Reviewed, UniProtKB/Swiss-Prot A0B7F9 (DNLI_METTP)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	Mthe_0844

Organism

Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]

Taxonomic identifier

349307 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosaetaceae › Methanosaeta

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	DNA ligase HAMAP MF_00407		PRO_1000049873
Sites
	Active site	246	1	N6-AMP-lysine intermediate By similarity
	Binding site	244	1	ATP By similarity
	Binding site	251	1	ATP By similarity
	Binding site	266	1	ATP By similarity
	Binding site	296	1	ATP By similarity
	Binding site	336	1	ATP By similarity
	Binding site	408	1	ATP By similarity
	Binding site	414	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0B7F9-1 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 4A0865A5F8711501	FASTA	552	61,188
	10 20 30 40 50 60 MTGFARFAEL CERISQTSGS LEKTDILASF LSDLESDDLR IVAGFVMGVV IPGTELGVGP 70 80 90 100 110 120 SLLYESISRA TGLSSDAVNE LLRATGDPGL VAYRAVERRK PLTLAAFSGS EGLEVQDVYQ 130 140 150 160 170 180 RFLSIAKASG RGSQEIRVKN LQYMFSEASP LEAKYIARLA MEDMRIGVGE GLVRDAIAKA 190 200 210 220 230 240 FGVSKEDVER AYNLTNDLGL VAEYAKLGRL NELGISINRP IKMMLAQIGE SIEASLAEGA 250 260 270 280 290 300 TAVEWKFDGA RVQIHKDKGN VRIFSRRLED VTSSLPEIRE IVRGHVRART AILDGEAVAT 310 320 330 340 350 360 GEDGRPLPFQ EILRRFRRKY GVARTAKTIP LKLHLFDIIY MDGASLLDEP LEERRRVLVS 370 380 390 400 410 420 VADPEIIAEQ VVTSDVHRVE EIYREALAAG HEGVMLKNPS STYTPGKRGK NWLKIKPLLE 430 440 450 460 470 480 SLDLVVIGAR WGEGKRANLL GSYRLACIDT DTGELKDVGW VATGITDEML AELTELFREL 490 500 510 520 530 540 IVKENGMEVE VHPEIVFEVG YEEIQRSPNY SSGYALRFPR LIAVRDDKSP SEADTLERIG 550 EIYRLQRGRS KK

References

[1]

"Complete sequence of Methanosaeta thermophila PT." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Smith K.S., Ingram-Smith C., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000477 Genomic DNA. Translation: ABK14633.1.
RefSeq	YP_843273.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4463057.
GenomeReviews	Gene locus Mthe_0844 in contig CP000477_GR.
KEGG	mtp:Mthe_0844.
NMPDR	fig|349307.7.peg.872.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A0B7F9. SNLHLGA.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_METTP

Accession

Primary (citable) accession number: A0B7F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 28, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)