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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Methanosaeta thermophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT) (Methanothrix thermophila)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Mthe_1466
OrganismiMethanosaeta thermophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT) (Methanothrix thermophila)
Taxonomic identifieri349307 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta
Proteomesi
  • UP000000674 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003310101 – 564Glutamate--tRNA ligaseAdd BLAST564

Interactioni

Protein-protein interaction databases

STRINGi349307.Mthe_1466.

Structurei

3D structure databases

ProteinModelPortaliA0B966.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi107 – 117"HIGH" regionAdd BLAST11

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04302. Archaea.
COG0008. LUCA.
HOGENOMiHOG000073585.
KOiK01885.
OMAiKMYRLME.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A. 1 hit.
InterProiIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

A0B966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEEIRELIE KFALQNAVKY RSAPNHGAVM GKLMGERPDL RPRAREIAPL
60 70 80 90 100
ITSVLNEIER MSPEQWESRL REMAPELIEE ISARKEPAKG LQPLEGAEGG
110 120 130 140 150
VVMRFAPNPN GPPTLGSARG IIINSEYVKM YGGKFILRFD DTDPVNKRPM
160 170 180 190 200
IEAYDWYIED CKWLGAEPHE IVAASQRVEK YYEVAEELIR RGGAYVCLCE
210 220 230 240 250
QTAFKALKDA AMPCPHRDQT VDENMALWNK MLDGSFQEGE AVLRVKTDIK
260 270 280 290 300
HKDPAMRDWA GFRIVTKSHP LVGERYRVWP LLDFESAVED HLLGVTHILR
310 320 330 340 350
GKDLIDSERR QRYLYDHMGW VYPRVLHWGR VKIFQFGAFS TSKLRKAIEA
360 370 380 390 400
GEYSGWDDPR LPTVRAMRRR GITAEALRKF MIDLGVGETD ISISMDSIYA
410 420 430 440 450
ENRRIVDPIA NRRFFVWDPV EIEIKGEVPE VAEAPLHPTI DRGTRRIRAG
460 470 480 490 500
GRLLLCKEDV AGLAPGSRIR LKDLCNIEIK EIFPLRAELI DLSPETAKRL
510 520 530 540 550
KLRIIHWAPV EGIPVRVLGP DKTDTGIGEH GISDELDRIV QFERYGFVRI
560
DSVGEEVVAY FAHR
Length:564
Mass (Da):64,383
Last modified:November 28, 2006 - v1
Checksum:i1D3BE679CE0D901F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA. Translation: ABK15240.1.

Genome annotation databases

EnsemblBacteriaiABK15240; ABK15240; Mthe_1466.
KEGGimtp:Mthe_1466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA. Translation: ABK15240.1.

3D structure databases

ProteinModelPortaliA0B966.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349307.Mthe_1466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK15240; ABK15240; Mthe_1466.
KEGGimtp:Mthe_1466.

Phylogenomic databases

eggNOGiarCOG04302. Archaea.
COG0008. LUCA.
HOGENOMiHOG000073585.
KOiK01885.
OMAiKMYRLME.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A. 1 hit.
InterProiIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_METTP
AccessioniPrimary (citable) accession number: A0B966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 28, 2006
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.