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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT) (Methanosaeta thermophila)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei599ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mthe_0929
OrganismiMethanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT) (Methanosaeta thermophila)
Taxonomic identifieri349307 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanothrix
Proteomesi
  • UP000000674 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221571 – 1049Isoleucine--tRNA ligaseAdd BLAST1049

Proteomic databases

PRIDEiA0B7P3

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi349307.Mthe_0929

Structurei

3D structure databases

ProteinModelPortaliA0B7P3
SMRiA0B7P3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 58"HIGH" regionAdd BLAST11
Motifi596 – 600"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A0B7P3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVEVPGQYN PKNVEDEVRE FWHREDTYHK VRRLRSGGRR FFFVDGPPYT
60 70 80 90 100
TGRIHLGTAW NKVIKDSILR YMSMRGYDLK DRAGWDMHGL PIEVKVEEHL
110 120 130 140 150
GFRSKRDIES YGVDRFIEQC KSFALRQKDE MTEQFKSLGV WLDWDNPYMT
160 170 180 190 200
LKNEYIEAAW WTLKRAHERG LLERGLRVVN WCPRCQTAIA DSEVEYWDES
210 220 230 240 250
DPSIYVKFPV EGEDNTYIVI WTTTPWTIPA NVAVAVHKDF MYSKVRAWRR
260 270 280 290 300
DGTYEILIMA TDLIENVLKQ GRYVDYEILE SMRGEDLLSL TYKNPLQDLV
310 320 330 340 350
PAQRDIVHRV HLADFVTAEN TGIVHIAPGH GLEDYELGLE KRLPIFCPVG
360 370 380 390 400
EDGRYTSEAG KKYEGKYVRD ANPEVVEDLM ERGALLASGE LVHRYGHCWR
410 420 430 440 450
CKTPIIFIAT RQWFIGISEL RDQMLKEIER VSWYPDWAGS ARFKDWISNA
460 470 480 490 500
RDWCISRQRY WGIPLPIWIC RSCGAMDVIG TAAELEARAG RPVEDLHRPA
510 520 530 540 550
VDDVRLRCSC GGAMERVPDV FDVWFDSAVA SWATLNFPRD EEEFRVWWPA
560 570 580 590 600
DFITEGHDQT RGWFYSQLGA SMVAFGRAPY KSVLMHGFTL DEQGRKMSKS
610 620 630 640 650
IGNVVHPEDV VERYGADTLR FYVLSSNAPW EDLHFSWEGA MNVNRMLNIL
660 670 680 690 700
WNAYRFPLPY MVLDRFDISK ADTSKYDLRP EDRWIISRVN SLAKEIEENM
710 720 730 740 750
SGYMLHRATR SLQEFVLEDL SRWYIQLVRP RTWIETEDPD KLAAYATLYN
760 770 780 790 800
VMSTLVKLMA PFTPFLAESI YQNLVRGLDP SAPESVHMCD WPGYREDLID
810 820 830 840 850
KGLEESMSYV REISEAAANA RQKGGRKLRW PVSRIIIASD EQLDLRDLLH
860 870 880 890 900
VLRTQTNSKE VIILPPGEKP EMNLEISVVQ KKIGPVFKGE SRDVVEALTS
910 920 930 940 950
MNPKEVKRII ESGEPLEWKG RSYMITEEMV EFREIPPANL IPAEFSKGTV
960 970 980 990 1000
YVDVSLTDEL RAEGYAREII RRIQDMRKDL DLKVDEMIRV SVALNSNEVV
1010 1020 1030 1040
DLVSGWRDYI SSEVRATLLR IGNDLDLEGE LTKDWEVDGV KIRVSVARA
Length:1,049
Mass (Da):121,605
Last modified:November 28, 2006 - v1
Checksum:iF491CBA9FA700B1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA Translation: ABK14717.1

Genome annotation databases

EnsemblBacteriaiABK14717; ABK14717; Mthe_0929
KEGGimtp:Mthe_0929

Similar proteinsi

Entry informationi

Entry nameiSYI_METTP
AccessioniPrimary (citable) accession number: A0B7P3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 28, 2006
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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