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Protein

Extended synaptotagmin-3

Gene

ESYT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Tethers the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi321Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi322Calcium 1By similarity1
Metal bindingi322Calcium 2By similarity1
Metal bindingi332Calcium 2By similarity1
Metal bindingi379Calcium 1By similarity1
Metal bindingi379Calcium 2By similarity1
Metal bindingi380Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi381Calcium 1By similarity1
Metal bindingi381Calcium 2By similarity1
Metal bindingi381Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi383Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi385Calcium 3By similarity1
Metal bindingi386Calcium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Calcium, Lipid-binding, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1660662. Glycosphingolipid metabolism.

Protein family/group databases

TCDBi9.A.57.1.3. the extended-synaptotagmin (e-syt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Extended synaptotagmin-3
Short name:
E-Syt3
Alternative name(s):
Chr3Syt
Gene namesi
Name:ESYT3
Synonyms:FAM62C
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:24295. ESYT3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicSequence analysisAdd BLAST29
Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
Topological domaini72 – 886CytoplasmicSequence analysisAdd BLAST815

GO - Cellular componenti

  • extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  • integral component of plasma membrane Source: FlyBase
  • intrinsic component of endoplasmic reticulum membrane Source: UniProtKB
  • organelle membrane contact site Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi83850.
OpenTargetsiENSG00000158220.
PharmGKBiPA165697118.

Polymorphism and mutation databases

BioMutaiESYT3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003148991 – 886Extended synaptotagmin-3Add BLAST886

Proteomic databases

PaxDbiA0FGR9.
PRIDEiA0FGR9.

PTM databases

iPTMnetiA0FGR9.
PhosphoSitePlusiA0FGR9.

Expressioni

Tissue specificityi

Widely expressed with high level in cerebellum and skin.1 Publication

Gene expression databases

BgeeiENSG00000158220.
CleanExiHS_FAM62C.
ExpressionAtlasiA0FGR9. baseline and differential.
GenevisibleiA0FGR9. HS.

Organism-specific databases

HPAiHPA039200.

Interactioni

Subunit structurei

Interacts with ESYT1 and ESYT2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ESYT1Q9BSJ83EBI-8771391,EBI-355956
ESYT2A0FGR83EBI-8771391,EBI-3184170

Protein-protein interaction databases

BioGridi123766. 1 interactor.
DIPiDIP-61998N.
IntActiA0FGR9. 2 interactors.
STRINGi9606.ENSP00000374218.

Structurei

3D structure databases

ProteinModelPortaliA0FGR9.
SMRiA0FGR9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini293 – 392C2 1PROSITE-ProRule annotationAdd BLAST100
Domaini430 – 544C2 2PROSITE-ProRule annotationAdd BLAST115
Domaini756 – 860C2 3PROSITE-ProRule annotationAdd BLAST105

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 291Glycerophospholipid-binding barrel-like domainBy similarityAdd BLAST174
Regioni801 – 808Required for phosphatidylinositol 4,5-bisphosphate-dependent location at the cell membraneBy similarity8

Domaini

Anchored to the endoplasmic reticulum membrane by a transmembrane hairpin structure; both N-terminus and C-terminus are cytoplasmic.1 Publication
The C2 domains mediate lipid and calcium binding. The N-terminal C2 domain binds calcium ions and is important for calcium-dependent lipid binding and interaction with membranes. Two calcium ions are bound at a high-affinity site and a third calcium ion is bound with lower affinity. May bind up to four calcium ions. In contrast, the second C2 domain apparently does not bind calcium (By similarity). The third C2 domain mediates interaction with membranes enriched in phosphatidylinositol 4,5-bisphosphate and is required for location at the cell membrane (PubMed:23791178).By similarity1 Publication
Contains a barrel-like domain that can bind various types of glycerophospholipids in its interior.By similarity

Sequence similaritiesi

Belongs to the extended synaptotagmin family.Curated
Contains 3 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1012. Eukaryota.
ENOG410YCXU. LUCA.
GeneTreeiENSGT00550000074417.
HOGENOMiHOG000043080.
HOVERGENiHBG055795.
InParanoidiA0FGR9.
OMAiAWPPKRL.
OrthoDBiEOG091G02X7.
PhylomeDBiA0FGR9.
TreeFamiTF324255.

