A0B6X3 (SYA_METTP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Mthe_0657

Organism

Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]

Taxonomic identifier

349307 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosaetaceae › Methanosaeta

Protein attributes

Sequence length	913 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 913	913	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000347887
Sites
	Metal binding	600	1	Zinc By similarity
	Metal binding	604	1	Zinc By similarity
	Metal binding	703	1	Zinc By similarity
	Metal binding	707	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0B6X3 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 04FB68F30E7F63D1	FASTA	913	101,963
	10 20 30 40 50 60 MFPEDEYQLE FFRTEGFVRK VCESCGGSFW TRDASRRTCG DPPCDPYSFI GSPVFREMEL 70 80 90 100 110 120 DSMREHYLSF FEAHGHTRVQ RYPVVARWRD DIYLTIASIA DFQPFVTSGQ VPPPANPLTI 130 140 150 160 170 180 SQPCIRLDDL DSVGRSGRHL TTFEMMAHHV FNTKEHEIYW KDRTVELCDE LLLGLGVDPE 190 200 210 220 230 240 SITYKESPWA GGGNAGPSLE VLVGGLELAT LVFMNLRLDS SGEYVIKGER YSRMDNYIVD 250 260 270 280 290 300 TGYGLERFVW ASKGSPTIYD AVFPDIVREL SDLAGVEHDL HDPEYAEIFA RNARLAGMID 310 320 330 340 350 360 LGEASLRDLR KRIAESINTT PERLERIMAP MERIYAIADH TRCLAYMLGD GIIPSNVKAG 370 380 390 400 410 420 YLARLVIRRT LRMMKDLKLE IPLSEIVEMQ ISKLDYDDWR ERMETISEIL SLEEERYAET 430 440 450 460 470 480 LEKGSRMVSK IASHYSKKGG RIPLTELVSL YDTHGIPPEI ARETAGALGV DVELPDNFYS 490 500 510 520 530 540 IVASTHSRAE QREVETRSPP FEKTERLFYY RPFDQEFDAT VLGIFEGSVV LDRTLFYPEG 550 560 570 580 590 600 GGQPADRGVL VRDGQVFNVN DVQMIDGVVL HRVEQEGLSP GDRVTGRIDM RRRMAHARHH 610 620 630 640 650 660 TATHIVNDSA KRVLGRHVWQ AGAQKSEDRA RLDISHYRRI SDEELKAIEL EANRRVMEMI 670 680 690 700 710 720 PVITEFMPRE EAERLFGFQL YQGGVPPGRE IRVVRVGSDI EACAGTHVTN TGMIGPIKIL 730 740 750 760 770 780 RTERVQDGVE RIEFAAGEAA VQRIQERDDI LAEAASILRV PIEQLPRTVF RFFEEWKDQQ 790 800 810 820 830 840 KVIEHLKEEI AGIRILTLSS EAVDVNGVSI VARDMGESDG ETLLKAATML SERDITAILG 850 860 870 880 890 900 GASGGAAKIV VSVGRSGLER GLNAADIVRA AAKYIGGGGG GKPDLAQGGG PNVGGLRAAI 910 DAGMSAARKA LQV

References

[1]

"Complete sequence of Methanosaeta thermophila PT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Smith K.S., Ingram-Smith C., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6194 / PT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000477 Genomic DNA. Translation: ABK14447.1.
RefSeq	YP_843087.1. NC_008553.1.
3D structure databases
ProteinModelPortal	A0B6X3.
ModBase	Search...
Protein-protein interaction databases
STRING	A0B6X3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4463151.
GenomeReviews	Gene locus Mthe_0657 in contig CP000477_GR.
KEGG	mtp:Mthe_0657.
NMPDR	fig|349307.7.peg.680.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04130.
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	A0B6X3.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	MTHE349307:MTHE_0657-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_METTP

Accession

Primary (citable) accession number: A0B6X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	November 28, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program