Reviewed, UniProtKB/Swiss-Prot A0B8K5 (DP2L_METTP)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	Mthe_1250

Organism

Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]

Taxonomic identifier

349307 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosaetaceae › Methanosaeta

Protein attributes

Sequence length	1109 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1109	1109	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000294691

Sequences

	Sequence		Length	Mass (Da)
	A0B8K5-1 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 9CAFADACAC8D75C2	FASTA	1,109	122,895
	10 20 30 40 50 60 MSAEYFERLE RGLREEMEIA RRARSKGLDP ETDVEIPIAV DLAERVEKLV SIPGVAERIR 70 80 90 100 110 120 EFEVQGLSRE EAALAIGRDF AEGRLGKGAS RLDAIDMAIR TSVALLTEGV VAAPIEGIAR 130 140 150 160 170 180 VGLGKNDDGS EYLKVYYAGP IRSAGGTAQA LSVLVADYVR RSMGIAPYKP TDEEIERYVE 190 200 210 220 230 240 EIGLYKRLQH LQYTPSDDEI RTIIRNCPVC IDGEPTEEEE VSGYRDLPRV ETNRVRGGIA 250 260 270 280 290 300 LVSAEGIALK RPKIKKHVSK LGIDGWGWLD VLGGEKKDGG GSSGKFLRDL IAGRPVFSHP 310 320 330 340 350 360 SRPGGFRLRY GRSRNTGLAA AGINPATMMV LNQFLAPGTQ IKVEQPGKAA AVAPVSSIEG 370 380 390 400 410 420 PTVRLTNGDV VRIDDPSSFP WISSPEALRR NISKIIDLGE ILVSFGDFME NNRPLAPASY 430 440 450 460 470 480 TFEWWAEEFR RAGGDPAGME HVDGRTAIRL SRDFGVPLHP DHTYLWHDIT LDELDLLADA 490 500 510 520 530 540 ASDGEIDGDA LLMDMREDVK EVLEKLLVHH KVRDGMIVVE DALPLLLCLG VDETGRRWRR 550 560 570 580 590 600 EDLNADNALS AVNQLSGLVI RARAPTRIGC RMGRPEKSDR REMRPPPHVL FPTGSEGGKS 610 620 630 640 650 660 RLVNEAAEHG FIEVVVERRR CPSCGKEGFA FRCECGAHTE RVRACLSCGV RADDKCPRCG 670 680 690 700 710 720 TETSAATRMR IDVKGTLARA LESLGERVDN IRGVMGLTSR DSTPEPLEKG VLRAKHGVFI 730 740 750 760 770 780 FKDGTSRYDL TDLPLTHFRP REIGTSVDAL LDLGYTHDIH GKPLESDDQI LELKVQDVVL 790 800 810 820 830 840 CRNAGDWLLR VSKFIDDLLV KFYGLEPYYN AERPEDLIGR LMIGLAPHTS AGVLCRLIGF 850 860 870 880 890 900 TRASAGYAHP YFHAAKRRNC DGDEDCVMLL MDALLNFSRS YLPEKRGGKM DAPLVLTTRI 910 920 930 940 950 960 NPAEVDKEAH NLDLSFIYPL EFYEATLRNA NPKDVEGLID LVSKRLGSEG QYCGFGFTHD 970 980 990 1000 1010 1020 TDDIAGGPRN SSYKTLETMI DKMKSQLELA KLIRAVDATD VAERVINSHF LPDLMGNLRA 1030 1040 1050 1060 1070 1080 FSRQSVRCVK CNAKYRRPPL SESCPKCGGR LVLTVHEGSV RKYLEVSMKV AEEYGVSSYT 1090 1100 RQRLELIQME IESLFRSDKV KQTGLADFV

References

[1]

"Complete sequence of Methanosaeta thermophila PT." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Smith K.S., Ingram-Smith C., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000477 Genomic DNA. Translation: ABK15029.1.
RefSeq	YP_843669.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0B8K5.
Genome annotation databases
GeneID	4463181.
GenomeReviews	Gene locus Mthe_1250 in contig CP000477_GR.
KEGG	mtp:Mthe_1250.
NMPDR	fig|349307.7.peg.1296.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04008.
HOGENOM	HBG497074.
OMA	NCDGDED.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METTP

Accession

Primary (citable) accession number: A0B8K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	November 28, 2006
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)