Family and domain databases

Gene3Di2.60.40.150. 3 hits.
InterProiIPR000008. C2_dom.
IPR031468. SMP_LBD.
[Graphical view]
PfamiPF00168. C2. 3 hits.
PF17047. SMP_LBD. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00239. C2. 3 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 3 hits.
PROSITEiPS50004. C2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A0FGR9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRAEEPCAPG APSALGAQRT PGPELRLSSQ LLPELCTFVV RVLFYLGPVY
60 70 80 90 100
LAGYLGLSIT WLLLGALLWM WWRRNRRGKL GRLAAAFEFL DNEREFISRE
110 120 130 140 150
LRGQHLPAWI HFPDVERVEW ANKIISQTWP YLSMIMESKF REKLEPKIRE
160 170 180 190 200
KSIHLRTFTF TKLYFGQKCP RVNGVKAHTN TCNRRRVTVD LQICYIGDCE
210 220 230 240 250
ISVELQKIQA GVNGIQLQGT LRVILEPLLV DKPFVGAVTV FFLQKPHLQI
260 270 280 290 300
NWTGLTNLLD APGINDVSDS LLEDLIATHL VLPNRVTVPV KKGLDLTNLR
310 320 330 340 350
FPLPCGVIRV HLLEAEQLAQ KDNFLGLRGK SDPYAKVSIG LQHFRSRTIY
360 370 380 390 400
RNLNPTWNEV FEFMVYEVPG QDLEVDLYDE DTDRDDFLGS LQICLGDVMT
410 420 430 440 450
NRVVDEWFVL NDTTSGRLHL RLEWLSLLTD QEVLTEDHGG LSTAILVVFL
460 470 480 490 500
ESACNLPRNP FDYLNGEYRA KKLSRFARNK VSKDPSSYVK LSVGKKTHTS
510 520 530 540 550
KTCPHNKDPV WSQVFSFFVH NVATERLHLK VLDDDQECAL GMLEVPLCQI
560 570 580 590 600
LPYADLTLEQ RFQLDHSGLD SLISMRLVLR FLQVEERELG SPYTGPEALK
610 620 630 640 650
KGPLLIKKVA TNQGPKAQPQ EEGPTDLPCP PDPASDTKDV SRSTTTTTSA
660 670 680 690 700
TTVATEPTSQ ETGPEPKGKD SAKRFCEPIG EKKSPATIFL TVPGPHSPGP
710 720 730 740 750
IKSPRPMKCP ASPFAWPPKR LAPSMSSLNS LASSCFDLAD ISLNIEGGDL
760 770 780 790 800
RRRQLGEIQL TVRYVCLRRC LSVLINGCRN LTPCTSSGAD PYVRVYLLPE
810 820 830 840 850
RKWACRKKTS VKRKTLEPLF DETFEFFVPM EEVKKRSLDV AVKNSRPLGS
860 870 880
HRRKELGKVL IDLSKEDLIK GFSQWYELTP NGQPRS
Length:886
Mass (Da):100,035
Last modified:November 28, 2006 - v1
Checksum:i5CFE68F790D11B9B
GO
Isoform 2 (identifier: A0FGR9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     479-501: NKVSKDPSSYVKLSVGKKTHTSK → VKQGQQRPFFLCQTICRQEDTYK
     502-886: Missing.

