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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei632 – 6321ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMET349307:GHKI-828-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Mthe_0802
OrganismiMethanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
Taxonomic identifieri349307 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta
ProteomesiUP000000674 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 950950Leucine--tRNA ligasePRO_0000334847Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi349307.Mthe_0802.

Structurei

3D structure databases

ProteinModelPortaliA0B7B7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi629 – 6335"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiDAYINIF.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0B7B7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRNEYSAHE IEAKWQRIWE EEGVFHAEPD SRKKFFLTIP YPYLNGNLHA
60 70 80 90 100
GHTRTFTIGD AIARYHRMLG ENVLFPMAFH ATGTPIVGLS ELIANRDPLI
110 120 130 140 150
WDVYTRLHGI PEEELEKLTT PEAIVDYFRK QAKLAMRSIG YSIDWRREFT
160 170 180 190 200
TTDPAYNRFI EWQFGILREK GYVTKGSHPV RWCPNDQNPV EDHDILRGED
210 220 230 240 250
ATILDFTLIK FRLDDKVLPC ATLRPETVFG VTNLWVNPNV VHYIARVNDE
260 270 280 290 300
VWIVSPQAYH KLTFTDRSVK KIGEIPGEEL IGKKARNPVT GDEIIILPAT
310 320 330 340 350
FVDPDSGSGI VMSVPAHAPL DYLALRDLYD ADLSKYGITE DLRKIKFISL
360 370 380 390 400
ISVPEYGEFP AVDAVNELGV KDQNDPKAEE ATKLVYRREF HNGVLKEITG
410 420 430 440 450
RYAGTPVHRI KDILLQDLIN QGVAEIFYEF SETPVICRCG ARCVVKMVRD
460 470 480 490 500
QWFLEYSDPV WKSRVLECLA GMQIIPEEMR AEFINKIDWL KDKACARRKG
510 520 530 540 550
LGTRLPWDRE WLIESLADST IYMAFYILAK YVNAGMKIDR LVPQFFDYIF
560 570 580 590 600
LGKGTPEEVS SLTGVDVDTV RRIREDFEYW YPVDLRTSGK DLVANHLLFF
610 620 630 640 650
LYHHVAIFPE SLWPRAIAVN GFVSLEGQKM SKSRGPILTL KQAVAENGAD
660 670 680 690 700
VTRLYILANA EYTQDADWRN DGAQATRGQV ERFYTLAREI IERNDIDESA
710 720 730 740 750
ELTLIDRWML SRLQRRIIET TDALNNIQTR RALQSAFYHM LNDLRWYERR
760 770 780 790 800
GGRNQLRRIL NVWVRLMAPF TPHICEEIWQ NIGEGYVSRA PWPVPDASLI
810 820 830 840 850
DEQAERAEAY LEQTQKDIEE IIRVTKTKPR RIVLYTTPVW KREMLRLALE
860 870 880 890 900
VSKGGRLDMG ALMKSAMGHP EIQSHKKDAP KYGGKLAKSV HALSGDVLAL
910 920 930 940 950
DELGILSRER EYLSQAFGCP VEVYSADNPP YDPKGRAQNA EPGRPAIYIE
Length:950
Mass (Da):109,145
Last modified:November 28, 2006 - v1
Checksum:iDFCBC447C11687B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA. Translation: ABK14591.1.
RefSeqiWP_011695987.1. NC_008553.1.
YP_843231.1. NC_008553.1.

Genome annotation databases

EnsemblBacteriaiABK14591; ABK14591; Mthe_0802.
GeneIDi4461989.
KEGGimtp:Mthe_0802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000477 Genomic DNA. Translation: ABK14591.1.
RefSeqiWP_011695987.1. NC_008553.1.
YP_843231.1. NC_008553.1.

3D structure databases

ProteinModelPortaliA0B7B7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349307.Mthe_0802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK14591; ABK14591; Mthe_0802.
GeneIDi4461989.
KEGGimtp:Mthe_0802.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiDAYINIF.

Enzyme and pathway databases

BioCyciMMET349307:GHKI-828-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 6194 / PT.

Entry informationi

Entry nameiSYL_METTP
AccessioniPrimary (citable) accession number: A0B7B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 28, 2006
Last modified: July 22, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.