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A0B7P3

- SYI_METTP

UniProt

A0B7P3 - SYI_METTP

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (28 Nov 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei599 – 5991ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMMET349307:GHKI-959-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Mthe_0929
    OrganismiMethanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
    Taxonomic identifieri349307 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta
    ProteomesiUP000000674: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10491049Isoleucine--tRNA ligasePRO_1000022157Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi349307.Mthe_0929.

    Structurei

    3D structure databases

    ProteinModelPortaliA0B7P3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi596 – 6005"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiKPVHWCL.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0B7P3-1 [UniParc]FASTAAdd to Basket

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    MIVEVPGQYN PKNVEDEVRE FWHREDTYHK VRRLRSGGRR FFFVDGPPYT     50
    TGRIHLGTAW NKVIKDSILR YMSMRGYDLK DRAGWDMHGL PIEVKVEEHL 100
    GFRSKRDIES YGVDRFIEQC KSFALRQKDE MTEQFKSLGV WLDWDNPYMT 150
    LKNEYIEAAW WTLKRAHERG LLERGLRVVN WCPRCQTAIA DSEVEYWDES 200
    DPSIYVKFPV EGEDNTYIVI WTTTPWTIPA NVAVAVHKDF MYSKVRAWRR 250
    DGTYEILIMA TDLIENVLKQ GRYVDYEILE SMRGEDLLSL TYKNPLQDLV 300
    PAQRDIVHRV HLADFVTAEN TGIVHIAPGH GLEDYELGLE KRLPIFCPVG 350
    EDGRYTSEAG KKYEGKYVRD ANPEVVEDLM ERGALLASGE LVHRYGHCWR 400
    CKTPIIFIAT RQWFIGISEL RDQMLKEIER VSWYPDWAGS ARFKDWISNA 450
    RDWCISRQRY WGIPLPIWIC RSCGAMDVIG TAAELEARAG RPVEDLHRPA 500
    VDDVRLRCSC GGAMERVPDV FDVWFDSAVA SWATLNFPRD EEEFRVWWPA 550
    DFITEGHDQT RGWFYSQLGA SMVAFGRAPY KSVLMHGFTL DEQGRKMSKS 600
    IGNVVHPEDV VERYGADTLR FYVLSSNAPW EDLHFSWEGA MNVNRMLNIL 650
    WNAYRFPLPY MVLDRFDISK ADTSKYDLRP EDRWIISRVN SLAKEIEENM 700
    SGYMLHRATR SLQEFVLEDL SRWYIQLVRP RTWIETEDPD KLAAYATLYN 750
    VMSTLVKLMA PFTPFLAESI YQNLVRGLDP SAPESVHMCD WPGYREDLID 800
    KGLEESMSYV REISEAAANA RQKGGRKLRW PVSRIIIASD EQLDLRDLLH 850
    VLRTQTNSKE VIILPPGEKP EMNLEISVVQ KKIGPVFKGE SRDVVEALTS 900
    MNPKEVKRII ESGEPLEWKG RSYMITEEMV EFREIPPANL IPAEFSKGTV 950
    YVDVSLTDEL RAEGYAREII RRIQDMRKDL DLKVDEMIRV SVALNSNEVV 1000
    DLVSGWRDYI SSEVRATLLR IGNDLDLEGE LTKDWEVDGV KIRVSVARA 1049
    Length:1,049
    Mass (Da):121,605
    Last modified:November 28, 2006 - v1
    Checksum:iF491CBA9FA700B1E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000477 Genomic DNA. Translation: ABK14717.1.
    RefSeqiYP_843357.1. NC_008553.1.

    Genome annotation databases

    EnsemblBacteriaiABK14717; ABK14717; Mthe_0929.
    GeneIDi4463322.
    KEGGimtp:Mthe_0929.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000477 Genomic DNA. Translation: ABK14717.1 .
    RefSeqi YP_843357.1. NC_008553.1.

    3D structure databases

    ProteinModelPortali A0B7P3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349307.Mthe_0929.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK14717 ; ABK14717 ; Mthe_0929 .
    GeneIDi 4463322.
    KEGGi mtp:Mthe_0929.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi KPVHWCL.

    Enzyme and pathway databases

    BioCyci MMET349307:GHKI-959-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 6194 / PT.

    Entry informationi

    Entry nameiSYI_METTP
    AccessioniPrimary (citable) accession number: A0B7P3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3