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A0B7P3

- SYI_METTP

UniProt

A0B7P3 - SYI_METTP

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Mthe_0929
Organism
Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei599 – 5991ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMET349307:GHKI-959-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Mthe_0929
OrganismiMethanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
Taxonomic identifieri349307 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta
ProteomesiUP000000674: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10491049Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022157Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi349307.Mthe_0929.

Structurei

3D structure databases

ProteinModelPortaliA0B7P3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi596 – 6005"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0B7P3-1 [UniParc]FASTAAdd to Basket

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MIVEVPGQYN PKNVEDEVRE FWHREDTYHK VRRLRSGGRR FFFVDGPPYT     50
TGRIHLGTAW NKVIKDSILR YMSMRGYDLK DRAGWDMHGL PIEVKVEEHL 100
GFRSKRDIES YGVDRFIEQC KSFALRQKDE MTEQFKSLGV WLDWDNPYMT 150
LKNEYIEAAW WTLKRAHERG LLERGLRVVN WCPRCQTAIA DSEVEYWDES 200
DPSIYVKFPV EGEDNTYIVI WTTTPWTIPA NVAVAVHKDF MYSKVRAWRR 250
DGTYEILIMA TDLIENVLKQ GRYVDYEILE SMRGEDLLSL TYKNPLQDLV 300
PAQRDIVHRV HLADFVTAEN TGIVHIAPGH GLEDYELGLE KRLPIFCPVG 350
EDGRYTSEAG KKYEGKYVRD ANPEVVEDLM ERGALLASGE LVHRYGHCWR 400
CKTPIIFIAT RQWFIGISEL RDQMLKEIER VSWYPDWAGS ARFKDWISNA 450
RDWCISRQRY WGIPLPIWIC RSCGAMDVIG TAAELEARAG RPVEDLHRPA 500
VDDVRLRCSC GGAMERVPDV FDVWFDSAVA SWATLNFPRD EEEFRVWWPA 550
DFITEGHDQT RGWFYSQLGA SMVAFGRAPY KSVLMHGFTL DEQGRKMSKS 600
IGNVVHPEDV VERYGADTLR FYVLSSNAPW EDLHFSWEGA MNVNRMLNIL 650
WNAYRFPLPY MVLDRFDISK ADTSKYDLRP EDRWIISRVN SLAKEIEENM 700
SGYMLHRATR SLQEFVLEDL SRWYIQLVRP RTWIETEDPD KLAAYATLYN 750
VMSTLVKLMA PFTPFLAESI YQNLVRGLDP SAPESVHMCD WPGYREDLID 800
KGLEESMSYV REISEAAANA RQKGGRKLRW PVSRIIIASD EQLDLRDLLH 850
VLRTQTNSKE VIILPPGEKP EMNLEISVVQ KKIGPVFKGE SRDVVEALTS 900
MNPKEVKRII ESGEPLEWKG RSYMITEEMV EFREIPPANL IPAEFSKGTV 950
YVDVSLTDEL RAEGYAREII RRIQDMRKDL DLKVDEMIRV SVALNSNEVV 1000
DLVSGWRDYI SSEVRATLLR IGNDLDLEGE LTKDWEVDGV KIRVSVARA 1049
Length:1,049
Mass (Da):121,605
Last modified:November 28, 2006 - v1
Checksum:iF491CBA9FA700B1E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000477 Genomic DNA. Translation: ABK14717.1.
RefSeqiYP_843357.1. NC_008553.1.

Genome annotation databases

EnsemblBacteriaiABK14717; ABK14717; Mthe_0929.
GeneIDi4463322.
KEGGimtp:Mthe_0929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000477 Genomic DNA. Translation: ABK14717.1 .
RefSeqi YP_843357.1. NC_008553.1.

3D structure databases

ProteinModelPortali A0B7P3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 349307.Mthe_0929.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK14717 ; ABK14717 ; Mthe_0929 .
GeneIDi 4463322.
KEGGi mtp:Mthe_0929.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MMET349307:GHKI-959-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 6194 / PT.

Entry informationi

Entry nameiSYI_METTP
AccessioniPrimary (citable) accession number: A0B7P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 28, 2006
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi