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A0B532 (RTCA_METTP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase

Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4
Gene names
Name:rtcA
Ordered Locus Names:Mthe_0003
OrganismMethanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT)) [Complete proteome] [HAMAP]
Taxonomic identifier349307 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00200.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Sequence caution

The sequence ABK13806.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
PRO_0000325191

Regions

Nucleotide binding276 – 2805ATP By similarity

Sites

Active site3001Tele-AMP-histidine intermediate By similarity
Binding site1001ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0B532 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: 0A71FBE0A6613404

FASTA33235,742
        10         20         30         40         50         60 
MIEIDGSYGE GGGQIVRTSV ALSTLTGIPV RIKNIRRNRP RPGLAAQHVR AIEALAQISR 

        70         80         90        100        110        120 
AETRGVHLGS EEIEFIPGRI SAGSYDVDIG TAGSVTLLIQ CLLPALTAAE GPVTVTVRGG 

       130        140        150        160        170        180 
TDVRWSPTVD YLEHVALPAM HLFGVTATFR CERRGYYPRG GGVVVLSTRP SRLRPARLEL 

       190        200        210        220        230        240 
IEEGICGISH CGSLPEHVAR RQADAALELL KEKGYDARID IQTMSSSSPG SGITLWSGFR 

       250        260        270        280        290        300 
GSSALGERGV RAEDVGREAA KALIDELKSK ASVDVHLADQ LIPYIALAGG EYTTREISSH 

       310        320        330 
TRTNIWTAQR ILRCRIDIDE GEVFRIHSTG SG 

« Hide

References

[1]"Complete sequence of Methanosaeta thermophila PT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Smith K.S., Ingram-Smith C., Richardson P.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6194 / PT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000477 Genomic DNA. Translation: ABK13806.1. Different initiation.
RefSeqYP_842446.1. NC_008553.1.

3D structure databases

ProteinModelPortalA0B532.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349307.Mthe_0003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK13806; ABK13806; Mthe_0003.
GeneID4462858.
KEGGmtp:Mthe_0003.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0430.
HOGENOMHOG000015264.
KOK01974.
ProtClustDBPRK04204.

Enzyme and pathway databases

BioCycMMET349307:GHKI-4-MONOMER.

Family and domain databases

Gene3D3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPMF_00200. RTC.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMSSF55205. SSF55205. 1 hit.
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCA_METTP
AccessionPrimary (citable) accession number: A0B532
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: February 19, 2014
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families