A0B9U4 (A0B9U4_METTP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Probable tRNA pseudouridine synthase B HAMAP-Rule MF_01081
EC=5.4.99.25 HAMAP-Rule MF_01081

Alternative name(s):
tRNA pseudouridine(55) synthase HAMAP-Rule MF_01081
tRNA pseudouridylate synthase HAMAP-Rule MF_01081
tRNA-uridine isomerase HAMAP-Rule MF_01081

Gene names

Name:	truB HAMAP-Rule MF_01081
Ordered Locus Names:	Mthe_1702

Organism

Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP] EMBL ABK15468.1

Taxonomic identifier

349307 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosaetaceae › Methanosaeta › Methanosaeta thermophila

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01081
Catalytic activity	tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01081
Sequence similarities	Belongs to the pseudouridine synthase TruB family. Type 2 subfamily. HAMAP-Rule MF_01081 Contains 1 PUA domain. HAMAP-Rule MF_01081

Ontologies

Keywords
Biological process	tRNA processing HAMAP-Rule MF_01081
Molecular function	Isomerase HAMAP-Rule MF_01081 Lyase EMBL ABK15468.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro lyase activity Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	230 – 305	76	PUA By similarity HAMAP-Rule MF_01081
Sites
	Active site	62	1	Nucleophile By similarity HAMAP-Rule MF_01081

Sequences

	Sequence		Length	Mass (Da)
	A0B9U4 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: D15477BBEAC78DD5	FASTA	321	35,520
	10 20 30 40 50 60 MRREGSTNSS YGCFPDRRPL QEHLRLGAIN LDKPSGPTSH EVVAWVKRIL GLEKAGHSGT 70 80 90 100 110 120 LDPRVTGILP VLTGDATRVV EFLLTAGKEY VCLMRTHEQV PRKKILEVCE EFSGEIYQRP 130 140 150 160 170 180 PLKSSVARNL RTRKIYYIDV LEIEGQRVLM KVGCEAGTYI RKLCYDMGLA LGCGANMEEL 190 200 210 220 230 240 RRTKAGPFAE DETLVTLHNL KDAYEIWRES GDESALRRAV LPVERALRHL PGLVISDNAV 250 260 270 280 290 300 DAICHGAPLA APGLLSLETD INKGDFVVIY TLKGEAVAIG RAKLSSDEMM VAKSGIVAST 310 320 ERVIMDPGVY PRRWKHSAEV V

References

[1]

"Complete sequence of Methanosaeta thermophila PT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Smith K.S., Ingram-Smith C., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6194 / PT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000477 Genomic DNA. Translation: ABK15468.1.
RefSeq	YP_844108.1. NC_008553.1.
3D structure databases
ProteinModelPortal	A0B9U4.
SMR	A0B9U4. Positions 4-314.
ModBase	Search...
Protein-protein interaction databases
STRING	349307.Mthe_1702.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK15468; ABK15468; Mthe_1702.
GeneID	4462662.
KEGG	mtp:Mthe_1702.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0130.
HOGENOM	HOG000231224.
KO	K11131.
OMA	VDAICHG.
Enzyme and pathway databases
BioCyc	MMET349307:GHKI-1751-MONOMER.
Family and domain databases
HAMAP	MF_01081. TruB_arch.
InterPro	IPR012960. Dyskerin-like. IPR002501. PsdUridine_synth. IPR020103. PsdUridine_synth_cat_dom. IPR002478. PUA. IPR015947. PUA-like_domain. IPR004802. tRNA_PsdUridine_synth_B_fam. IPR026326. TruB_arch. IPR004521. Uncharacterised_CHP00451. [Graphical view]
PANTHER	PTHR23127. PTHR23127. 1 hit.
Pfam	PF08068. DKCLD. 1 hit. PF01472. PUA. 1 hit. PF01509. TruB_N. 2 hits. [Graphical view]
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR00425. CBF5. 1 hit. TIGR00451. unchar_dom_2. 1 hit.
PROSITE	PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A0B9U4_METTP

Accession

Primary (citable) accession number: A0B9U4

Entry history

Integrated into UniProtKB/TrEMBL:	November 28, 2006
Last sequence update:	November 28, 2006
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)