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Protein

Probable tRNA pseudouridine synthase B

Gene

truB

Organism
Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei62 – 621NucleophileUniRule annotation

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. pseudouridine synthase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: InterPro

GO - Biological processi

  1. RNA processing Source: InterPro
  2. tRNA pseudouridine synthesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotation, LyaseImported

Keywords - Biological processi

tRNA processingUniRule annotation

Enzyme and pathway databases

BioCyciMMET349307:GHKI-1753-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:Mthe_1702Imported
OrganismiMethanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))Imported
Taxonomic identifieri349307 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta
ProteomesiUP000000674 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi349307.Mthe_1702.

Structurei

3D structure databases

ProteinModelPortaliA0B9U4.
SMRiA0B9U4. Positions 4-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini230 – 30576PUAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0130.
HOGENOMiHOG000231224.
KOiK11131.
OMAiPKEYVCL.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01081. TruB_arch.
InterProiIPR012960. Dyskerin-like.
IPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF01509. TruB_N. 2 hits.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0B9U4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRREGSTNSS YGCFPDRRPL QEHLRLGAIN LDKPSGPTSH EVVAWVKRIL
60 70 80 90 100
GLEKAGHSGT LDPRVTGILP VLTGDATRVV EFLLTAGKEY VCLMRTHEQV
110 120 130 140 150
PRKKILEVCE EFSGEIYQRP PLKSSVARNL RTRKIYYIDV LEIEGQRVLM
160 170 180 190 200
KVGCEAGTYI RKLCYDMGLA LGCGANMEEL RRTKAGPFAE DETLVTLHNL
210 220 230 240 250
KDAYEIWRES GDESALRRAV LPVERALRHL PGLVISDNAV DAICHGAPLA
260 270 280 290 300
APGLLSLETD INKGDFVVIY TLKGEAVAIG RAKLSSDEMM VAKSGIVAST
310 320
ERVIMDPGVY PRRWKHSAEV V
Length:321
Mass (Da):35,520
Last modified:November 27, 2006 - v1
Checksum:iD15477BBEAC78DD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA. Translation: ABK15468.1.
RefSeqiYP_844108.1. NC_008553.1.

Genome annotation databases

EnsemblBacteriaiABK15468; ABK15468; Mthe_1702.
GeneIDi4462662.
KEGGimtp:Mthe_1702.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA. Translation: ABK15468.1.
RefSeqiYP_844108.1. NC_008553.1.

3D structure databases

ProteinModelPortaliA0B9U4.
SMRiA0B9U4. Positions 4-314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349307.Mthe_1702.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK15468; ABK15468; Mthe_1702.
GeneIDi4462662.
KEGGimtp:Mthe_1702.

Phylogenomic databases

eggNOGiCOG0130.
HOGENOMiHOG000231224.
KOiK11131.
OMAiPKEYVCL.

Enzyme and pathway databases

BioCyciMMET349307:GHKI-1753-MONOMER.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01081. TruB_arch.
InterProiIPR012960. Dyskerin-like.
IPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF01509. TruB_N. 2 hits.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 6194 / PTImported.

Entry informationi

Entry nameiA0B9U4_METTP
AccessioniPrimary (citable) accession number: A0B9U4
Entry historyi
Integrated into UniProtKB/TrEMBL: November 27, 2006
Last sequence update: November 27, 2006
Last modified: March 31, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.