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A0B7F9 (DNLI_METTP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:Mthe_0844
OrganismMethanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]
Taxonomic identifier349307 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta

Protein attributes

Sequence length552 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 552552DNA ligase HAMAP-Rule MF_00407
PRO_1000049873

Sites

Active site2461N6-AMP-lysine intermediate By similarity
Binding site2441ATP By similarity
Binding site2511ATP By similarity
Binding site2661ATP By similarity
Binding site2961ATP By similarity
Binding site3361ATP By similarity
Binding site4081ATP By similarity
Binding site4141ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0B7F9 [UniParc].

Last modified November 28, 2006. Version 1.
Checksum: 4A0865A5F8711501

FASTA55261,188
        10         20         30         40         50         60 
MTGFARFAEL CERISQTSGS LEKTDILASF LSDLESDDLR IVAGFVMGVV IPGTELGVGP 

        70         80         90        100        110        120 
SLLYESISRA TGLSSDAVNE LLRATGDPGL VAYRAVERRK PLTLAAFSGS EGLEVQDVYQ 

       130        140        150        160        170        180 
RFLSIAKASG RGSQEIRVKN LQYMFSEASP LEAKYIARLA MEDMRIGVGE GLVRDAIAKA 

       190        200        210        220        230        240 
FGVSKEDVER AYNLTNDLGL VAEYAKLGRL NELGISINRP IKMMLAQIGE SIEASLAEGA 

       250        260        270        280        290        300 
TAVEWKFDGA RVQIHKDKGN VRIFSRRLED VTSSLPEIRE IVRGHVRART AILDGEAVAT 

       310        320        330        340        350        360 
GEDGRPLPFQ EILRRFRRKY GVARTAKTIP LKLHLFDIIY MDGASLLDEP LEERRRVLVS 

       370        380        390        400        410        420 
VADPEIIAEQ VVTSDVHRVE EIYREALAAG HEGVMLKNPS STYTPGKRGK NWLKIKPLLE 

       430        440        450        460        470        480 
SLDLVVIGAR WGEGKRANLL GSYRLACIDT DTGELKDVGW VATGITDEML AELTELFREL 

       490        500        510        520        530        540 
IVKENGMEVE VHPEIVFEVG YEEIQRSPNY SSGYALRFPR LIAVRDDKSP SEADTLERIG 

       550 
EIYRLQRGRS KK 

« Hide

References

[1]"Complete sequence of Methanosaeta thermophila PT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Smith K.S., Ingram-Smith C., Richardson P.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6194 / PT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000477 Genomic DNA. Translation: ABK14633.1.
RefSeqYP_843273.1. NC_008553.1.

3D structure databases

ProteinModelPortalA0B7F9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349307.Mthe_0844.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK14633; ABK14633; Mthe_0844.
GeneID4463057.
KEGGmtp:Mthe_0844.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMANPVESRY.
ProtClustDBCLSK568925.

Enzyme and pathway databases

BioCycMMET349307:GHKI-873-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_METTP
AccessionPrimary (citable) accession number: A0B7F9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 28, 2006
Last modified: February 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families