A0B9N3 (SYP_METTP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Mthe_1640

Organism

Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]

Taxonomic identifier

349307 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosaetaceae › Methanosaeta

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Proline--tRNA ligase HAMAP MF_01571		PRO_0000288422

Sequences

	Sequence		Length	Mass (Da)
	A0B9N3 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 69A9EB7606BB8F77	FASTA	478	54,861
	10 20 30 40 50 60 MTKKEERDAK LPPKSNFSEW YHELLRSAEI VDVRYPVKGM SVWYPFGFAL RSHVYQIIKE 70 80 90 100 110 120 LLDVDHYETQ FPLLIPETEF MKEAEHIKGF EDEVYWVTHG GRDPLDIKLA LRPTSETAIY 130 140 150 160 170 180 PMLKLWIRSH ADLPLRIYQI VNTFRYETKH TRPLIRLREI TSFKEAHTAH ATWEEAAMQV 190 200 210 220 230 240 EIAIQRYIEF YRRLAIPCLV SKRPSWDKFP GADYSIALDV IMPDGRTLQV GTAHLLGTNF 250 260 270 280 290 300 AKTYEITYED EHGEQRYVNQ TCYGISERCI AALIAVHGDD KGLVLPWRVA PVQVVIVPII 310 320 330 340 350 360 FGEREPIIEV CRSIASTLAG RNIRVRLDDG DERPGAKFYK WEMRGVPVRI EIGPRDIKNG 370 380 390 400 410 420 VVTIVRRDGV RKTLPMDERL VDAILIEAEE LQTVLYNRAK EFMDSKIKLV SSLDEARSQV 430 440 450 460 470 QSGVARVPWC GSVECGHALE DQIGANLLGE PRGDELPPMR CLVCGRESTG STYMARQY

References

[1]

"Complete sequence of Methanosaeta thermophila PT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Smith K.S., Ingram-Smith C., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6194 / PT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000477 Genomic DNA. Translation: ABK15407.1.
RefSeq	YP_844047.1. NC_008553.1.
3D structure databases
ProteinModelPortal	A0B9N3.
SMR	A0B9N3. Positions 16-478.
ModBase	Search...
Protein-protein interaction databases
STRING	A0B9N3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4462512.
GenomeReviews	Gene locus Mthe_1640 in contig CP000477_GR.
KEGG	mtp:Mthe_1640.
NMPDR	fig|349307.7.peg.1708.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	KFAEYEL.
PhylomeDB	A0B9N3.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	MTHE349307:MTHE_1640-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_METTP

Accession

Primary (citable) accession number: A0B9N3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	November 28, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program