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A0B7B7 (SYL_METTP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Mthe_0802
OrganismMethanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]
Taxonomic identifier349307 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta

Protein attributes

Sequence length950 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 950950Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334847

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif629 – 6335"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0B7B7 [UniParc].

Last modified November 28, 2006. Version 1.
Checksum: DFCBC447C11687B6

FASTA950109,145
        10         20         30         40         50         60 
MLRNEYSAHE IEAKWQRIWE EEGVFHAEPD SRKKFFLTIP YPYLNGNLHA GHTRTFTIGD 

        70         80         90        100        110        120 
AIARYHRMLG ENVLFPMAFH ATGTPIVGLS ELIANRDPLI WDVYTRLHGI PEEELEKLTT 

       130        140        150        160        170        180 
PEAIVDYFRK QAKLAMRSIG YSIDWRREFT TTDPAYNRFI EWQFGILREK GYVTKGSHPV 

       190        200        210        220        230        240 
RWCPNDQNPV EDHDILRGED ATILDFTLIK FRLDDKVLPC ATLRPETVFG VTNLWVNPNV 

       250        260        270        280        290        300 
VHYIARVNDE VWIVSPQAYH KLTFTDRSVK KIGEIPGEEL IGKKARNPVT GDEIIILPAT 

       310        320        330        340        350        360 
FVDPDSGSGI VMSVPAHAPL DYLALRDLYD ADLSKYGITE DLRKIKFISL ISVPEYGEFP 

       370        380        390        400        410        420 
AVDAVNELGV KDQNDPKAEE ATKLVYRREF HNGVLKEITG RYAGTPVHRI KDILLQDLIN 

       430        440        450        460        470        480 
QGVAEIFYEF SETPVICRCG ARCVVKMVRD QWFLEYSDPV WKSRVLECLA GMQIIPEEMR 

       490        500        510        520        530        540 
AEFINKIDWL KDKACARRKG LGTRLPWDRE WLIESLADST IYMAFYILAK YVNAGMKIDR 

       550        560        570        580        590        600 
LVPQFFDYIF LGKGTPEEVS SLTGVDVDTV RRIREDFEYW YPVDLRTSGK DLVANHLLFF 

       610        620        630        640        650        660 
LYHHVAIFPE SLWPRAIAVN GFVSLEGQKM SKSRGPILTL KQAVAENGAD VTRLYILANA 

       670        680        690        700        710        720 
EYTQDADWRN DGAQATRGQV ERFYTLAREI IERNDIDESA ELTLIDRWML SRLQRRIIET 

       730        740        750        760        770        780 
TDALNNIQTR RALQSAFYHM LNDLRWYERR GGRNQLRRIL NVWVRLMAPF TPHICEEIWQ 

       790        800        810        820        830        840 
NIGEGYVSRA PWPVPDASLI DEQAERAEAY LEQTQKDIEE IIRVTKTKPR RIVLYTTPVW 

       850        860        870        880        890        900 
KREMLRLALE VSKGGRLDMG ALMKSAMGHP EIQSHKKDAP KYGGKLAKSV HALSGDVLAL 

       910        920        930        940        950 
DELGILSRER EYLSQAFGCP VEVYSADNPP YDPKGRAQNA EPGRPAIYIE 

« Hide

References

[1]"Complete sequence of Methanosaeta thermophila PT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Smith K.S., Ingram-Smith C., Richardson P.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6194 / PT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000477 Genomic DNA. Translation: ABK14591.1.
RefSeqYP_843231.1. NC_008553.1.

3D structure databases

ProteinModelPortalA0B7B7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349307.Mthe_0802.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK14591; ABK14591; Mthe_0802.
GeneID4461989.
KEGGmtp:Mthe_0802.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAHAPFDYL.

Enzyme and pathway databases

BioCycMMET349307:GHKI-828-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METTP
AccessionPrimary (citable) accession number: A0B7B7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 28, 2006
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries