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Protein

DNA ligase

Gene

lig

Organism
Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei244 – 2441ATPUniRule annotation
Active sitei246 – 2461N6-AMP-lysine intermediateUniRule annotation
Binding sitei251 – 2511ATPUniRule annotation
Binding sitei266 – 2661ATPUniRule annotation
Binding sitei296 – 2961ATPUniRule annotation
Binding sitei336 – 3361ATPUniRule annotation
Binding sitei408 – 4081ATPUniRule annotation
Binding sitei414 – 4141ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMET349307:GHKI-873-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:Mthe_0844
OrganismiMethanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
Taxonomic identifieri349307 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta
ProteomesiUP000000674 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 552552DNA ligasePRO_1000049873Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi349307.Mthe_0844.

Structurei

3D structure databases

ProteinModelPortaliA0B7F9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK10747.
OMAiNPVESRY.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0B7F9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGFARFAEL CERISQTSGS LEKTDILASF LSDLESDDLR IVAGFVMGVV
60 70 80 90 100
IPGTELGVGP SLLYESISRA TGLSSDAVNE LLRATGDPGL VAYRAVERRK
110 120 130 140 150
PLTLAAFSGS EGLEVQDVYQ RFLSIAKASG RGSQEIRVKN LQYMFSEASP
160 170 180 190 200
LEAKYIARLA MEDMRIGVGE GLVRDAIAKA FGVSKEDVER AYNLTNDLGL
210 220 230 240 250
VAEYAKLGRL NELGISINRP IKMMLAQIGE SIEASLAEGA TAVEWKFDGA
260 270 280 290 300
RVQIHKDKGN VRIFSRRLED VTSSLPEIRE IVRGHVRART AILDGEAVAT
310 320 330 340 350
GEDGRPLPFQ EILRRFRRKY GVARTAKTIP LKLHLFDIIY MDGASLLDEP
360 370 380 390 400
LEERRRVLVS VADPEIIAEQ VVTSDVHRVE EIYREALAAG HEGVMLKNPS
410 420 430 440 450
STYTPGKRGK NWLKIKPLLE SLDLVVIGAR WGEGKRANLL GSYRLACIDT
460 470 480 490 500
DTGELKDVGW VATGITDEML AELTELFREL IVKENGMEVE VHPEIVFEVG
510 520 530 540 550
YEEIQRSPNY SSGYALRFPR LIAVRDDKSP SEADTLERIG EIYRLQRGRS

KK
Length:552
Mass (Da):61,188
Last modified:November 28, 2006 - v1
Checksum:i4A0865A5F8711501
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA. Translation: ABK14633.1.
RefSeqiWP_011696029.1. NC_008553.1.
YP_843273.1. NC_008553.1.

Genome annotation databases

EnsemblBacteriaiABK14633; ABK14633; Mthe_0844.
GeneIDi4463057.
KEGGimtp:Mthe_0844.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA. Translation: ABK14633.1.
RefSeqiWP_011696029.1. NC_008553.1.
YP_843273.1. NC_008553.1.

3D structure databases

ProteinModelPortaliA0B7F9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349307.Mthe_0844.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK14633; ABK14633; Mthe_0844.
GeneIDi4463057.
KEGGimtp:Mthe_0844.

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK10747.
OMAiNPVESRY.

Enzyme and pathway databases

BioCyciMMET349307:GHKI-873-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 6194 / PT.

Entry informationi

Entry nameiDNLI_METTP
AccessioniPrimary (citable) accession number: A0B7F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 28, 2006
Last modified: April 1, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.