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A0B532

- RTCA_METTP

UniProt

A0B532 - RTCA_METTP

Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Methanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 2 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

    Catalytic activityi

    ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei100 – 1001ATPUniRule annotation
    Active sitei300 – 3001Tele-AMP-histidine intermediateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi276 – 2805ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMMET349307:GHKI-4-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
    Short name:
    RNA cyclaseUniRule annotation
    Short name:
    RNA-3'-phosphate cyclaseUniRule annotation
    Gene namesi
    Name:rtcAUniRule annotation
    Ordered Locus Names:Mthe_0003
    OrganismiMethanosaeta thermophila (strain DSM 6194 / PT) (Methanothrix thermophila (strain DSM 6194 / PT))
    Taxonomic identifieri349307 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta
    ProteomesiUP000000674: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 332332RNA 3'-terminal phosphate cyclasePRO_0000325191Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi349307.Mthe_0003.

    Structurei

    3D structure databases

    ProteinModelPortaliA0B532.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0430.
    HOGENOMiHOG000015264.
    KOiK01974.

    Family and domain databases

    Gene3Di3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPiMF_00200. RTC.
    InterProiIPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view]
    PANTHERiPTHR11096. PTHR11096. 1 hit.
    PfamiPF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view]
    SUPFAMiSSF55205. SSF55205. 1 hit.
    TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEiPS01287. RTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0B532-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIEIDGSYGE GGGQIVRTSV ALSTLTGIPV RIKNIRRNRP RPGLAAQHVR    50
    AIEALAQISR AETRGVHLGS EEIEFIPGRI SAGSYDVDIG TAGSVTLLIQ 100
    CLLPALTAAE GPVTVTVRGG TDVRWSPTVD YLEHVALPAM HLFGVTATFR 150
    CERRGYYPRG GGVVVLSTRP SRLRPARLEL IEEGICGISH CGSLPEHVAR 200
    RQADAALELL KEKGYDARID IQTMSSSSPG SGITLWSGFR GSSALGERGV 250
    RAEDVGREAA KALIDELKSK ASVDVHLADQ LIPYIALAGG EYTTREISSH 300
    TRTNIWTAQR ILRCRIDIDE GEVFRIHSTG SG 332
    Length:332
    Mass (Da):35,742
    Last modified:March 18, 2008 - v2
    Checksum:i0A71FBE0A6613404
    GO

    Sequence cautioni

    The sequence ABK13806.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000477 Genomic DNA. Translation: ABK13806.1. Different initiation.
    RefSeqiYP_842446.1. NC_008553.1.

    Genome annotation databases

    EnsemblBacteriaiABK13806; ABK13806; Mthe_0003.
    GeneIDi4462858.
    KEGGimtp:Mthe_0003.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000477 Genomic DNA. Translation: ABK13806.1 . Different initiation.
    RefSeqi YP_842446.1. NC_008553.1.

    3D structure databases

    ProteinModelPortali A0B532.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349307.Mthe_0003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK13806 ; ABK13806 ; Mthe_0003 .
    GeneIDi 4462858.
    KEGGi mtp:Mthe_0003.

    Phylogenomic databases

    eggNOGi COG0430.
    HOGENOMi HOG000015264.
    KOi K01974.

    Enzyme and pathway databases

    BioCyci MMET349307:GHKI-4-MONOMER.

    Family and domain databases

    Gene3Di 3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPi MF_00200. RTC.
    InterProi IPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view ]
    PANTHERi PTHR11096. PTHR11096. 1 hit.
    Pfami PF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55205. SSF55205. 1 hit.
    TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEi PS01287. RTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 6194 / PT.

    Entry informationi

    Entry nameiRTCA_METTP
    AccessioniPrimary (citable) accession number: A0B532
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3