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A0JLT2

- MED19_HUMAN

UniProt

A0JLT2 - MED19_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 19

Gene

MED19

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

    GO - Molecular functioni

    1. RNA polymerase II transcription cofactor activity Source: InterPro

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 19
    Alternative name(s):
    Lung cancer metastasis-related protein 1
    Mediator complex subunit 19
    Gene namesi
    Name:MED19
    Synonyms:LCMR1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:29600. MED19.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: Ensembl
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134926032.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 244244Mediator of RNA polymerase II transcription subunit 19PRO_0000304766Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei226 – 2261Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiA0JLT2.
    PaxDbiA0JLT2.
    PRIDEiA0JLT2.

    PTM databases

    PhosphoSiteiA0JLT2.

    Expressioni

    Gene expression databases

    ArrayExpressiA0JLT2.
    BgeeiA0JLT2.
    CleanExiHS_MED19.
    GenevestigatoriA0JLT2.

    Organism-specific databases

    HPAiHPA039912.
    HPA040860.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.1 Publication

    Protein-protein interaction databases

    BioGridi128553. 53 interactions.
    IntActiA0JLT2. 118 interactions.
    MINTiMINT-1370291.
    STRINGi9606.ENSP00000337340.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi14 – 5340Pro-richAdd
    BLAST
    Compositional biasi172 – 22251Lys-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 19 family.Curated

    Phylogenomic databases

    eggNOGiNOG264473.
    HOGENOMiHOG000047323.
    HOVERGENiHBG055317.
    InParanoidiA0JLT2.
    KOiK15137.
    OrthoDBiEOG7XSTG9.
    PhylomeDBiA0JLT2.
    TreeFamiTF317417.

    Family and domain databases

    InterProiIPR019403. Mediator_Med19_met.
    [Graphical view]
    PfamiPF10278. Med19. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A0JLT2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MENFTALFGA QADPPPPPTA LGFGPGKPPP PPPPPAGGGP GTAPPPTAAT    50
    APPGADKSGA GCGPFYLMRE LPGSTELTGS TNLITHYNLE QAYNKFCGKK 100
    VKEKLSNFLP DLPGMIDLPG SHDNSSLRSL IEKPPILSSS FNPITGTMLA 150
    GFRLHTGPLP EQCRLMHIQP PKKKNKHKHK QSRTQDPVPP ETPSDSDHKK 200
    KKKKKEEDPD RKRKKKEKKK KKNRHSPDHP GMGSSQASSS SSLR 244
    Length:244
    Mass (Da):26,273
    Last modified:October 2, 2007 - v2
    Checksum:i9B85721364F33F02
    GO
    Isoform 2 (identifier: A0JLT2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         191-194: ETPS → GKPS
         195-244: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:194
    Mass (Da):20,431
    Checksum:i38F9038E0795835E
    GO

    Sequence cautioni

    The sequence AAN16075.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti198 – 1981H → P in AAN16075. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei191 – 1944ETPS → GKPS in isoform 2. 1 PublicationVSP_028121
    Alternative sequencei195 – 24450Missing in isoform 2. 1 PublicationVSP_028122Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH471076 Genomic DNA. Translation: EAW73775.1.
    BC009723 mRNA. Translation: AAH09723.1.
    BC037223 mRNA. Translation: AAH37223.1.
    AY148462 mRNA. Translation: AAN16075.1. Different initiation.
    CCDSiCCDS7966.1. [A0JLT2-2]
    RefSeqiNP_703151.1. NM_153450.1. [A0JLT2-2]
    UniGeneiHs.43619.

    Genome annotation databases

    EnsembliENST00000337672; ENSP00000337340; ENSG00000156603. [A0JLT2-2]
    GeneIDi219541.
    KEGGihsa:219541.
    UCSCiuc001nlb.3. human. [A0JLT2-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH471076 Genomic DNA. Translation: EAW73775.1 .
    BC009723 mRNA. Translation: AAH09723.1 .
    BC037223 mRNA. Translation: AAH37223.1 .
    AY148462 mRNA. Translation: AAN16075.1 . Different initiation.
    CCDSi CCDS7966.1. [A0JLT2-2 ]
    RefSeqi NP_703151.1. NM_153450.1. [A0JLT2-2 ]
    UniGenei Hs.43619.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 128553. 53 interactions.
    IntActi A0JLT2. 118 interactions.
    MINTi MINT-1370291.
    STRINGi 9606.ENSP00000337340.

    PTM databases

    PhosphoSitei A0JLT2.

    Proteomic databases

    MaxQBi A0JLT2.
    PaxDbi A0JLT2.
    PRIDEi A0JLT2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000337672 ; ENSP00000337340 ; ENSG00000156603 . [A0JLT2-2 ]
    GeneIDi 219541.
    KEGGi hsa:219541.
    UCSCi uc001nlb.3. human. [A0JLT2-2 ]

    Organism-specific databases

    CTDi 219541.
    GeneCardsi GC11M057471.
    H-InvDB HIX0026171.
    HGNCi HGNC:29600. MED19.
    HPAi HPA039912.
    HPA040860.
    MIMi 612385. gene.
    neXtProti NX_A0JLT2.
    PharmGKBi PA134926032.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG264473.
    HOGENOMi HOG000047323.
    HOVERGENi HBG055317.
    InParanoidi A0JLT2.
    KOi K15137.
    OrthoDBi EOG7XSTG9.
    PhylomeDBi A0JLT2.
    TreeFami TF317417.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    GenomeRNAii 219541.
    NextBioi 90685.
    PROi A0JLT2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi A0JLT2.
    Bgeei A0JLT2.
    CleanExi HS_MED19.
    Genevestigatori A0JLT2.

    Family and domain databases

    InterProi IPR019403. Mediator_Med19_met.
    [Graphical view ]
    Pfami PF10278. Med19. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-205 (ISOFORM 1).
      Tissue: Brain and Lung.
    3. "Cloning of a metastasis-related gene cDNA from a human lung cancer cell line."
      Chen L.A., Tian Q., Liu Y.N., Fan B.X.
      Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-244 (ISOFORM 1).
    4. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
      Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
      J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED10 AND MED31.
    5. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiMED19_HUMAN
    AccessioniPrimary (citable) accession number: A0JLT2
    Secondary accession number(s): Q8IV02, Q8IZD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3