A0B5J1 (GUAAA_METTP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	Mthe_0166

Organism

Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]

Taxonomic identifier

349307 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosaetaceae › Methanosaeta

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000292195
Regions
	Domain	2 – 181	180	Glutamine amidotransferase type-1
Sites
	Active site	72	1	Nucleophile By similarity
	Active site	159	1	By similarity
	Active site	161	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0B5J1 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: A522651347F9F035	FASTA	181	20,218
	10 20 30 40 50 60 MKILVVNNYG QFNHLIYRSI KDRVEARMIS NETPVEDIVA DGLILGGGPS MDRSGRCSEY 70 80 90 100 110 120 LRTLDIPVLG ICLGLQIMAV TFGGKVEPGK VGGYASVEIE VLEEDGILRG VPHRFMAWAS 130 140 150 160 170 180 HADQVTICPP EFRVIARSSI CDIEAICHES RPLYGVQWHP EVAHTEYGEE VFDNFLEICR R

References

[1]

"Complete sequence of Methanosaeta thermophila PT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Smith K.S., Ingram-Smith C., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6194 / PT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000477 Genomic DNA. Translation: ABK13965.1.
RefSeq	YP_842605.1. NC_008553.1.
3D structure databases
ProteinModelPortal	A0B5J1.
ModBase	Search...
Protein-protein interaction databases
STRING	A0B5J1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4462148.
GenomeReviews	Gene locus Mthe_0166 in contig CP000477_GR.
KEGG	mtp:Mthe_0166.
NMPDR	fig|349307.7.peg.171.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05587.
HOGENOM	HBG292341.
OMA	GQYVHRI.
ProtClustDB	PRK00758.
Enzyme and pathway databases
BioCyc	MTHE349307:MTHE_0166-MONOMER.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR017926. GATASE_1. IPR004739. GMP_synth_N. IPR023686. GMP_synthase_A. [Graphical view]
KO	K01951.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_METTP

Accession

Primary (citable) accession number: A0B5J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	November 28, 2006
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program