A0B532 (RTCA_METTP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	Mthe_0003

Organism

Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]

Taxonomic identifier

349307 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosaetaceae › Methanosaeta

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.
Sequence caution	The sequence ABK13806.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000325191
Regions
	Nucleotide binding	276 – 280	5	ATP By similarity
Sites
	Active site	300	1	Tele-AMP-histidine intermediate By similarity
	Binding site	100	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0B532 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 0A71FBE0A6613404	FASTA	332	35,742
	10 20 30 40 50 60 MIEIDGSYGE GGGQIVRTSV ALSTLTGIPV RIKNIRRNRP RPGLAAQHVR AIEALAQISR 70 80 90 100 110 120 AETRGVHLGS EEIEFIPGRI SAGSYDVDIG TAGSVTLLIQ CLLPALTAAE GPVTVTVRGG 130 140 150 160 170 180 TDVRWSPTVD YLEHVALPAM HLFGVTATFR CERRGYYPRG GGVVVLSTRP SRLRPARLEL 190 200 210 220 230 240 IEEGICGISH CGSLPEHVAR RQADAALELL KEKGYDARID IQTMSSSSPG SGITLWSGFR 250 260 270 280 290 300 GSSALGERGV RAEDVGREAA KALIDELKSK ASVDVHLADQ LIPYIALAGG EYTTREISSH 310 320 330 TRTNIWTAQR ILRCRIDIDE GEVFRIHSTG SG

References

[1]

"Complete sequence of Methanosaeta thermophila PT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Smith K.S., Ingram-Smith C., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6194 / PT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000477 Genomic DNA. Translation: ABK13806.1. Different initiation.
RefSeq	YP_842446.1. NC_008553.1.
3D structure databases
ProteinModelPortal	A0B532.
ModBase	Search...
Protein-protein interaction databases
STRING	A0B532.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4462858.
GenomeReviews	Gene locus Mthe_0003 in contig CP000477_GR.
KEGG	mtp:Mthe_0003.
NMPDR	fig|349307.7.peg.3.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04553.
HOGENOM	HBG683539.
ProtClustDB	PRK04204.
Enzyme and pathway databases
BioCyc	MTHE349307:MTHE_0003-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_METTP

Accession

Primary (citable) accession number: A0B532

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	January 25, 2012
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program