription subunit 19PRO_0000304766Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei226 – 2261Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA0JLT2.
PaxDbiA0JLT2.
PRIDEiA0JLT2.

PTM databases

PhosphoSiteiA0JLT2.

Expressioni

Gene expression databases

BgeeiA0JLT2.
CleanExiHS_MED19.
ExpressionAtlasiA0JLT2. baseline and differential.
GenevestigatoriA0JLT2.

Organism-specific databases

HPAiHPA039912.
HPA040860.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.1 Publication

Protein-protein interaction databases

BioGridi128553. 55 interactions.
IntActiA0JLT2. 118 interactions.
MINTiMINT-1370291.
STRINGi9606.ENSP00000337340.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 5340Pro-richAdd
BLAST
Compositional biasi172 – 22251Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 19 family.Curated

Phylogenomic databases

eggNOGiNOG264473.
GeneTreeiENSGT00390000001774.
HOGENOMiHOG000047323.
HOVERGENiHBG055317.
InParanoidiA0JLT2.
KOiK15137.
OrthoDBiEOG7XSTG9.
PhylomeDBiA0JLT2.
TreeFamiTF317417.

Family and domain databases

InterProiIPR019403. Mediator_Med19_met.
[Graphical view]
PfamiPF10278. Med19. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A0JLT2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENFTALFGA QADPPPPPTA LGFGPGKPPP PPPPPAGGGP GTAPPPTAAT
60 70 80 90 100
APPGADKSGA GCGPFYLMRE LPGSTELTGS TNLITHYNLE QAYNKFCGKK
110 120 130 140 150
VKEKLSNFLP DLPGMIDLPG SHDNSSLRSL IEKPPILSSS FNPITGTMLA
160 170 180 190 200
GFRLHTGPLP EQCRLMHIQP PKKKNKHKHK QSRTQDPVPP ETPSDSDHKK
210 220 230 240
KKKKKEEDPD RKRKKKEKKK KKNRHSPDHP GMGSSQASSS SSLR
Length:244
Mass (Da):26,273
Last modified:October 2, 2007 - v2
Checksum:i9B85721364F33F02
GO
Isoform 2 (identifier: A0JLT2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     191-194: ETPS → GKPS
     195-244: Missing.

Note: No experimental confirmation available.

Show »
Length:194
Mass (Da):20,431
Checksum:i38F9038E0795835E
GO

Sequence cautioni

The sequence AAN16075.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981H → P in AAN16075. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei191 – 1944ETPS → GKPS in isoform 2. 1 PublicationVSP_028121
Alternative sequencei195 – 24450Missing in isoform 2. 1 PublicationVSP_028122Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH471076 Genomic DNA. Translation: EAW73775.1.
BC009723 mRNA. Translation: AAH09723.1.
BC037223 mRNA. Translation: AAH37223.1.
AY148462 mRNA. Translation: AAN16075.1. Different initiation.
CCDSiCCDS7966.1. [A0JLT2-2]
RefSeqiNP_703151.1. NM_153450.1. [A0JLT2-2]
UniGeneiHs.43619.

Genome annotation databases

EnsembliENST00000337672; ENSP00000337340; ENSG00000156603. [A0JLT2-2]
GeneIDi219541.
KEGGihsa:219541.
UCSCiuc001nlb.3. human. [A0JLT2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH471076 Genomic DNA. Translation: EAW73775.1 .
BC009723 mRNA. Translation: AAH09723.1 .
BC037223 mRNA. Translation: AAH37223.1 .
AY148462 mRNA. Translation: AAN16075.1 . Different initiation.
CCDSi CCDS7966.1. [A0JLT2-2 ]
RefSeqi NP_703151.1. NM_153450.1. [A0JLT2-2 ]
UniGenei Hs.43619.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 128553. 55 interactions.
IntActi A0JLT2. 118 interactions.
MINTi MINT-1370291.
STRINGi 9606.ENSP00000337340.

PTM databases

PhosphoSitei A0JLT2.

Proteomic databases

MaxQBi A0JLT2.
PaxDbi A0JLT2.
PRIDEi A0JLT2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000337672 ; ENSP00000337340 ; ENSG00000156603 . [A0JLT2-2 ]
GeneIDi 219541.
KEGGi hsa:219541.
UCSCi uc001nlb.3. human. [A0JLT2-2 ]

Organism-specific databases

CTDi 219541.
GeneCardsi GC11M057471.
H-InvDB HIX0026171.
HGNCi HGNC:29600. MED19.
HPAi HPA039912.
HPA040860.
MIMi 612385. gene.
neXtProti NX_A0JLT2.
PharmGKBi PA134926032.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG264473.
GeneTreei ENSGT00390000001774.
HOGENOMi HOG000047323.
HOVERGENi HBG055317.
InParanoidi A0JLT2.
KOi K15137.
OrthoDBi EOG7XSTG9.
PhylomeDBi A0JLT2.
TreeFami TF317417.

Enzyme and pathway databases

Reactomei REACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

GenomeRNAii 219541.
NextBioi 90685.
PROi A0JLT2.
SOURCEi Search...

Gene expression databases

Bgeei A0JLT2.
CleanExi HS_MED19.
ExpressionAtlasi A0JLT2. baseline and differential.
Genevestigatori A0JLT2.

Family and domain databases

InterProi IPR019403. Mediator_Med19_met.
[Graphical view ]
Pfami PF10278. Med19. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-205 (ISOFORM 1).
    Tissue: Brain and Lung.
  3. "Cloning of a metastasis-related gene cDNA from a human lung cancer cell line."
    Chen L.A., Tian Q., Liu Y.N., Fan B.X.
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-244 (ISOFORM 1).
  4. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
    Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
    J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED10 AND MED31.
  5. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiMED19_HUMAN
AccessioniPrimary (citable) accession number: A0JLT2
Secondary accession number(s): Q8IV02, Q8IZD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: October 29, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3