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Protein

Fructose-1,6-bisphosphate aldolase/phosphatase

Gene

fbp

Organism
Methanosaeta thermophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT) (Methanothrix thermophila)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).UniRule annotation

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.UniRule annotation
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei12Proton acceptor; for FBP phosphatase activityUniRule annotation1
Metal bindingi12Magnesium 1UniRule annotation1
Metal bindingi19Magnesium 1; via pros nitrogenUniRule annotation1
Binding sitei19FBP; via tele nitrogenUniRule annotation1
Metal bindingi53Magnesium 1UniRule annotation1
Metal bindingi53Magnesium 2UniRule annotation1
Metal bindingi54Magnesium 2UniRule annotation1
Binding sitei91FBPUniRule annotation1
Metal bindingi95Magnesium 1UniRule annotation1
Metal bindingi132Magnesium 2UniRule annotation1
Binding sitei133FBP/DHAPUniRule annotation1
Active sitei234Proton donor/acceptor; for FBP aldolase activityUniRule annotation1
Active sitei237Schiff-base intermediate with DHAP; for FBP aldolase activityUniRule annotation1
Metal bindingi237Magnesium 3; via carbonyl oxygenUniRule annotation1
Metal bindingi238Magnesium 3UniRule annotation1
Metal bindingi238Magnesium 4UniRule annotation1
Metal bindingi239Magnesium 2UniRule annotation1
Metal bindingi239Magnesium 3UniRule annotation1
Binding sitei271FBP/DHAP; via amide nitrogenUniRule annotation1
Binding sitei292FBP/DHAPUniRule annotation1
Binding sitei353FBPUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolaseUniRule annotation, LyaseUniRule annotationImported
Biological processCarbohydrate metabolismUniRule annotation, GluconeogenesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation, Schiff baseUniRule annotation

Enzyme and pathway databases

BioCyciMTHE349307:G12X8-1343-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphate aldolase/phosphataseUniRule annotation (EC:3.1.3.11UniRule annotation, EC:4.1.2.13UniRule annotation)
Short name:
FBP A/PUniRule annotation
Short name:
FBP aldolase/phosphataseUniRule annotation
Gene namesi
Name:fbpUniRule annotation
Ordered Locus Names:Mthe_1333Imported
OrganismiMethanosaeta thermophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT) (Methanothrix thermophila)Imported
Taxonomic identifieri349307 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta
Proteomesi
  • UP000000674 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; dimer of tetramers.UniRule annotation

Protein-protein interaction databases

STRINGi349307.Mthe_1333.

Structurei

3D structure databases

ProteinModelPortaliA0B8T5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 105FBP bindingUniRule annotation2
Regioni247 – 248FBP binding; shared with dimeric partnerUniRule annotation2

Domaini

Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.UniRule annotation

Sequence similaritiesi

Belongs to the FBP aldolase/phosphatase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04180. Archaea.
COG1980. LUCA.
HOGENOMiHOG000229394.
KOiK01622.
OMAiHLVAGWM.
OrthoDBiPOG093Z0396.

Family and domain databases

HAMAPiMF_02067. FBP_aldolase_phosphatase. 1 hit.
InterProiView protein in InterPro
IPR002803. FBPase_V.
PfamiView protein in Pfam
PF01950. FBPase_3. 1 hit.
PIRSFiPIRSF015647. FBPtase_archl. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014260. FBPase_V. 1 hit.
SUPFAMiSSF111249. SSF111249. 1 hit.

Sequencei

Sequence statusi: Complete.

A0B8T5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVTVSLIK ADVGGWPGHA SVHPALIDKA EEVLSEAKSA GTLIDFKVMA
60 70 80 90 100
CGDDLELLMT HRLGTDEAEI HAIAWETFEK ATAEAKSLKL YGAGQDLLCD
110 120 130 140 150
AFSGNIRGMG PGVAEMQFTE RDAEPLVAFM MDKTEPGAFN LPIFRMFADP
160 170 180 190 200
FNTAGLVIDP SLHNGFIFEI WDILDHKKVF MSCPEEMYNI LALIGAKSRY
210 220 230 240 250
VIKRVYPKPG GKLPSEEPVA VVSTEKLYQT AGTYVGKDDP VALVRAQSGL
260 270 280 290 300
PALGEVLEPF ALGHLVSGWM RGSHNGPLMP CAFEDAHPTR FDGPPRVIAA
310 320 330 340 350
GFQLCNGLLI GPVDLFADVA FDLTRMRVLE ITDYMRAHGP FEPHRLPLED
360 370
MEYTTLPHVL KRLAERFEPA E
Length:371
Mass (Da):40,710
Last modified:November 28, 2006 - v1
Checksum:i8A9F2AC03FA7D6EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA. Translation: ABK15109.1.

Genome annotation databases

EnsemblBacteriaiABK15109; ABK15109; Mthe_1333.
KEGGimtp:Mthe_1333.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA. Translation: ABK15109.1.

3D structure databases

ProteinModelPortaliA0B8T5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349307.Mthe_1333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK15109; ABK15109; Mthe_1333.
KEGGimtp:Mthe_1333.

Phylogenomic databases

eggNOGiarCOG04180. Archaea.
COG1980. LUCA.
HOGENOMiHOG000229394.
KOiK01622.
OMAiHLVAGWM.
OrthoDBiPOG093Z0396.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciMTHE349307:G12X8-1343-MONOMER.

Family and domain databases

HAMAPiMF_02067. FBP_aldolase_phosphatase. 1 hit.
InterProiView protein in InterPro
IPR002803. FBPase_V.
PfamiView protein in Pfam
PF01950. FBPase_3. 1 hit.
PIRSFiPIRSF015647. FBPtase_archl. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014260. FBPase_V. 1 hit.
SUPFAMiSSF111249. SSF111249. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA0B8T5_METTP
AccessioniPrimary (citable) accession number: A0B8T5
Entry historyiIntegrated into UniProtKB/TrEMBL: November 28, 2006
Last sequence update: November 28, 2006
Last modified: April 12, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.