A0B7Z0 (AROA_METTP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	Mthe_1029

Organism

Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]

Taxonomic identifier

349307 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosaetaceae › Methanosaeta

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Probable 3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000012452

Sequences

	Sequence		Length	Mass (Da)
	A0B7Z0 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: AFA2FAF63B4A2806	FASTA	421	44,361
	10 20 30 40 50 60 MIVSVSRSSI SGTVCAPPSK SYTHRAVLIT ALSDSGCVHR PLISADTRAT ISACDAFGAD 70 80 90 100 110 120 VKLRGDSLEI QGVSGAPRTP ENVIDVLNSG TTLRFMSAVA ALTDGAVLTG DSSIRSRPNG 130 140 150 160 170 180 PLLKALNELG AEAFSIRGND RAPLVIRGRL RGGSTSLDGS VSSQFLSALL IACPLSSGET 190 200 210 220 230 240 TISIKGELKS RPYAEMTLDI LRKAGAEICT DGDIFRMRGG QSYRLAEYTV PGDFSSASYP 250 260 270 280 290 300 LAAAALAGSA TVEGLFPSRQ GDSAIVDILR EMGAEVSWDM ESGEVRVSGA DLRGREIDAS 310 320 330 340 350 360 QTPDLVPTLA VLGAVAEGRT VIKNAEHVRH KETDRIHAMA VELKKMGANI RERPDGLEID 370 380 390 400 410 420 GGDLHGADLH GYHDHRIVMA LTLAGIVAGD TRIDTAESVD VSYPGFFEDM RRLGANVRAS T

References

[1]

"Complete sequence of Methanosaeta thermophila PT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Smith K.S., Ingram-Smith C., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6194 / PT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000477 Genomic DNA. Translation: ABK14814.1.
RefSeq	YP_843454.1. NC_008553.1.
3D structure databases
ProteinModelPortal	A0B7Z0.
ModBase	Search...
Protein-protein interaction databases
STRING	A0B7Z0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4462778.
GenomeReviews	Gene locus Mthe_1029 in contig CP000477_GR.
KEGG	mtp:Mthe_1029.
NMPDR	fig|349307.7.peg.1068.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05032.
HOGENOM	HBG646626.
OMA	RLRIKEC.
PhylomeDB	A0B7Z0.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	MTHE349307:MTHE_1029-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. False negative. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_METTP

Accession

Primary (citable) accession number: A0B7Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 28, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program