A0B5C7 (PURL_METTP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Mthe_0099

Organism

Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]

Taxonomic identifier

349307 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosaetaceae › Methanosaeta

Protein attributes

Sequence length	768 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 768	768	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050322
Regions
	Nucleotide binding	111 – 122	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A0B5C7 [UniParc]. Last modified November 28, 2006. Version 1. Checksum: 7AEEEEC6A16327D2	FASTA	768	84,038
	10 20 30 40 50 60 MKVIGLADSE LESSLRKAGI GMSASEARSI ASILGRDPTL AELFCYDAMW SEHCSYKSSR 70 80 90 100 110 120 AVLREFLPTE GPNVVLGPVE DAGIVSIDDE WCVVISHESH NHPSQILPNE GAATGIGGIV 130 140 150 160 170 180 RDVNCMGARV VATADPLRFG DPYGRESSRV RWVAEGVVDG IWQYGNALGV PVIAGDVVFS 190 200 210 220 230 240 RCFDYNCLVN VVAIGVVRRD EIIRSRAPPG SGEKGYDVIL IGKPTDSSGL GGVIFASDTL 250 260 270 280 290 300 REEEEEANRG AVQIPDPFLK NVLFKANEDL FRLVREKGVE IGFKDLGGGG LTCASSEMGA 310 320 330 340 350 360 AGGYGMEIDL DRLHVAGEFP PEVLCIAETQ ERFLIIAPRE LRERILKIYN EDWDLPNVYE 370 380 390 400 410 420 GARASVIGRV TTHDRYIVRH RGEEVVNVPI KHLTGGIRYE RVWRPRIRNL VEPDVRMPED 430 440 450 460 470 480 LNRVMLDMLA SPNIASREHI YRYYDTEVQG NTVIRPGEAD AGLLAPIRER DFGIALSVDS 490 500 510 520 530 540 NPFYGRISPY WGGATAVAEA MRNVAAIGAT PSTLTDCLNF GNPEKPEAFW EFRESVRGLA 550 560 570 580 590 600 DAARNLWLKG HPNQPVPIVS GNVSFYNESP EGAVDPSPVI ACVGIMRDIS RAITMSLKEA 610 620 630 640 650 660 GDGLYLLGPR FDELGGSEYY RLIWGVTGAN VPVVRFPLER SMIYTVIDAV DREILRAAHD 670 680 690 700 710 720 ISNGGMAITA AEMCMLSDHG ISIDISDLGD MRSDKLLFSE SSGFVVEVAD GAEQEFVSIA 730 740 750 760 RSYDLNPVRL GSVSTGDIEM ARDGKMLVRL DPEEAREAWS SGLREAMR

References

[1]

"Complete sequence of Methanosaeta thermophila PT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Smith K.S., Ingram-Smith C., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6194 / PT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000477 Genomic DNA. Translation: ABK13901.1.
RefSeq	YP_842541.1. NC_008553.1.
3D structure databases
ProteinModelPortal	A0B5C7.
ModBase	Search...
Protein-protein interaction databases
STRING	A0B5C7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4462491.
GenomeReviews	Gene locus Mthe_0099 in contig CP000477_GR.
KEGG	mtp:Mthe_0099.
NMPDR	fig|349307.7.peg.103.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	CLSK832955.
Enzyme and pathway databases
BioCyc	MTHE349307:MTHE_0099-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_METTP

Accession

Primary (citable) accession number: A0B5C7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 28, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program