Show »
Length:501
Mass (Da):57,502
Checksum:i4B0951E0B2D5DDF1
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_038117246P → Q.1 PublicationCorresponds to variant rs17857138dbSNPEnsembl.1
Natural variantiVAR_038118416G → R.Corresponds to variant rs6772467dbSNPEnsembl.1
Natural variantiVAR_053835590G → R.Corresponds to variant rs10935282dbSNPEnsembl.1
Natural variantiVAR_062173662T → S.Corresponds to variant rs35537868dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030424479 – 501NKVSK…THTSK → VKQGQQRPFFLCQTICRQED TYK in isoform 2. 2 PublicationsAdd BLAST23
Alternative sequenceiVSP_030425502 – 886Missing in isoform 2. 2 PublicationsAdd BLAST385

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ993202 mRNA. Translation: ABJ97707.1.
AK289530 mRNA. Translation: BAF82219.1.
BC037292 mRNA. Translation: AAH37292.1.
AJ303366 mRNA. Translation: CAC33888.1.
CCDSiCCDS3101.2. [A0FGR9-1]
RefSeqiNP_001309760.1. NM_001322831.1. [A0FGR9-1]
NP_114119.2. NM_031913.4. [A0FGR9-1]
UniGeneiHs.477711.

Genome annotation databases

EnsembliENST00000389567; ENSP00000374218; ENSG00000158220. [A0FGR9-1]
ENST00000490835; ENSP00000417388; ENSG00000158220. [A0FGR9-2]
GeneIDi83850.
KEGGihsa:83850.
UCSCiuc003esk.4. human. [A0FGR9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ993202 mRNA. Translation: ABJ97707.1.
AK289530 mRNA. Translation: BAF82219.1.
BC037292 mRNA. Translation: AAH37292.1.
AJ303366 mRNA. Translation: CAC33888.1.
CCDSiCCDS3101.2. [A0FGR9-1]
RefSeqiNP_001309760.1. NM_001322831.1. [A0FGR9-1]
NP_114119.2. NM_031913.4. [A0FGR9-1]
UniGeneiHs.477711.

3D structure databases

ProteinModelPortaliA0FGR9.
SMRiA0FGR9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123766. 1 interactor.
DIPiDIP-61998N.
IntActiA0FGR9. 2 interactors.
STRINGi9606.ENSP00000374218.

Protein family/group databases

TCDBi9.A.57.1.3. the extended-synaptotagmin (e-syt) family.

PTM databases

iPTMnetiA0FGR9.
PhosphoSitePlusiA0FGR9.

Polymorphism and mutation databases

BioMutaiESYT3.

Proteomic databases

PaxDbiA0FGR9.
PRIDEiA0FGR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000389567; ENSP00000374218; ENSG00000158220. [A0FGR9-1]
ENST00000490835; ENSP00000417388; ENSG00000158220. [A0FGR9-2]
GeneIDi83850.
KEGGihsa:83850.
UCSCiuc003esk.4. human. [A0FGR9-1]

Organism-specific databases

CTDi83850.
DisGeNETi83850.
GeneCardsiESYT3.
HGNCiHGNC:24295. ESYT3.
HPAiHPA039200.
neXtProtiNX_A0FGR9.
OpenTargetsiENSG00000158220.
PharmGKBiPA165697118.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1012. Eukaryota.
ENOG410YCXU. LUCA.
GeneTreeiENSGT00550000074417.
HOGENOMiHOG000043080.
HOVERGENiHBG055795.
InParanoidiA0FGR9.
OMAiAWPPKRL.
OrthoDBiEOG091G02X7.
PhylomeDBiA0FGR9.
TreeFamiTF324255.

Enzyme and pathway databases

ReactomeiR-HSA-1660662. Glycosphingolipid metabolism.

Miscellaneous databases

GenomeRNAii83850.
PROiA0FGR9.

Gene expression databases

BgeeiENSG00000158220.
CleanExiHS_FAM62C.
ExpressionAtlasiA0FGR9. baseline and differential.
GenevisibleiA0FGR9. HS.

Family and domain databases

Gene3Di2.60.40.150. 3 hits.
InterProiIPR000008. C2_dom.
IPR031468. SMP_LBD.
[Graphical view]
PfamiPF00168. C2. 3 hits.
PF17047. SMP_LBD. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00239. C2. 3 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 3 hits.
PROSITEiPS50004. C2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiESYT3_HUMAN
AccessioniPrimary (citable) accession number: A0FGR9
Secondary accession number(s): A8K0G5
, Q6ZV21, Q8NDZ5, Q9BQR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 28, 2006
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.